Transcriptome sequencing of Tessaratoma papillosa antennae to identify and analyze expression patterns of putative olfaction genes
Abstract Studies on insect olfaction have increased our understanding of insect’s chemosensory system and chemical ecology, and have improved pest control strategies based on insect behavior. In this study, we assembled the antennal transcriptomes of the lychee giant stink bug, Tessaratoma papillosa...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-03306-7 |
| _version_ | 1818420155702575104 |
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| author | Zhong-Zhen Wu Meng-Qiu Qu Xin-Hua Pu Yang Cui Wan-Yu Xiao Hong-Xia Zhao Shu-Ying Bin Jin-Tian Lin |
| author_facet | Zhong-Zhen Wu Meng-Qiu Qu Xin-Hua Pu Yang Cui Wan-Yu Xiao Hong-Xia Zhao Shu-Ying Bin Jin-Tian Lin |
| author_sort | Zhong-Zhen Wu |
| collection | DOAJ |
| description | Abstract Studies on insect olfaction have increased our understanding of insect’s chemosensory system and chemical ecology, and have improved pest control strategies based on insect behavior. In this study, we assembled the antennal transcriptomes of the lychee giant stink bug, Tessaratoma papillosa, by using next generation sequencing to identify the major olfaction gene families in this species. In total, 59 odorant receptors, 14 ionotropic receptors (8 antennal IRs), and 33 odorant binding proteins (28 classic OBPs and 5 plus-C OBPs) were identified from the male and female antennal transcriptomes. Analyses of tissue expression profiles revealed that all 59 OR transcripts, 2 of the 8 antennal IRs, and 6 of the 33 OBPs were primarily expressed in the antennae, suggesting their putative role in olfaction. The sex-biased expression patterns of these antenna-predominant genes suggested that they may have important functions in the reproductive behavior of these insects. This is the first report that provides a comprehensive resource to future studies on olfaction in the lychee giant stink bug. |
| first_indexed | 2024-12-14T12:49:58Z |
| format | Article |
| id | doaj.art-8a65c42b990f4c0ead2170d2883ec923 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T12:49:58Z |
| publishDate | 2017-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-8a65c42b990f4c0ead2170d2883ec9232022-12-21T23:00:42ZengNature PortfolioScientific Reports2045-23222017-06-017111110.1038/s41598-017-03306-7Transcriptome sequencing of Tessaratoma papillosa antennae to identify and analyze expression patterns of putative olfaction genesZhong-Zhen Wu0Meng-Qiu Qu1Xin-Hua Pu2Yang Cui3Wan-Yu Xiao4Hong-Xia Zhao5Shu-Ying Bin6Jin-Tian Lin7Institute for Management of Invasive Alien Species, 314 Yingdong teaching building, Zhongkai University of Agriculture and EngineeringInstitute for Management of Invasive Alien Species, 314 Yingdong teaching building, Zhongkai University of Agriculture and EngineeringInstitute for Management of Invasive Alien Species, 314 Yingdong teaching building, Zhongkai University of Agriculture and EngineeringInstitute for Management of Invasive Alien Species, 314 Yingdong teaching building, Zhongkai University of Agriculture and EngineeringInstitute for Management of Invasive Alien Species, 314 Yingdong teaching building, Zhongkai University of Agriculture and EngineeringGuangdong Institute of Applied Biological ResourcesInstitute for Management of Invasive Alien Species, 314 Yingdong teaching building, Zhongkai University of Agriculture and EngineeringInstitute for Management of Invasive Alien Species, 314 Yingdong teaching building, Zhongkai University of Agriculture and EngineeringAbstract Studies on insect olfaction have increased our understanding of insect’s chemosensory system and chemical ecology, and have improved pest control strategies based on insect behavior. In this study, we assembled the antennal transcriptomes of the lychee giant stink bug, Tessaratoma papillosa, by using next generation sequencing to identify the major olfaction gene families in this species. In total, 59 odorant receptors, 14 ionotropic receptors (8 antennal IRs), and 33 odorant binding proteins (28 classic OBPs and 5 plus-C OBPs) were identified from the male and female antennal transcriptomes. Analyses of tissue expression profiles revealed that all 59 OR transcripts, 2 of the 8 antennal IRs, and 6 of the 33 OBPs were primarily expressed in the antennae, suggesting their putative role in olfaction. The sex-biased expression patterns of these antenna-predominant genes suggested that they may have important functions in the reproductive behavior of these insects. This is the first report that provides a comprehensive resource to future studies on olfaction in the lychee giant stink bug.https://doi.org/10.1038/s41598-017-03306-7 |
| spellingShingle | Zhong-Zhen Wu Meng-Qiu Qu Xin-Hua Pu Yang Cui Wan-Yu Xiao Hong-Xia Zhao Shu-Ying Bin Jin-Tian Lin Transcriptome sequencing of Tessaratoma papillosa antennae to identify and analyze expression patterns of putative olfaction genes Scientific Reports |
| title | Transcriptome sequencing of Tessaratoma papillosa antennae to identify and analyze expression patterns of putative olfaction genes |
| title_full | Transcriptome sequencing of Tessaratoma papillosa antennae to identify and analyze expression patterns of putative olfaction genes |
| title_fullStr | Transcriptome sequencing of Tessaratoma papillosa antennae to identify and analyze expression patterns of putative olfaction genes |
| title_full_unstemmed | Transcriptome sequencing of Tessaratoma papillosa antennae to identify and analyze expression patterns of putative olfaction genes |
| title_short | Transcriptome sequencing of Tessaratoma papillosa antennae to identify and analyze expression patterns of putative olfaction genes |
| title_sort | transcriptome sequencing of tessaratoma papillosa antennae to identify and analyze expression patterns of putative olfaction genes |
| url | https://doi.org/10.1038/s41598-017-03306-7 |
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