Characterization of <i>Escherichia coli</i> in Dogs with Pyometra and the Influence of Diet on the Intestinal Colonization of Extraintestinal Pathogenic <i>E. coli</i> (ExPEC)
Despite its high frequency and clinical relevance, the pathogenesis of canine pyometra remains poorly understood. The most accepted hypothesis is that bacteria involved ascend from the intestinal tract, causing the uterine infection. Extraintestinal pathogenic <i>Escherichia coli</i> (Ex...
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MDPI AG
2022-05-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/9/5/245 |
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| author | Rafael Gariglio Clark Xavier Paloma Helena Sanches da Silva Hanna Dornelas Trindade Gabriela Muniz Carvalho Rafael Romero Nicolino Patrícia Maria Coletto Freitas Rodrigo Otávio Silveira Silva |
| author_facet | Rafael Gariglio Clark Xavier Paloma Helena Sanches da Silva Hanna Dornelas Trindade Gabriela Muniz Carvalho Rafael Romero Nicolino Patrícia Maria Coletto Freitas Rodrigo Otávio Silveira Silva |
| author_sort | Rafael Gariglio Clark Xavier |
| collection | DOAJ |
| description | Despite its high frequency and clinical relevance, the pathogenesis of canine pyometra remains poorly understood. The most accepted hypothesis is that bacteria involved ascend from the intestinal tract, causing the uterine infection. Extraintestinal pathogenic <i>Escherichia coli</i> (ExPEC) is the most frequent pathogen in canine pyometra, accounting for 57–100% of cases. The aim of the present study was to determine the frequency of phylogenetic groups and virulence factors in <i>E. coli</i> strains isolated from the uterine and rectal swabs of bitches with pyometra (<i>n</i> = 72) and from rectal swabs from healthy bitches fed commercial dry feed (<i>n</i> = 53) or a raw meat-based diet (RMBD; <i>n</i> = 38). A total of 512 strains of <i>E. coli</i> were isolated and divided into five categories according to the origin of the sample: 120 isolates from the uterine content of dogs with <i>E. coli</i> pyometra, 102 from the feces of bitches with <i>E. coli</i> pyometra, 75 from the feces of bitches without <i>E. coli</i> pyometra, 130 feces samples from healthy dogs fed commercial feed, and 85 feces samples from healthy dogs fed a raw meat-based diet. <i>E. coli</i> strains belonging to the B2 phylogroup and positive for virulence factor genes associated with adhesion (fimbriae type P [<i>papC</i>]) and production of toxins (α-hemolysin [<i>hlyA</i>] and uropathogenic specific protein [<i>usp</i>]) predominated in the uterine content and rectal swabs of bitches with <i>E. coli</i> pyometra. Interestingly, a lower growth rate of <i>E. coli</i> from the B2 phylogroup was observed in dogs fed a RMBD than in those fed commercial dry feed. The present study suggests that intestinal colonization by certain types of <i>E. coli</i> could be a risk factor for the occurrence of <i>E. coli</i> pyometra in bitches and that diet can influence intestinal colonization by such strains. |
| first_indexed | 2024-03-10T01:38:34Z |
| format | Article |
| id | doaj.art-8a72f9e42a7b47e1b7634816da9818b6 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-10T01:38:34Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-8a72f9e42a7b47e1b7634816da9818b62023-11-23T13:28:54ZengMDPI AGVeterinary Sciences2306-73812022-05-019524510.3390/vetsci9050245Characterization of <i>Escherichia coli</i> in Dogs with Pyometra and the Influence of Diet on the Intestinal Colonization of Extraintestinal Pathogenic <i>E. coli</i> (ExPEC)Rafael Gariglio Clark Xavier0Paloma Helena Sanches da Silva1Hanna Dornelas Trindade2Gabriela Muniz Carvalho3Rafael Romero Nicolino4Patrícia Maria Coletto Freitas5Rodrigo Otávio Silveira Silva6Affiliation Veterinary School, Federal University of Minas Gerais, Antônio Carlos Avenue 6627, 31270-090 Belo Horizonte, BrazilAffiliation Veterinary School, Federal University of Minas Gerais, Antônio Carlos Avenue 6627, 31270-090 Belo Horizonte, BrazilAffiliation Veterinary School, Federal University of Minas Gerais, Antônio Carlos Avenue 6627, 31270-090 Belo Horizonte, BrazilAffiliation Veterinary School, Federal