Bifidobacteria BbC50 Fermentation Products Induce Human Cd4 Regulatory T Cells with Antigen-Specific Activation and Bystander Suppression
Probiotic bacteria have been shown to have health benefits in various situations (inflammation, allergy, infection). We previously showed that a bacteria-free fermentation product of Bifidobacterium breve C50 (BbC50sn) induced high IL-10 secretion by human dendritic cells. As IL-10 is a regulatory c...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2014-01-01
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| Series: | European Journal of Inflammation |
| Online Access: | https://doi.org/10.1177/1721727X1401200116 |
| _version_ | 1818284775336574976 |
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| author | L. Martin A. Granier R. Lemoine A. Dauba S. Vermeersch C. Aubert-Jacquin C. Baron Y. Lebranchu C. Hoarau F. Velge-Roussel |
| author_facet | L. Martin A. Granier R. Lemoine A. Dauba S. Vermeersch C. Aubert-Jacquin C. Baron Y. Lebranchu C. Hoarau F. Velge-Roussel |
| author_sort | L. Martin |
| collection | DOAJ |
| description | Probiotic bacteria have been shown to have health benefits in various situations (inflammation, allergy, infection). We previously showed that a bacteria-free fermentation product of Bifidobacterium breve C50 (BbC50sn) induced high IL-10 secretion by human dendritic cells. As IL-10 is a regulatory cytokine, the aim of the present study was to examine whether DCs cultured in the presence of BbC50sn could induce regulatory T cells in an allogeneic context. Purified CD4 + CD25 − human T cells were co-cultured with allogeneic BbC50sn-treated dendritic cells for 4 weeks. The T cell population (BbC50sn-T) was analysed both at phenotypical and functional [ability to inhibit a mixed lymphocyte reaction (MLR)] levels. We showed that T lymphocytes acquired phenotype characteristics of regulatory T cells after 4 weeks of co-culture with BbC50sn-DCs, and inhibited in vitro T lymphocyte proliferation and IFN-γ production in an MLR. Transwell experiments demonstrated that this suppressive activity was not T cell contact-dependent but probably mediated by a soluble factor. Although BbC50sn-T cells secreted significant amounts of IL-10 and TGF-β, their suppressive effect is most likely not mediated through these cytokines. This is, to our knowledge, the first demonstration of in vitro regulatory T cell induction by a bacteria-free fermentation product in an allogeneic context. |
| first_indexed | 2024-12-13T00:58:10Z |
| format | Article |
| id | doaj.art-8a74fe30c7f9441782089cda8ab21373 |
| institution | Directory Open Access Journal |
| issn | 1721-727X |
| language | English |
| last_indexed | 2024-12-13T00:58:10Z |
| publishDate | 2014-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | European Journal of Inflammation |
| spelling | doaj.art-8a74fe30c7f9441782089cda8ab213732022-12-22T00:04:45ZengSAGE PublishingEuropean Journal of Inflammation1721-727X2014-01-011210.1177/1721727X1401200116Bifidobacteria BbC50 Fermentation Products Induce Human Cd4 Regulatory T Cells with Antigen-Specific Activation and Bystander SuppressionL. Martin0A. Granier1R. Lemoine2A. Dauba3S. Vermeersch4C. Aubert-Jacquin5C. Baron6Y. Lebranchu7C. Hoarau8F. Velge-Roussel9 EA 4245 «Cellules Dendritiques, Immunomodulation et Greffes», UFR de Médecine, Université François-Rabelais de Tours, Tours, France EA 4245 «Cellules Dendritiques, Immunomodulation et Greffes», UFR de Médecine, Université François-Rabelais de Tours, Tours, France EA 4245 «Cellules Dendritiques, Immunomodulation et Greffes», UFR de Médecine, Université François-Rabelais de Tours, Tours, France EA 4245 «Cellules Dendritiques, Immunomodulation et Greffes», UFR de Médecine, Université François-Rabelais de Tours, Tours, France EA 4245 «Cellules Dendritiques, Immunomodulation et Greffes», UFR de Médecine, Université François-Rabelais de Tours, Tours, France Danone Research, Blédina, Vollefranche sur Saône, France Service de Néphrologie et d'Immunologie Clinique, CHRU de Tours, Tours, France Service de Néphrologie et d'Immunologie Clinique, CHRU de Tours, Tours, France EA 4245 «Cellules Dendritiques, Immunomodulation et Greffes», UFR de Médecine, Université François-Rabelais de Tours, Tours, France UFR des Sciences Pharmaceutiques, Tours, FranceProbiotic bacteria have been shown to have health benefits in various situations (inflammation, allergy, infection). We previously showed that a bacteria-free fermentation product of Bifidobacterium breve C50 (BbC50sn) induced high IL-10 secretion by human dendritic cells. As IL-10 is a regulatory cytokine, the aim of the present study was to examine whether DCs cultured in the presence of BbC50sn could induce regulatory T cells in an allogeneic context. Purified CD4 + CD25 − human T cells were co-cultured with allogeneic BbC50sn-treated dendritic cells for 4 weeks. The T cell population (BbC50sn-T) was analysed both at phenotypical and functional [ability to inhibit a mixed lymphocyte reaction (MLR)] levels. We showed that T lymphocytes acquired phenotype characteristics of regulatory T cells after 4 weeks of co-culture with BbC50sn-DCs, and inhibited in vitro T lymphocyte proliferation and IFN-γ production in an MLR. Transwell experiments demonstrated that this suppressive activity was not T cell contact-dependent but probably mediated by a soluble factor. Although BbC50sn-T cells secreted significant amounts of IL-10 and TGF-β, their suppressive effect is most likely not mediated through these cytokines. This is, to our knowledge, the first demonstration of in vitro regulatory T cell induction by a bacteria-free fermentation product in an allogeneic context.https://doi.org/10.1177/1721727X1401200116 |
| spellingShingle | L. Martin A. Granier R. Lemoine A. Dauba S. Vermeersch C. Aubert-Jacquin C. Baron Y. Lebranchu C. Hoarau F. Velge-Roussel Bifidobacteria BbC50 Fermentation Products Induce Human Cd4 Regulatory T Cells with Antigen-Specific Activation and Bystander Suppression European Journal of Inflammation |
| title | Bifidobacteria BbC50 Fermentation Products Induce Human Cd4 Regulatory T Cells with Antigen-Specific Activation and Bystander Suppression |
| title_full | Bifidobacteria BbC50 Fermentation Products Induce Human Cd4 Regulatory T Cells with Antigen-Specific Activation and Bystander Suppression |
| title_fullStr | Bifidobacteria BbC50 Fermentation Products Induce Human Cd4 Regulatory T Cells with Antigen-Specific Activation and Bystander Suppression |
| title_full_unstemmed | Bifidobacteria BbC50 Fermentation Products Induce Human Cd4 Regulatory T Cells with Antigen-Specific Activation and Bystander Suppression |
| title_short | Bifidobacteria BbC50 Fermentation Products Induce Human Cd4 Regulatory T Cells with Antigen-Specific Activation and Bystander Suppression |
| title_sort | bifidobacteria bbc50 fermentation products induce human cd4 regulatory t cells with antigen specific activation and bystander suppression |
| url | https://doi.org/10.1177/1721727X1401200116 |
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