An interobserver reproducibility analysis of Ki67 visual assessment in breast cancer.
Ki67 labeling index (LI) is used as a predictive marker and is associated with prognosis in breast cancer. However, standardised methodologies for measurement are lacking which has limited its application in clinical practice. In this study, we evaluated the interobserver concordance of visual asses...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4416820?pdf=render |
| _version_ | 1811343639301849088 |
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| author | Ruohong Shui Baohua Yu Rui Bi Fei Yang Wentao Yang |
| author_facet | Ruohong Shui Baohua Yu Rui Bi Fei Yang Wentao Yang |
| author_sort | Ruohong Shui |
| collection | DOAJ |
| description | Ki67 labeling index (LI) is used as a predictive marker and is associated with prognosis in breast cancer. However, standardised methodologies for measurement are lacking which has limited its application in clinical practice. In this study, we evaluated the interobserver concordance of visual assessment of Ki67 LI in breast cancer.Ki67-immunostained slides of 160 cases of primary invasive breast cancer were visual assessed by five breast pathologists with two different methods to choose the scoring fields: (1) hot-spot score, (2) average score. Proportions of positive invasive tumor cells at 10 % intervals were scored. The intra-class correlation coefficient (ICC) was used to assess the interobserver reproducibility.(1) A perfect concordance of Ki67 LI was demonstrated according to both score methods (P<0.0001). Average score method (ICC, 0.904) demonstrated a better correlation than hot-spot score method (ICC, 0.894). (2) By respective means according to two score methods, all cases were classified into three groups (≤10%, 11%-30% and >30% Ki-67 LI). The concordance was relatively low in intermediate Ki67 LI group compared with low and high Ki67 LI groups. (3) All cases were classified into three groups by paired-difference (d) between means of hot-spot score and average score (d<5, 5≤d<10, d≥10). The consistency was observed to decrease with increasing paired-difference according to both methods.Visual assessment of Ki67 LI at 10 % intervals is a candidate for a standard method in breast cancer clinical practice. Average score and hot-spot score of visual assessment both demonstrated a perfect concordance, and an overall average assessment across the whole section including hot spots may be a better method. Interobserver concordance of intermediate Ki67 LI in which most cutoffs are located for making clinical decisions was relatively low. |
| first_indexed | 2024-04-13T19:33:25Z |
| format | Article |
| id | doaj.art-8a68cf09fe274da2b00a05b80f41e260 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T19:33:25Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8a68cf09fe274da2b00a05b80f41e2602022-12-22T02:33:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01105e012513110.1371/journal.pone.0125131An interobserver reproducibility analysis of Ki67 visual assessment in breast cancer.Ruohong ShuiBaohua YuRui BiFei YangWentao YangKi67 labeling index (LI) is used as a predictive marker and is associated with prognosis in breast cancer. However, standardised methodologies for measurement are lacking which has limited its application in clinical practice. In this study, we evaluated the interobserver concordance of visual assessment of Ki67 LI in breast cancer.Ki67-immunostained slides of 160 cases of primary invasive breast cancer were visual assessed by five breast pathologists with two different methods to choose the scoring fields: (1) hot-spot score, (2) average score. Proportions of positive invasive tumor cells at 10 % intervals were scored. The intra-class correlation coefficient (ICC) was used to assess the interobserver reproducibility.(1) A perfect concordance of Ki67 LI was demonstrated according to both score methods (P<0.0001). Average score method (ICC, 0.904) demonstrated a better correlation than hot-spot score method (ICC, 0.894). (2) By respective means according to two score methods, all cases were classified into three groups (≤10%, 11%-30% and >30% Ki-67 LI). The concordance was relatively low in intermediate Ki67 LI group compared with low and high Ki67 LI groups. (3) All cases were classified into three groups by paired-difference (d) between means of hot-spot score and average score (d<5, 5≤d<10, d≥10). The consistency was observed to decrease with increasing paired-difference according to both methods.Visual assessment of Ki67 LI at 10 % intervals is a candidate for a standard method in breast cancer clinical practice. Average score and hot-spot score of visual assessment both demonstrated a perfect concordance, and an overall average assessment across the whole section including hot spots may be a better method. Interobserver concordance of intermediate Ki67 LI in which most cutoffs are located for making clinical decisions was relatively low.http://europepmc.org/articles/PMC4416820?pdf=render |
| spellingShingle | Ruohong Shui Baohua Yu Rui Bi Fei Yang Wentao Yang An interobserver reproducibility analysis of Ki67 visual assessment in breast cancer. PLoS ONE |
| title | An interobserver reproducibility analysis of Ki67 visual assessment in breast cancer. |
| title_full | An interobserver reproducibility analysis of Ki67 visual assessment in breast cancer. |
| title_fullStr | An interobserver reproducibility analysis of Ki67 visual assessment in breast cancer. |
| title_full_unstemmed | An interobserver reproducibility analysis of Ki67 visual assessment in breast cancer. |
| title_short | An interobserver reproducibility analysis of Ki67 visual assessment in breast cancer. |
| title_sort | interobserver reproducibility analysis of ki67 visual assessment in breast cancer |
| url | http://europepmc.org/articles/PMC4416820?pdf=render |
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