Evaluation of the Function of the ASFV <i>KP177R</i> Gene, Encoding for Structural Protein p22, in the Process of Virus Replication and in Swine Virulence
African swine fever virus (ASFV) causes a devastating disease of swine that has caused outbreaks in Central Europe since 2007, spreading into Asia in 2018. ASFV is a large, structurally complex virus with a large dsDNA genome encoding for more than 160 genes, most of them still uncharacterized. p22,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/6/986 |
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| author | Elizabeth A. Vuono Elizabeth Ramirez-Medina Sarah Pruitt Ayushi Rai Nallely Espinoza Lauro Velazquez-Salinas Douglas P. Gladue Manuel V. Borca |
| author_facet | Elizabeth A. Vuono Elizabeth Ramirez-Medina Sarah Pruitt Ayushi Rai Nallely Espinoza Lauro Velazquez-Salinas Douglas P. Gladue Manuel V. Borca |
| author_sort | Elizabeth A. Vuono |
| collection | DOAJ |
| description | African swine fever virus (ASFV) causes a devastating disease of swine that has caused outbreaks in Central Europe since 2007, spreading into Asia in 2018. ASFV is a large, structurally complex virus with a large dsDNA genome encoding for more than 160 genes, most of them still uncharacterized. p22, encoded by the ASFV gene KP177R, is an early transcribed, structural virus protein located in the ASFV particle. Although its exact function is unknown, p22 has recently been identified as an interacting partner of several host proteins. Here, we describe the development of a recombinant ASFV (ASFV-G-∆KP177R) lacking the KP177R gene as a tool to evaluate the role of p22 in virus replication and virulence in swine. The recombinant ASFV-G-∆KP177R demonstrated that the KP177R gene is non-essential for ASFV replication in primary swine macrophages, with virus yields similar to those of the parental, highly virulent field isolate Georgia2010 (ASFV-G). In addition, experimental infection of domestic pigs with ASFV-G-∆KP177R produced a clinical disease similar to that caused by the parental ASFV-G. Therefore, and surprisingly, p22 does not seem to be involved in virus replication or virulence in swine. |
| first_indexed | 2024-03-10T11:02:34Z |
| format | Article |
| id | doaj.art-8a69f7c11ff7414283ff91023a066071 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T11:02:34Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-8a69f7c11ff7414283ff91023a0660712023-11-21T21:24:01ZengMDPI AGViruses1999-49152021-05-0113698610.3390/v13060986Evaluation of the Function of the ASFV <i>KP177R</i> Gene, Encoding for Structural Protein p22, in the Process of Virus Replication and in Swine VirulenceElizabeth A. Vuono0Elizabeth Ramirez-Medina1Sarah Pruitt2Ayushi Rai3Nallely Espinoza4Lauro Velazquez-Salinas5Douglas P. Gladue6Manuel V. Borca7Plum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture Greenport, Greenport, NY 11944, USAPlum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture Greenport, Greenport, NY 11944, USAPlum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture Greenport, Greenport, NY 11944, USAPlum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture Greenport, Greenport, NY 11944, USAPlum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture Greenport, Greenport, NY 11944, USAPlum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture Greenport, Greenport, NY 11944, USAPlum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture Greenport, Greenport, NY 11944, USAPlum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture Greenport, Greenport, NY 11944, USAAfrican swine fever virus (ASFV) causes a devastating disease of swine that has caused outbreaks in Central Europe since 2007, spreading into Asia in 2018. ASFV is a large, structurally complex virus with a large dsDNA genome encoding for more than 160 genes, most of them still uncharacterized. p22, encoded by the ASFV gene KP177R, is an early transcribed, structural virus protein located in the ASFV particle. Although its exact function is unknown, p22 has recently been identified as an interacting partner of several host proteins. Here, we describe the development of a recombinant ASFV (ASFV-G-∆KP177R) lacking the KP177R gene as a tool to evaluate the role of p22 in virus replication and virulence in swine. The recombinant ASFV-G-∆KP177R demonstrated that the KP177R gene is non-essential for ASFV replication in primary swine macrophages, with virus yields similar to those of the parental, highly virulent field isolate Georgia2010 (ASFV-G). In addition, experimental infection of domestic pigs with ASFV-G-∆KP177R produced a clinical disease similar to that caused by the parental ASFV-G. Therefore, and surprisingly, p22 does not seem to be involved in virus replication or virulence in swine.https://www.mdpi.com/1999-4915/13/6/986ASFVASFAfrican swine fever virusKP177Rp22 |
| spellingShingle | Elizabeth A. Vuono Elizabeth Ramirez-Medina Sarah Pruitt Ayushi Rai Nallely Espinoza Lauro Velazquez-Salinas Douglas P. Gladue Manuel V. Borca Evaluation of the Function of the ASFV <i>KP177R</i> Gene, Encoding for Structural Protein p22, in the Process of Virus Replication and in Swine Virulence Viruses ASFV ASF African swine fever virus KP177R p22 |
| title | Evaluation of the Function of the ASFV <i>KP177R</i> Gene, Encoding for Structural Protein p22, in the Process of Virus Replication and in Swine Virulence |
| title_full | Evaluation of the Function of the ASFV <i>KP177R</i> Gene, Encoding for Structural Protein p22, in the Process of Virus Replication and in Swine Virulence |
| title_fullStr | Evaluation of the Function of the ASFV <i>KP177R</i> Gene, Encoding for Structural Protein p22, in the Process of Virus Replication and in Swine Virulence |
| title_full_unstemmed | Evaluation of the Function of the ASFV <i>KP177R</i> Gene, Encoding for Structural Protein p22, in the Process of Virus Replication and in Swine Virulence |
| title_short | Evaluation of the Function of the ASFV <i>KP177R</i> Gene, Encoding for Structural Protein p22, in the Process of Virus Replication and in Swine Virulence |
| title_sort | evaluation of the function of the asfv i kp177r i gene encoding for structural protein p22 in the process of virus replication and in swine virulence |
| topic | ASFV ASF African swine fever virus KP177R p22 |
| url | https://www.mdpi.com/1999-4915/13/6/986 |
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