Evaluating fibre orientation dispersion in white matter: Comparison of diffusion MRI, histology and polarized light imaging
Diffusion MRI is an exquisitely sensitive probe of tissue microstructure, and is currently the only non-invasive measure of the brain's fibre architecture. As this technique becomes more sophisticated and microstructurally informative, there is increasing value in comparing diffusion MRI with m...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-08-01
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| Series: | NeuroImage |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1053811917304706 |
| _version_ | 1819144070745817088 |
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| author | Jeroen Mollink Michiel Kleinnijenhuis Anne-Marie van Cappellen van Walsum Stamatios N. Sotiropoulos Michiel Cottaar Christopher Mirfin Mattias P. Heinrich Mark Jenkinson Menuka Pallebage-Gamarallage Olaf Ansorge Saad Jbabdi Karla L. Miller |
| author_facet | Jeroen Mollink Michiel Kleinnijenhuis Anne-Marie van Cappellen van Walsum Stamatios N. Sotiropoulos Michiel Cottaar Christopher Mirfin Mattias P. Heinrich Mark Jenkinson Menuka Pallebage-Gamarallage Olaf Ansorge Saad Jbabdi Karla L. Miller |
| author_sort | Jeroen Mollink |
| collection | DOAJ |
| description | Diffusion MRI is an exquisitely sensitive probe of tissue microstructure, and is currently the only non-invasive measure of the brain's fibre architecture. As this technique becomes more sophisticated and microstructurally informative, there is increasing value in comparing diffusion MRI with microscopic imaging in the same tissue samples. This study compared estimates of fibre orientation dispersion in white matter derived from diffusion MRI to reference measures of dispersion obtained from polarized light imaging and histology.Three post-mortem brain specimens were scanned with diffusion MRI and analyzed with a two-compartment dispersion model. The specimens were then sectioned for microscopy, including polarized light imaging estimates of fibre orientation and histological quantitative estimates of myelin and astrocytes. Dispersion estimates were correlated on region – and voxel-wise levels in the corpus callosum, the centrum semiovale and the corticospinal tract.The region-wise analysis yielded correlation coefficients of r = 0.79 for the diffusion MRI and histology comparison, while r = 0.60 was reported for the comparison with polarized light imaging. In the corpus callosum, we observed a pattern of higher dispersion at the midline compared to its lateral aspects. This pattern was present in all modalities and the dispersion profiles from microscopy and diffusion MRI were highly correlated. The astrocytes appeared to have minor contribution to dispersion observed with diffusion MRI.These results demonstrate that fibre orientation dispersion estimates from diffusion MRI represents the tissue architecture well. Dispersion models might be improved by more faithfully incorporating an informed mapping based on microscopy data. |
| first_indexed | 2024-12-22T12:36:18Z |
| format | Article |
| id | doaj.art-8a6ea5607c384484a87127c6d3cfb08d |
| institution | Directory Open Access Journal |
| issn | 1095-9572 |
| language | English |
| last_indexed | 2024-12-22T12:36:18Z |
| publishDate | 2017-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | NeuroImage |
| spelling | doaj.art-8a6ea5607c384484a87127c6d3cfb08d2022-12-21T18:25:33ZengElsevierNeuroImage1095-95722017-08-01157561574Evaluating fibre orientation dispersion in white matter: Comparison of diffusion MRI, histology and polarized light imagingJeroen Mollink0Michiel Kleinnijenhuis1Anne-Marie van Cappellen van Walsum2Stamatios N. Sotiropoulos3Michiel Cottaar4Christopher Mirfin5Mattias P. Heinrich6Mark Jenkinson7Menuka Pallebage-Gamarallage8Olaf Ansorge9Saad Jbabdi10Karla L. Miller11FMRIB Centre, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, United Kingdom; Department of Anatomy, Donders Institute for Brain, Cognition and Behaviour, Radboud University Medical Center, Nijmegen, The Netherlands; Correspondence to: Department of Anatomy, Radboud UMC, Geert Grooteplein Noord 21, 6500 HB, Nijmegen, The Netherlands.FMRIB Centre, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, United KingdomDepartment of Anatomy, Donders Institute for Brain, Cognition and Behaviour, Radboud University Medical Center, Nijmegen, The