The ABI3 Transcription Factor Interaction and Antagonism with Ubiquitin E3 Ligase ScPRT1 in <i>Syntrichia caninervis</i>
The ubiquitination pathway has been found to regulate plant responses to environmental stress. However, the role of E3 ubiquitin ligase in desiccation tolerant moss has not yet been elucidated. Previous research has shown that the abscisic acid (ABA) signaling factor ScABI3 can significantly increas...
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MDPI AG
2022-04-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/5/718 |
| _version_ | 1797499646338138112 |
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| author | Yigong Zhang Jiyang Zhou Yi Zhang Daoyuan Zhang |
| author_facet | Yigong Zhang Jiyang Zhou Yi Zhang Daoyuan Zhang |
| author_sort | Yigong Zhang |
| collection | DOAJ |
| description | The ubiquitination pathway has been found to regulate plant responses to environmental stress. However, the role of E3 ubiquitin ligase in desiccation tolerant moss has not yet been elucidated. Previous research has shown that the abscisic acid (ABA) signaling factor ScABI3 can significantly increase desiccation tolerance and reduce ABA sensitivity in the desert moss <i>Syntrichia caninervis</i>. In this study, we identified a RING-type E3 ubiquitin ligase, ScPRT1, and showed that ScABI3 can directly interact with ScPRT1 in vitro and in vivo. Furthermore, we found that the high expression of <i>ScPRT1</i> can interfere with the transcription of <i>ScABI3</i> under ABA treatment. Therefore, we speculate that ScPRT1 may degrade ScABI3 through the ubiquitin-26S proteasome system and participate in ABA-dependent signaling in response to ABA-insensitivity or desiccation tolerance in <i>S. caninervis</i>. The findings from our study may enrich our knowledge of the role of E3 ubiquitin ligase in desiccation tolerance and lay a theoretical foundation for an in-depth study of the relationship between ubiquitination modification and ABA signal transduction under environmental stress. |
| first_indexed | 2024-03-10T03:50:24Z |
| format | Article |
| id | doaj.art-8a763cb5045a453d85cbd1414865d44b |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T03:50:24Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-8a763cb5045a453d85cbd1414865d44b2023-11-23T11:08:22ZengMDPI AGGenes2073-44252022-04-0113571810.3390/genes13050718The ABI3 Transcription Factor Interaction and Antagonism with Ubiquitin E3 Ligase ScPRT1 in <i>Syntrichia caninervis</i>Yigong Zhang0Jiyang Zhou1Yi Zhang2Daoyuan Zhang3Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaXinjiang Key Laboratory of Conservation and Utilization of Plant Gene Resources, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, ChinaThe ubiquitination pathway has been found to regulate plant responses to environmental stress. However, the role of E3 ubiquitin ligase in desiccation tolerant moss has not yet been elucidated. Previous research has shown that the abscisic acid (ABA) signaling factor ScABI3 can significantly increase desiccation tolerance and reduce ABA sensitivity in the desert moss <i>Syntrichia caninervis</i>. In this study, we identified a RING-type E3 ubiquitin ligase, ScPRT1, and showed that ScABI3 can directly interact with ScPRT1 in vitro and in vivo. Furthermore, we found that the high expression of <i>ScPRT1</i> can interfere with the transcription of <i>ScABI3</i> under ABA treatment. Therefore, we speculate that ScPRT1 may degrade ScABI3 through the ubiquitin-26S proteasome system and participate in ABA-dependent signaling in response to ABA-insensitivity or desiccation tolerance in <i>S. caninervis</i>. The findings from our study may enrich our knowledge of the role of E3 ubiquitin ligase in desiccation tolerance and lay a theoretical foundation for an in-depth study of the relationship between ubiquitination modification and ABA signal transduction under environmental stress.https://www.mdpi.com/2073-4425/13/5/718desiccation-tolerant mossE3 ubiquitin ligaseScABI3ScPRT1abscisic acid |
| spellingShingle | Yigong Zhang Jiyang Zhou Yi Zhang Daoyuan Zhang The ABI3 Transcription Factor Interaction and Antagonism with Ubiquitin E3 Ligase ScPRT1 in <i>Syntrichia caninervis</i> Genes desiccation-tolerant moss E3 ubiquitin ligase ScABI3 ScPRT1 abscisic acid |
| title | The ABI3 Transcription Factor Interaction and Antagonism with Ubiquitin E3 Ligase ScPRT1 in <i>Syntrichia caninervis</i> |
| title_full | The ABI3 Transcription Factor Interaction and Antagonism with Ubiquitin E3 Ligase ScPRT1 in <i>Syntrichia caninervis</i> |
| title_fullStr | The ABI3 Transcription Factor Interaction and Antagonism with Ubiquitin E3 Ligase ScPRT1 in <i>Syntrichia caninervis</i> |
| title_full_unstemmed | The ABI3 Transcription Factor Interaction and Antagonism with Ubiquitin E3 Ligase ScPRT1 in <i>Syntrichia caninervis</i> |
| title_short | The ABI3 Transcription Factor Interaction and Antagonism with Ubiquitin E3 Ligase ScPRT1 in <i>Syntrichia caninervis</i> |
| title_sort | abi3 transcription factor interaction and antagonism with ubiquitin e3 ligase scprt1 in i syntrichia caninervis i |
| topic | desiccation-tolerant moss E3 ubiquitin ligase ScABI3 ScPRT1 abscisic acid |
| url | https://www.mdpi.com/2073-4425/13/5/718 |
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