In vitro multiplication of pear tree cultivar Cascatense
One main obstacle to the development and expansion of pear tree cultivation in Brazil has been the lack of cultivars adapted to the edaphoclimatic conditions of regions with the potential for pear production. Intending to overcome this hindrance, Embrapa Clima Temperado (Embrapa Temperate Climate) d...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Londrina
2016-04-01
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| Series: | Semina: Ciências Agrárias |
| Subjects: | |
| Online Access: | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/20385 |
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| author | Ilda Mariclei de Castro da Silva Cristina Copstein Cuchiara Mara Cíntia Winhelmann Valmor João Bianchi Eugenia Jacira Bolacel Braga Leonardo Ferreira Dutra José Antonio Peters |
| author_facet | Ilda Mariclei de Castro da Silva Cristina Copstein Cuchiara Mara Cíntia Winhelmann Valmor João Bianchi Eugenia Jacira Bolacel Braga Leonardo Ferreira Dutra José Antonio Peters |
| author_sort | Ilda Mariclei de Castro da Silva |
| collection | DOAJ |
| description | One main obstacle to the development and expansion of pear tree cultivation in Brazil has been the lack of cultivars adapted to the edaphoclimatic conditions of regions with the potential for pear production. Intending to overcome this hindrance, Embrapa Clima Temperado (Embrapa Temperate Climate) developed and released the cultivar Cascatense, with a low cold requirement (~400 h) better suited for Rio Grande do Sul`s and Santa Catarina`s climate. Still, seedlings of high genetic and sanitary quality are necessary for the establishment of a high-yield orchard. Plants with both sanitary quality and uniformity can be obtained through in vitro micropropagation. Thus, the present study aimed to assess the influence of different culture media (MS, MS¾, WPM, QL, and Himédia), concentrations of sucrose (0, 15, 30, 45, and 60 g L-1), and concentrations of benzylaminopurine (BAP) (0, 0.4, 0.8, 1.2, and 1.6 mg L-1) upon the in vitro multiplication of cultivar Cascatense, to establish an efficient protocol to enhance its propagation and reproducibility. The study included three sequential experiments in which the number of shoots per explant, the length of shoots, the number of axillary buds per shoot, fresh mass, and dry mass of shoots were evaluated. Cultivar Cascatense can be best propagated in vitro with the use of Himédia medium, supplemented with 0.8 mg L-1 of BAP and 30 g L-1 of sucrose. |
| first_indexed | 2024-12-13T06:02:19Z |
| format | Article |
| id | doaj.art-8a749ab5671e4c4cbd964c926f09dbf0 |
| institution | Directory Open Access Journal |
| issn | 1676-546X 1679-0359 |
| language | English |
| last_indexed | 2024-12-13T06:02:19Z |
| publishDate | 2016-04-01 |
| publisher | Universidade Estadual de Londrina |
| record_format | Article |
| series | Semina: Ciências Agrárias |
| spelling | doaj.art-8a749ab5671e4c4cbd964c926f09dbf02022-12-21T23:57:18ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592016-04-0137258159410.5433/1679-0359.2016v37n2p58113580In vitro multiplication of pear tree cultivar CascatenseIlda Mariclei de Castro da Silva0Cristina Copstein Cuchiara1Mara Cíntia Winhelmann2Valmor João BianchiEugenia Jacira Bolacel Braga3Leonardo Ferreira Dutra4José Antonio Peters5Universidade Federal de PelotasUniversidade Federal de PelotasUniversidade Federal de PelotasUniversidade Federal de PelotasEmpresa Brasileira de Pesquisa AgropecuáriaUniversidade Federal de PelotasOne main obstacle to the development and expansion of pear tree cultivation in Brazil has been the lack of cultivars adapted to the edaphoclimatic conditions of regions with the potential for pear production. Intending to overcome this hindrance, Embrapa Clima Temperado (Embrapa Temperate Climate) developed and released the cultivar Cascatense, with a low cold requirement (~400 h) better suited for Rio Grande do Sul`s and Santa Catarina`s climate. Still, seedlings of high genetic and sanitary quality are necessary for the establishment of a high-yield orchard. Plants with both sanitary quality and uniformity can be obtained through in vitro micropropagation. Thus, the present study aimed to assess the influence of different culture media (MS, MS¾, WPM, QL, and Himédia), concentrations of sucrose (0, 15, 30, 45, and 60 g L-1), and concentrations of benzylaminopurine (BAP) (0, 0.4, 0.8, 1.2, and 1.6 mg L-1) upon the in vitro multiplication of cultivar Cascatense, to establish an efficient protocol to enhance its propagation and reproducibility. The study included three sequential experiments in which the number of shoots per explant, the length of shoots, the number of axillary buds per shoot, fresh mass, and dry mass of shoots were evaluated. Cultivar Cascatense can be best propagated in vitro with the use of Himédia medium, supplemented with 0.8 mg L-1 of BAP and 30 g L-1 of sucrose.http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/20385Meio HimédiaSulfato ferrosoSulfato férricoSacaroseBenzilaminopurina. |
| spellingShingle | Ilda Mariclei de Castro da Silva Cristina Copstein Cuchiara Mara Cíntia Winhelmann Valmor João Bianchi Eugenia Jacira Bolacel Braga Leonardo Ferreira Dutra José Antonio Peters In vitro multiplication of pear tree cultivar Cascatense Semina: Ciências Agrárias Meio Himédia Sulfato ferroso Sulfato férrico Sacarose Benzilaminopurina. |
| title | In vitro multiplication of pear tree cultivar Cascatense |
| title_full | In vitro multiplication of pear tree cultivar Cascatense |
| title_fullStr | In vitro multiplication of pear tree cultivar Cascatense |
| title_full_unstemmed | In vitro multiplication of pear tree cultivar Cascatense |
| title_short | In vitro multiplication of pear tree cultivar Cascatense |
| title_sort | in vitro multiplication of pear tree cultivar cascatense |
| topic | Meio Himédia Sulfato ferroso Sulfato férrico Sacarose Benzilaminopurina. |
| url | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/20385 |
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