University of Minas Gerais, Antônio Carlos Avenue 6627, 31270-090 Belo Horizonte, BrazilAffiliation Veterinary School, Federal University of Minas Gerais, Antônio Carlos Avenue 6627, 31270-090 Belo Horizonte, BrazilAffiliation Veterinary School, Federal University of Minas Gerais, Antônio Carlos Avenue 6627, 31270-090 Belo Horizonte, BrazilAffiliation Veterinary School, Federal University of Minas Gerais, Antônio Carlos Avenue 6627, 31270-090 Belo Horizonte, BrazilDespite its high frequency and clinical relevance, the pathogenesis of canine pyometra remains poorly understood. The most accepted hypothesis is that bacteria involved ascend from the intestinal tract, causing the uterine infection. Extraintestinal pathogenic <i>Escherichia coli</i> (ExPEC) is the most frequent pathogen in canine pyometra, accounting for 57–100% of cases. The aim of the present study was to determine the frequency of phylogenetic groups and virulence factors in <i>E. coli</i> strains isolated from the uterine and rectal swabs of bitches with pyometra (<i>n</i> = 72) and from rectal swabs from healthy bitches fed commercial dry feed (<i>n</i> = 53) or a raw meat-based diet (RMBD; <i>n</i> = 38). A total of 512 strains of <i>E. coli</i> were isolated and divided into five categories according to the origin of the sample: 120 isolates from the uterine content of dogs with <i>E. coli</i> pyometra, 102 from the feces of bitches with <i>E. coli</i> pyometra, 75 from the feces of bitches without <i>E. coli</i> pyometra, 130 feces samples from healthy dogs fed commercial feed, and 85 feces samples from healthy dogs fed a raw meat-based diet. <i>E. coli</i> strains belonging to the B2 phylogroup and positive for virulence factor genes associated with adhesion (fimbriae type P [<i>papC</i>]) and production of toxins (α-hemolysin [<i>hlyA</i>] and uropathogenic specific protein [<i>usp</i>]) predominated in the uterine content and rectal swabs of bitches with <i>E. coli</i> pyometra. Interestingly, a lower growth rate of <i>E. coli</i> from the B2 phylogroup was observed in dogs fed a RMBD than in those fed commercial dry feed. The present study suggests that intestinal colonization by certain types of <i>E. coli</i> could be a risk factor for the occurrence of <i>E. coli</i> pyometra in bitches and that diet can influence intestinal colonization by such strains.https://www.mdpi.com/2306-7381/9/5/245EnPECUPECRMDButerusuterinemicrobiota |
| spellingShingle | Rafael Gariglio Clark Xavier Paloma Helena Sanches da Silva Hanna Dornelas Trindade Gabriela Muniz Carvalho Rafael Romero Nicolino Patrícia Maria Coletto Freitas Rodrigo Otávio Silveira Silva Characterization of <i>Escherichia coli</i> in Dogs with Pyometra and the Influence of Diet on the Intestinal Colonization of Extraintestinal Pathogenic <i>E. coli</i> (ExPEC) Veterinary Sciences EnPEC UPEC RMDB uterus uterine microbiota |
| title | Characterization of <i>Escherichia coli</i> in Dogs with Pyometra and the Influence of Diet on the Intestinal Colonization of Extraintestinal Pathogenic <i>E. coli</i> (ExPEC) |
| title_full | Characterization of <i>Escherichia coli</i> in Dogs with Pyometra and the Influence of Diet on the Intestinal Colonization of Extraintestinal Pathogenic <i>E. coli</i> (ExPEC) |
| title_fullStr | Characterization of <i>Escherichia coli</i> in Dogs with Pyometra and the Influence of Diet on the Intestinal Colonization of Extraintestinal Pathogenic <i>E. coli</i> (ExPEC) |
| title_full_unstemmed | Characterization of <i>Escherichia coli</i> in Dogs with Pyometra and the Influence of Diet on the Intestinal Colonization of Extraintestinal Pathogenic <i>E. coli</i> (ExPEC) |
| title_short | Characterization of <i>Escherichia coli</i> in Dogs with Pyometra and the Influence of Diet on the Intestinal Colonization of Extraintestinal Pathogenic <i>E. coli</i> (ExPEC) |
| title_sort | characterization of i escherichia coli i in dogs with pyometra and the influence of diet on the intestinal colonization of extraintestinal pathogenic i e coli i expec |
| topic | EnPEC UPEC RMDB uterus uterine microbiota |
| url | https://www.mdpi.com/2306-7381/9/5/245 |
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