Isolation, genetic characterization, and virulence profiling of different Aeromonas species recovered from moribund hybrid catfish (Clarias spp.)
Background and Aim: The high diversity of Aeromonas spp. results in various pathogenicity levels. This group of bacteria causes a serious disease named motile Aeromonas septicemia (MAS) in catfish (Clarias spp.). This study aimed to characterize the species and virulence gene diversity of Aeromonas...
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| Format: | Article |
| Language: | English |
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Veterinary World
2023-09-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | https://www.veterinaryworld.org/Vol.16/September-2023/24.pdf |
| _version_ | 1827808567563911168 |
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| author | Dini Siswani Mulia Rarastoeti Pratiwi Widya Asmara Mohamad Azzam-Sayuti Ina Salwany Md. Yasin Alim Isnansetyo |
| author_facet | Dini Siswani Mulia Rarastoeti Pratiwi Widya Asmara Mohamad Azzam-Sayuti Ina Salwany Md. Yasin Alim Isnansetyo |
| author_sort | Dini Siswani Mulia |
| collection | DOAJ |
| description | Background and Aim: The high diversity of Aeromonas spp. results in various pathogenicity levels. This group of bacteria causes a serious disease named motile Aeromonas septicemia (MAS) in catfish (Clarias spp.). This study aimed to characterize the species and virulence gene diversity of Aeromonas spp. isolated from diseased catfish.
Materials and Methods: Nine Aeromonas spp. were isolated from infected catfish cultivated in Java, Indonesia, and they were identified at the phenotypic and molecular levels (16S rDNA). The virulence genes assessed included aer/haem, alt, ast, flaA, lafA, and fstA.
Results: Phylogenetic analysis identified nine isolates of Aeromonas spp.: Aeromonas hydrophila (11.11%), Aeromonas caviae (11.11%), Aeromonas veronii bv. veronii (44.44%), and Aeromonas dhakensis (33.33%). Virulence genes, such as aer/haem, alt, ast, flaA, lafA, and fstA, were detected in all isolates at frequencies of approximately 100%, 66.67%, 88.89%, 100%, 55.56%, and 66.67%, respectively. This study is the first report on A. dhakensis recovered from an Indonesian catfish culture. Furthermore, our study revealed the presence of A. veronii bv veronii, a biovar that has not been reported before in Indonesia.
Conclusion: This finding confirms that MAS was caused by multiple species of Aeromonas, notably A. dhakensis and A. veronii bv veronii, within Indonesian fish culture. The presence of these Aeromonas species with multiple virulence genes poses a significant threat to the freshwater aquaculture industry. |
| first_indexed | 2024-03-11T22:28:58Z |
| format | Article |
| id | doaj.art-8a620c7d045c4f16b01c3071fc32bb3e |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-03-11T22:28:58Z |
| publishDate | 2023-09-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-8a620c7d045c4f16b01c3071fc32bb3e2023-09-23T13:40:36ZengVeterinary WorldVeterinary World0972-89882231-09162023-09-011691974198410.14202/vetworld.2023.1974-1984Isolation, genetic characterization, and virulence profiling of different Aeromonas species recovered from moribund hybrid catfish (Clarias spp.)Dini Siswani Mulia0https://orcid.org/0000-0001-6751-0660Rarastoeti Pratiwi1https://orcid.org/0000-0002-8421-2056Widya Asmara2https://orcid.org/0000-0001-6386-8892Mohamad Azzam-Sayuti3https://orcid.org/0000-0002-7294-8170Ina Salwany Md. Yasin4https://orcid.org/0000-0003-2905-2915Alim Isnansetyo5https://orcid.org/0000-0002-3219-1185Department of Biology Education, Faculty of Teacher Training and Education, Universitas Muhammadiyah Purwokerto, Jl. K.H. Ahmad Dahlan, Purwokerto 53182, Indonesia.Department of Biology, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Senolowo, Yogyakarta 55281, Indonesia.Department of Biology, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Senolowo, Yogyakarta 55281, Indonesia.Department of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna, Caturtunggal, Yogyakarta 55281, Indonesia.Laboratory of Aquatic Animal Health and Therapeutics, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.Laboratory of Aquatic Animal Health and Therapeutics, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Jl. Flora, Bulaksumur, Yogyakarta 55281, Indonesia.Background and Aim: The high diversity of Aeromonas spp. results in various pathogenicity levels. This group of bacteria causes a serious disease named motile Aeromonas septicemia (MAS) in catfish (Clarias spp.). This study aimed to characterize the species and virulence gene diversity of Aeromonas spp. isolated from diseased catfish. Materials and Methods: Nine Aeromonas spp. were isolated from infected catfish cultivated in Java, Indonesia, and they were identified at the phenotypic and molecular levels (16S rDNA). The virulence genes assessed included aer/haem, alt, ast, flaA, lafA, and fstA. Results: Phylogenetic analysis identified nine isolates of Aeromonas spp.: Aeromonas hydrophila (11.11%), Aeromonas caviae (11.11%), Aeromonas veronii bv. veronii (44.44%), and Aeromonas dhakensis (33.33%). Virulence genes, such as aer/haem, alt, ast, flaA, lafA, and fstA, were detected in all isolates at frequencies of approximately 100%, 66.67%, 88.89%, 100%, 55.56%, and 66.67%, respectively. This study is the first report on A. dhakensis recovered from an Indonesian catfish culture. Furthermore, our study revealed the presence of A. veronii bv veronii, a biovar that has not been reported before in Indonesia. Conclusion: This finding confirms that MAS was caused by multiple species of Aeromonas, notably A. dhakensis and A. veronii bv veronii, within Indonesian fish culture. The presence of these Aeromonas species with multiple virulence genes poses a significant threat to the freshwater aquaculture industry.https://www.veterinaryworld.org/Vol.16/September-2023/24.pdf16s rdnaaeromoniasismotile aeromonas septicemiapathogenicityphenotypephylogenetic |
| spellingShingle | Dini Siswani Mulia Rarastoeti Pratiwi Widya Asmara Mohamad Azzam-Sayuti Ina Salwany Md. Yasin Alim Isnansetyo Isolation, genetic characterization, and virulence profiling of different Aeromonas species recovered from moribund hybrid catfish (Clarias spp.) Veterinary World 16s rdna aeromoniasis motile aeromonas septicemia pathogenicity phenotype phylogenetic |
| title | Isolation, genetic characterization, and virulence profiling of different Aeromonas species recovered from moribund hybrid catfish (Clarias spp.) |
| title_full | Isolation, genetic characterization, and virulence profiling of different Aeromonas species recovered from moribund hybrid catfish (Clarias spp.) |
| title_fullStr | Isolation, genetic characterization, and virulence profiling of different Aeromonas species recovered from moribund hybrid catfish (Clarias spp.) |
| title_full_unstemmed | Isolation, genetic characterization, and virulence profiling of different Aeromonas species recovered from moribund hybrid catfish (Clarias spp.) |
| title_short | Isolation, genetic characterization, and virulence profiling of different Aeromonas species recovered from moribund hybrid catfish (Clarias spp.) |
| title_sort | isolation genetic characterization and virulence profiling of different aeromonas species recovered from moribund hybrid catfish clarias spp |
| topic | 16s rdna aeromoniasis motile aeromonas septicemia pathogenicity phenotype phylogenetic |
| url | https://www.veterinaryworld.org/Vol.16/September-2023/24.pdf |
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