Endosomal signalling via exosome surface TGFβ-1
Extracellular vesicles such as exosomes convey biological messages between cells, either by surface-to-surface interaction or by shuttling of bioactive molecules to a recipient cell’s cytoplasm. Here we show that exosomes released by mast cells harbour both active and latent transforming growth fact...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-12-01
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| Series: | Journal of Extracellular Vesicles |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/20013078.2019.1650458 |
| _version_ | 1819105770501832704 |
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| author | Ganesh Vilas Shelke Yanan Yin Su Chul Jang Cecilia Lässer Stefan Wennmalm Hans Jürgen Hoffmann Li Li Yong Song Gho Jonas Andreas Nilsson Jan Lötvall |
| author_facet | Ganesh Vilas Shelke Yanan Yin Su Chul Jang Cecilia Lässer Stefan Wennmalm Hans Jürgen Hoffmann Li Li Yong Song Gho Jonas Andreas Nilsson Jan Lötvall |
| author_sort | Ganesh Vilas Shelke |
| collection | DOAJ |
| description | Extracellular vesicles such as exosomes convey biological messages between cells, either by surface-to-surface interaction or by shuttling of bioactive molecules to a recipient cell’s cytoplasm. Here we show that exosomes released by mast cells harbour both active and latent transforming growth factor β-1 (TGFβ-1) on their surfaces. The latent form of TGFβ-1 is associated with the exosomes via heparinase-II and pH-sensitive elements. These vesicles traffic to the endocytic compartment of recipient human mesenchymal stem cells (MSCs) within 60 min of exposure. Further, the exosomes-associated TGFβ-1 is retained within the endosomal compartments at the time of signalling, which results in prolonged cellular signalling compared to free-TGFβ-1. These exosomes induce a migratory phenotype in primary MSCs involving SMAD-dependent pathways. Our results show that mast cell-derived exosomes are decorated with latent TGFβ-1 and are retained in recipient MSC endosomes, influencing recipient cell migratory phenotype. We conclude that exosomes can convey signalling within endosomes by delivering bioactive surface ligands to this intracellular compartment. |
| first_indexed | 2024-12-22T02:27:32Z |
| format | Article |
| id | doaj.art-8a704db634814d7081d5caef3ccdb45a |
| institution | Directory Open Access Journal |
| issn | 2001-3078 |
| language | English |
| last_indexed | 2024-12-22T02:27:32Z |
| publishDate | 2019-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Extracellular Vesicles |
| spelling | doaj.art-8a704db634814d7081d5caef3ccdb45a2022-12-21T18:41:58ZengWileyJournal of Extracellular Vesicles2001-30782019-12-018110.1080/20013078.2019.16504581650458Endosomal signalling via exosome surface TGFβ-1Ganesh Vilas Shelke0Yanan Yin1Su Chul Jang2Cecilia Lässer3Stefan Wennmalm4Hans Jürgen Hoffmann5Li Li6Yong Song Gho7Jonas Andreas Nilsson8Jan Lötvall9University of GothenburgUniversity of GothenburgUniversity of GothenburgUniversity of GothenburgExperimental Biomolecular Physics Group, SciLife LaboratoryAarhus UniversityShanghai First People’s Hospital, Shanghai JiaoTong UniversityPohang University of Science and TechnologyInstitute of Clinical Sciences, the Sahlgrenska Academy, University of GothenburgUniversity of GothenburgExtracellular vesicles such as exosomes convey biological messages between cells, either by surface-to-surface interaction or by shuttling of bioactive molecules to a recipient cell’s cytoplasm. Here we show that exosomes released by mast cells harbour both active and latent transforming growth factor β-1 (TGFβ-1) on their surfaces. The latent form of TGFβ-1 is associated with the exosomes via heparinase-II and pH-sensitive elements. These vesicles traffic to the endocytic compartment of recipient human mesenchymal stem cells (MSCs) within 60 min of exposure. Further, the exosomes-associated TGFβ-1 is retained within the endosomal compartments at the time of signalling, which results in prolonged cellular signalling compared to free-TGFβ-1. These exosomes induce a migratory phenotype in primary MSCs involving SMAD-dependent pathways. Our results show that mast cell-derived exosomes are decorated with latent TGFβ-1 and are retained in recipient MSC endosomes, influencing recipient cell migratory phenotype. We conclude that exosomes can convey signalling within endosomes by delivering bioactive surface ligands to this intracellular compartment.http://dx.doi.org/10.1080/20013078.2019.1650458mast cellsextracellular vesiclesexosomesmesenchymal stem cellstumour growth factor beta-1cellular localizationendosomal signallingproteoglycan |
| spellingShingle | Ganesh Vilas Shelke Yanan Yin Su Chul Jang Cecilia Lässer Stefan Wennmalm Hans Jürgen Hoffmann Li Li Yong Song Gho Jonas Andreas Nilsson Jan Lötvall Endosomal signalling via exosome surface TGFβ-1 Journal of Extracellular Vesicles mast cells extracellular vesicles exosomes mesenchymal stem cells tumour growth factor beta-1 cellular localization endosomal signalling proteoglycan |
| title | Endosomal signalling via exosome surface TGFβ-1 |
| title_full | Endosomal signalling via exosome surface TGFβ-1 |
| title_fullStr | Endosomal signalling via exosome surface TGFβ-1 |
| title_full_unstemmed | Endosomal signalling via exosome surface TGFβ-1 |
| title_short | Endosomal signalling via exosome surface TGFβ-1 |
| title_sort | endosomal signalling via exosome surface tgfβ 1 |
| topic | mast cells extracellular vesicles exosomes mesenchymal stem cells tumour growth factor beta-1 cellular localization endosomal signalling proteoglycan |
| url | http://dx.doi.org/10.1080/20013078.2019.1650458 |
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