Electroacupuncture Improves Neurobehavioral Function Through Targeting of SOX2-Mediated Axonal Regeneration by MicroRNA-132 After Ischemic Stroke
Our previous studies have shown that electroacupuncture (EA) enhances neurobehavioral functional recovery after ischemic stroke, however, the underlying regulatory mechanisms remain unclear. MicroRNAs (miRNAs) are abundant in the brain and are involved in post-transcriptional gene regulation. During...
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| Format: | Article |
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Frontiers Media S.A.
2018-12-01
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| Series: | Frontiers in Molecular Neuroscience |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fnmol.2018.00471/full |
| _version_ | 1828452356737466368 |
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| author | Xiaoying Zhao Xiaoying Zhao Fuhai Bai Erfei Zhang Erfei Zhang Dandan Zhou Dandan Zhou Tao Jiang Heng Zhou Qiang Wang Qiang Wang |
| author_facet | Xiaoying Zhao Xiaoying Zhao Fuhai Bai Erfei Zhang Erfei Zhang Dandan Zhou Dandan Zhou Tao Jiang Heng Zhou Qiang Wang Qiang Wang |
| author_sort | Xiaoying Zhao |
| collection | DOAJ |
| description | Our previous studies have shown that electroacupuncture (EA) enhances neurobehavioral functional recovery after ischemic stroke, however, the underlying regulatory mechanisms remain unclear. MicroRNAs (miRNAs) are abundant in the brain and are involved in post-transcriptional gene regulation. During cerebral ischemia reperfusion, miRNAs perform numerous biological functions in the central nervous system related to regeneration and repair of damaged nerves. Our previous studies also have shown that the expression of miRNA-132 (miR-132) is obviously down-regulated after stroke by middle cerebral artery occlusion (MCAO), which can be up-regulated by EA. This study aimed to identify whether up-regulation of miR-132 by EA improved the damaged nerves after stroke and to screen the potential target of miR-132. The results showed that EA up-regulated miR-132 thus suppressing SOX2 expression in vivo after MCAO, which obviously ameliorated neurobehavioral functional recovery. Moreover, our results also suggested that up-regulated miR-132 suppressed SOX2 in primary neurons after oxygen-glucose deprivation (OGD), which promoted neurite outgrowth. In conclusion, EA enhances neurobehavioral functional recovery against ischemic stroke through targeting of SOX2-mediated axonal regeneration by miR-132. |
| first_indexed | 2024-12-10T23:52:20Z |
| format | Article |
| id | doaj.art-8a753518cbf04c798657383eac281b66 |
| institution | Directory Open Access Journal |
| issn | 1662-5099 |
| language | English |
| last_indexed | 2024-12-10T23:52:20Z |
| publishDate | 2018-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Neuroscience |
| spelling | doaj.art-8a753518cbf04c798657383eac281b662022-12-22T01:28:43ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992018-12-011110.3389/fnmol.2018.00471412480Electroacupuncture Improves Neurobehavioral Function Through Targeting of SOX2-Mediated Axonal Regeneration by MicroRNA-132 After Ischemic StrokeXiaoying Zhao0Xiaoying Zhao1Fuhai Bai2Erfei Zhang3Erfei Zhang4Dandan Zhou5Dandan Zhou6Tao Jiang7Heng Zhou8Qiang Wang9Qiang Wang10Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, Air Force Medical University, Xi’an, ChinaDepartment of Anesthesiology, Second Hospital of Shanxi Medical University, Taiyuan, ChinaDepartment of Anesthesiology and Perioperative Medicine, Xijing Hospital, Air Force Medical University, Xi’an, ChinaDepartment of Anesthesiology and Perioperative Medicine, Xijing Hospital, Air Force Medical University, Xi’an, ChinaDepartment of Anesthesiology, The Affiliated Hospital of Yan’an University, Yan’an, ChinaDepartment of Anesthesiology and Perioperative Medicine, Xijing Hospital, Air Force Medical University, Xi’an, ChinaDepartment of Anesthesiology, The Northwest Women’s and Children’s Hospital, Xi’an, ChinaDepartment of Anesthesiology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, ChinaDepartment of Anesthesiology and Perioperative Medicine, Xijing Hospital, Air Force Medical University, Xi’an, ChinaDepartment of Anesthesiology and Perioperative Medicine, Xijing Hospital, Air Force Medical University, Xi’an, ChinaDepartment of Anesthesiology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, ChinaOur previous studies have shown that electroacupuncture (EA) enhances neurobehavioral functional recovery after ischemic stroke, however, the underlying regulatory mechanisms remain unclear. MicroRNAs (miRNAs) are abundant in the brain and are involved in post-transcriptional gene regulation. During cerebral ischemia reperfusion, miRNAs perform numerous biological functions in the central nervous system related to regeneration and repair of damaged nerves. Our previous studies also have shown that the expression of miRNA-132 (miR-132) is obviously down-regulated after stroke by middle cerebral artery occlusion (MCAO), which can be up-regulated by EA. This study aimed to identify whether up-regulation of miR-132 by EA improved the damaged nerves after stroke and to screen the potential target of miR-132. The results showed that EA up-regulated miR-132 thus suppressing SOX2 expression in vivo after MCAO, which obviously ameliorated neurobehavioral functional recovery. Moreover, our results also suggested that up-regulated miR-132 suppressed SOX2 in primary neurons after oxygen-glucose deprivation (OGD), which promoted neurite outgrowth. In conclusion, EA enhances neurobehavioral functional recovery against ischemic stroke through targeting of SOX2-mediated axonal regeneration by miR-132.https://www.frontiersin.org/article/10.3389/fnmol.2018.00471/fullstrokeelectroacupuncturemicroRNA-132SOX2neurite outgrowth |
| spellingShingle | Xiaoying Zhao Xiaoying Zhao Fuhai Bai Erfei Zhang Erfei Zhang Dandan Zhou Dandan Zhou Tao Jiang Heng Zhou Qiang Wang Qiang Wang Electroacupuncture Improves Neurobehavioral Function Through Targeting of SOX2-Mediated Axonal Regeneration by MicroRNA-132 After Ischemic Stroke Frontiers in Molecular Neuroscience stroke electroacupuncture microRNA-132 SOX2 neurite outgrowth |
| title | Electroacupuncture Improves Neurobehavioral Function Through Targeting of SOX2-Mediated Axonal Regeneration by MicroRNA-132 After Ischemic Stroke |
| title_full | Electroacupuncture Improves Neurobehavioral Function Through Targeting of SOX2-Mediated Axonal Regeneration by MicroRNA-132 After Ischemic Stroke |
| title_fullStr | Electroacupuncture Improves Neurobehavioral Function Through Targeting of SOX2-Mediated Axonal Regeneration by MicroRNA-132 After Ischemic Stroke |
| title_full_unstemmed | Electroacupuncture Improves Neurobehavioral Function Through Targeting of SOX2-Mediated Axonal Regeneration by MicroRNA-132 After Ischemic Stroke |
| title_short | Electroacupuncture Improves Neurobehavioral Function Through Targeting of SOX2-Mediated Axonal Regeneration by MicroRNA-132 After Ischemic Stroke |
| title_sort | electroacupuncture improves neurobehavioral function through targeting of sox2 mediated axonal regeneration by microrna 132 after ischemic stroke |
| topic | stroke electroacupuncture microRNA-132 SOX2 neurite outgrowth |
| url | https://www.frontiersin.org/article/10.3389/fnmol.2018.00471/full |
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