Serum Amyloid A Concentrations of Healthy and Clinically Diseased Japanese Black Breeding Cattle—Preliminary Measurements for Determining the Cut-Off Concentrations
The present study aimed to compare serum amyloid A (SAA) concentrations of Japanese Black (JB) breeding cows in both clinically normal and diseased cows diagnosed by veterinarians using modified latex agglutination turbidimetric immunoassay (LATIA) to determine the cut-off values for healthy and dis...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/9/5/198 |
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| author | Urara Shinya Yuka Iwamura Osamu Yamato Dhidhi Pambudi Oky Setyo Widodo Masayasu Taniguchi Mitsuhiro Takagi |
| author_facet | Urara Shinya Yuka Iwamura Osamu Yamato Dhidhi Pambudi Oky Setyo Widodo Masayasu Taniguchi Mitsuhiro Takagi |
| author_sort | Urara Shinya |
| collection | DOAJ |
| description | The present study aimed to compare serum amyloid A (SAA) concentrations of Japanese Black (JB) breeding cows in both clinically normal and diseased cows diagnosed by veterinarians using modified latex agglutination turbidimetric immunoassay (LATIA) to determine the cut-off values for healthy and diseased JB cows. For the comparison, a total of 289 serum samples of healthy cows without any clinical symptoms intended for the metabolic profile test and 66 serums from diseased cows clinically diagnosed by veterinarians were measured for the SAA concentrations. A significant difference (<i>p</i>-value = 6.68 × 10<sup>−29</sup>) was observed in the mean SAA concentrations between the healthy (2.8 ± 3.2 mg/L) and diseased (54.8 ± 76.8 mg/L) groups, and the median concentrations of the healthy and diseased groups were 1.5 mg/L and 31.2 mg/L, respectively. Finally, the cut-off SAA concentrations at each probability were 2.9 mg/L (<i>p</i> = 0.05), 5.7 mg/L (<i>p</i> = 0.1), 13.7 mg/L (<i>p</i> = 0.5), and 21.8 mg/L (<i>p</i> = 0.9), respectively, and 6.5 mg/L (<i>p</i> = 0.122) based on evaluation performed using the receiver operating characteristic curve. The results indicated that, with the practical application of the obtained cut-off value, the measurement of SAA concentrations for JB breeding cows with LATIA could be potentially beneficial in the early evaluation of inflammatory diseases in JB breeding cows and possibly useful in the prevention of not only metabolic diseases but also non-nutritional diseases during the perinatal period of JB breeding cows. |
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| language | English |
| last_indexed | 2024-03-10T01:38:34Z |
| publishDate | 2022-04-01 |
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| series | Veterinary Sciences |
| spelling | doaj.art-8a73177129f048ca88482019c52286662023-11-23T13:28:02ZengMDPI AGVeterinary Sciences2306-73812022-04-019519810.3390/vetsci9050198Serum Amyloid A Concentrations of Healthy and Clinically Diseased Japanese Black Breeding Cattle—Preliminary Measurements for Determining the Cut-Off ConcentrationsUrara Shinya0Yuka Iwamura1Osamu Yamato2Dhidhi Pambudi3Oky Setyo Widodo4Masayasu Taniguchi5Mitsuhiro Takagi6Kagoshima Agriculture Mutual Aid Association Soo Branch, Soo 899-8212, JapanKagoshima Agriculture Mutual Aid Association Soo Branch, Soo 899-8212, JapanJoint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, JapanDepartment of Mathematics Education, Faculty of Teacher Training and Education, Sebelas Maret University, Surakata 57126, IndonesiaJoint Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, JapanLaboratory of Theriogenology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, JapanDepartment of Animal Husbandry, Faculty of Veterinary Medicine, Airlangga University, Surabaya 60115, IndonesiaThe present study aimed to compare serum amyloid A (SAA) concentrations of Japanese Black (JB) breeding cows in both clinically normal and diseased cows diagnosed by veterinarians using modified latex agglutination turbidimetric immunoassay (LATIA) to determine the cut-off values for healthy and diseased JB cows. For the comparison, a total of 289 serum samples of healthy cows without any clinical symptoms intended for the metabolic profile test and 66 serums from diseased cows clinically diagnosed by veterinarians were measured for the SAA concentrations. A significant difference (<i>p</i>-value = 6.68 × 10<sup>−29</sup>) was observed in the mean SAA concentrations between the healthy (2.8 ± 3.2 mg/L) and diseased (54.8 ± 76.8 mg/L) groups, and the median concentrations of the healthy and diseased groups were 1.5 mg/L and 31.2 mg/L, respectively. Finally, the cut-off SAA concentrations at each probability were 2.9 mg/L (<i>p</i> = 0.05), 5.7 mg/L (<i>p</i> = 0.1), 13.7 mg/L (<i>p</i> = 0.5), and 21.8 mg/L (<i>p</i> = 0.9), respectively, and 6.5 mg/L (<i>p</i> = 0.122) based on evaluation performed using the receiver operating characteristic curve. The results indicated that, with the practical application of the obtained cut-off value, the measurement of SAA concentrations for JB breeding cows with LATIA could be potentially beneficial in the early evaluation of inflammatory diseases in JB breeding cows and possibly useful in the prevention of not only metabolic diseases but also non-nutritional diseases during the perinatal period of JB breeding cows.https://www.mdpi.com/2306-7381/9/5/198cut-off concentrationsJapanese Blackserum amyloid A |
| spellingShingle | Urara Shinya Yuka Iwamura Osamu Yamato Dhidhi Pambudi Oky Setyo Widodo Masayasu Taniguchi Mitsuhiro Takagi Serum Amyloid A Concentrations of Healthy and Clinically Diseased Japanese Black Breeding Cattle—Preliminary Measurements for Determining the Cut-Off Concentrations Veterinary Sciences cut-off concentrations Japanese Black serum amyloid A |
| title | Serum Amyloid A Concentrations of Healthy and Clinically Diseased Japanese Black Breeding Cattle—Preliminary Measurements for Determining the Cut-Off Concentrations |
| title_full | Serum Amyloid A Concentrations of Healthy and Clinically Diseased Japanese Black Breeding Cattle—Preliminary Measurements for Determining the Cut-Off Concentrations |
| title_fullStr | Serum Amyloid A Concentrations of Healthy and Clinically Diseased Japanese Black Breeding Cattle—Preliminary Measurements for Determining the Cut-Off Concentrations |
| title_full_unstemmed | Serum Amyloid A Concentrations of Healthy and Clinically Diseased Japanese Black Breeding Cattle—Preliminary Measurements for Determining the Cut-Off Concentrations |
| title_short | Serum Amyloid A Concentrations of Healthy and Clinically Diseased Japanese Black Breeding Cattle—Preliminary Measurements for Determining the Cut-Off Concentrations |
| title_sort | serum amyloid a concentrations of healthy and clinically diseased japanese black breeding cattle preliminary measurements for determining the cut off concentrations |
| topic | cut-off concentrations Japanese Black serum amyloid A |
| url | https://www.mdpi.com/2306-7381/9/5/198 |
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