NetherlandsFMRIB Centre, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, United Kingdom; Sir Peter Mansfield Imaging Centre, School of Medicine, University of Nottingham, Nottingham, United KingdomFMRIB Centre, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, United KingdomFMRIB Centre, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, United Kingdom; Sir Peter Mansfield Imaging Centre, School of Physics and Astronomy, University of Nottingham, Nottingham, United KingdomInstitute of Medical Informatics, Universität zu Lübeck, Lübeck, GermanyFMRIB Centre, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, United KingdomNeuropathology, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, United KingdomNeuropathology, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, United KingdomFMRIB Centre, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, United KingdomFMRIB Centre, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, United Kingdom; Corresponding author.Diffusion MRI is an exquisitely sensitive probe of tissue microstructure, and is currently the only non-invasive measure of the brain's fibre architecture. As this technique becomes more sophisticated and microstructurally informative, there is increasing value in comparing diffusion MRI with microscopic imaging in the same tissue samples. This study compared estimates of fibre orientation dispersion in white matter derived from diffusion MRI to reference measures of dispersion obtained from polarized light imaging and histology.Three post-mortem brain specimens were scanned with diffusion MRI and analyzed with a two-compartment dispersion model. The specimens were then sectioned for microscopy, including polarized light imaging estimates of fibre orientation and histological quantitative estimates of myelin and astrocytes. Dispersion estimates were correlated on region – and voxel-wise levels in the corpus callosum, the centrum semiovale and the corticospinal tract.The region-wise analysis yielded correlation coefficients of r = 0.79 for the diffusion MRI and histology comparison, while r = 0.60 was reported for the comparison with polarized light imaging. In the corpus callosum, we observed a pattern of higher dispersion at the midline compared to its lateral aspects. This pattern was present in all modalities and the dispersion profiles from microscopy and diffusion MRI were highly correlated. The astrocytes appeared to have minor contribution to dispersion observed with diffusion MRI.These results demonstrate that fibre orientation dispersion estimates from diffusion MRI represents the tissue architecture well. Dispersion models might be improved by more faithfully incorporating an informed mapping based on microscopy data.http://www.sciencedirect.com/science/article/pii/S1053811917304706DispersionDiffusion MRIPost-mortemPolarized light imagingMyelinAstrocytes |
| spellingShingle | Jeroen Mollink Michiel Kleinnijenhuis Anne-Marie van Cappellen van Walsum Stamatios N. Sotiropoulos Michiel Cottaar Christopher Mirfin Mattias P. Heinrich Mark Jenkinson Menuka Pallebage-Gamarallage Olaf Ansorge Saad Jbabdi Karla L. Miller Evaluating fibre orientation dispersion in white matter: Comparison of diffusion MRI, histology and polarized light imaging NeuroImage Dispersion Diffusion MRI Post-mortem Polarized light imaging Myelin Astrocytes |
| title | Evaluating fibre orientation dispersion in white matter: Comparison of diffusion MRI, histology and polarized light imaging |
| title_full | Evaluating fibre orientation dispersion in white matter: Comparison of diffusion MRI, histology and polarized light imaging |
| title_fullStr | Evaluating fibre orientation dispersion in white matter: Comparison of diffusion MRI, histology and polarized light imaging |
| title_full_unstemmed | Evaluating fibre orientation dispersion in white matter: Comparison of diffusion MRI, histology and polarized light imaging |
| title_short | Evaluating fibre orientation dispersion in white matter: Comparison of diffusion MRI, histology and polarized light imaging |
| title_sort | evaluating fibre orientation dispersion in white matter comparison of diffusion mri histology and polarized light imaging |
| topic | Dispersion Diffusion MRI Post-mortem Polarized light imaging Myelin Astrocytes |
| url | http://www.sciencedirect.com/science/article/pii/S1053811917304706 |
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