The AP2/ERF GmERF113 Positively Regulates the Drought Response by Activating <i>GmPR10-1</i> in Soybean
Ethylene response factors (ERFs) are involved in biotic and abiotic stress; however, the drought resistance mechanisms of many ERFs in soybeans have not been resolved. Previously, we proved that <i>GmERF113</i> enhances resistance to the pathogen <i>Phytophthora sojae</i> in...
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MDPI AG
2022-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/15/8159 |
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| author | Xin Fang Jia Ma Fengcai Guo Dongyue Qi Ming Zhao Chuanzhong Zhang Le Wang Bo Song Shanshan Liu Shengfu He Yaguang Liu Junjiang Wu Pengfei Xu Shuzhen Zhang |
| author_facet | Xin Fang Jia Ma Fengcai Guo Dongyue Qi Ming Zhao Chuanzhong Zhang Le Wang Bo Song Shanshan Liu Shengfu He Yaguang Liu Junjiang Wu Pengfei Xu Shuzhen Zhang |
| author_sort | Xin Fang |
| collection | DOAJ |
| description | Ethylene response factors (ERFs) are involved in biotic and abiotic stress; however, the drought resistance mechanisms of many ERFs in soybeans have not been resolved. Previously, we proved that <i>GmERF113</i> enhances resistance to the pathogen <i>Phytophthora sojae</i> in soybean. Here, we determined that <i>GmERF113</i> is induced by 20% PEG-6000. Compared to the wild-type plants, soybean plants overexpressing <i>GmERF113</i> (<i>GmERF113</i>-OE) displayed increased drought tolerance which was characterized by milder leaf wilting, less water loss from detached leaves, smaller stomatal aperture, lower Malondialdehyde (MDA) content, increased proline accumulation, and higher Superoxide dismutase (SOD) and Peroxidase (POD) activities under drought stress, whereas plants with <i>GmERF113</i> silenced through RNA interference were the opposite. Chromatin immunoprecipitation and dual effector-reporter assays showed that GmERF113 binds to the GCC-box in the <i>GmPR10-1</i> promoter, activating <i>GmPR10-1</i> expression directly. Overexpressing <i>GmPR10-1</i> improved drought resistance in the composite soybean plants with transgenic hairy roots. RNA-seq analysis revealed that GmERF113 downregulates abscisic acid 8′-hydroxylase 3 (<i>GmABA8</i>’<i>-OH 3</i>) and upregulates various drought-related genes. Overexpressing <i>GmERF113</i> and <i>GmPR10-1</i> increased the abscisic acid (ABA) content and reduced the expression of <i>GmABA8</i>’<i>-OH3</i> in transgenic soybean plants and hairy roots, respectively. These results reveal that the GmERF113-GmPR10-1 pathway improves drought resistance and affects the ABA content in soybean, providing a theoretical basis for the molecular breeding of drought-tolerant soybean. |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T05:23:22Z |
| publishDate | 2022-07-01 |
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| spelling | doaj.art-8a767240689a469391d08f36d5bfc66d2023-12-03T12:38:42ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-07-012315815910.3390/ijms23158159The AP2/ERF GmERF113 Positively Regulates the Drought Response by Activating <i>GmPR10-1</i> in SoybeanXin Fang0Jia Ma1Fengcai Guo2Dongyue Qi3Ming Zhao4Chuanzhong Zhang5Le Wang6Bo Song7Shanshan Liu8Shengfu He9Yaguang Liu10Junjiang Wu11Pengfei Xu12Shuzhen Zhang13Soybean Research Institute of Northeast Agricultural University/Key Laboratory of Soybean Biology of Chinese Education Ministry, Harbin 150030, ChinaSoybean Research Institute of Northeast Agricultural University/Key Laboratory of Soybean Biology of Chinese Education Ministry, Harbin 150030, ChinaSoybean Research Institute of Northeast Agricultural University/Key Laboratory of Soybean Biology of Chinese Education Ministry, Harbin 150030, ChinaSoybean Research Institute of Northeast Agricultural University/Key Laboratory of Soybean Biology of Chinese Education Ministry, Harbin 150030, ChinaSoybean Research Institute of Northeast Agricultural University/Key Laboratory of Soybean Biology of Chinese Education Ministry, Harbin 150030, ChinaSoybean Research Institute of Northeast Agricultural University/Key Laboratory of Soybean Biology of Chinese Education Ministry, Harbin 150030, ChinaSoybean Research Institute of Northeast Agricultural University/Key Laboratory of Soybean Biology of Chinese Education Ministry, Harbin 150030, ChinaSoybean Research Institute of Northeast Agricultural University/Key Laboratory of Soybean Biology of Chinese Education Ministry, Harbin 150030, ChinaSoybean Research Institute of Northeast Agricultural University/Key Laboratory of Soybean Biology of Chinese Education Ministry, Harbin 150030, ChinaSoybean Research Institute of Northeast Agricultural University/Key Laboratory of Soybean Biology of Chinese Education Ministry, Harbin 150030, ChinaSoybean Research Institute of Northeast Agricultural University/Key Laboratory of Soybean Biology of Chinese Education Ministry, Harbin 150030, ChinaSoybean Research Institute of Heilongjiang Academy of Agricultural Sciences/Key Laboratory of Soybean Cultivation of Ministry of Agriculture, Harbin 150030, ChinaSoybean Research Institute of Northeast Agricultural University/Key Laboratory of Soybean Biology of Chinese Education Ministry, Harbin 150030, ChinaSoybean Research Institute of Northeast Agricultural University/Key Laboratory of Soybean Biology of Chinese Education Ministry, Harbin 150030, ChinaEthylene response factors (ERFs) are involved in biotic and abiotic stress; however, the drought resistance mechanisms of many ERFs in soybeans have not been resolved. Previously, we proved that <i>GmERF113</i> enhances resistance to the pathogen <i>Phytophthora sojae</i> in soybean. Here, we determined that <i>GmERF113</i> is induced by 20% PEG-6000. Compared to the wild-type plants, soybean plants overexpressing <i>GmERF113</i> (<i>GmERF113</i>-OE) displayed increased drought tolerance which was characterized by milder leaf wilting, less water loss from detached leaves, smaller stomatal aperture, lower Malondialdehyde (MDA) content, increased proline accumulation, and higher Superoxide dismutase (SOD) and Peroxidase (POD) activities under drought stress, whereas plants with <i>GmERF113</i> silenced through RNA interference were the opposite. Chromatin immunoprecipitation and dual effector-reporter assays showed that GmERF113 binds to the GCC-box in the <i>GmPR10-1</i> promoter, activating <i>GmPR10-1</i> expression directly. Overexpressing <i>GmPR10-1</i> improved drought resistance in the composite soybean plants with transgenic hairy roots. RNA-seq analysis revealed that GmERF113 downregulates abscisic acid 8′-hydroxylase 3 (<i>GmABA8</i>’<i>-OH 3</i>) and upregulates various drought-related genes. Overexpressing <i>GmERF113</i> and <i>GmPR10-1</i> increased the abscisic acid (ABA) content and reduced the expression of <i>GmABA8</i>’<i>-OH3</i> in transgenic soybean plants and hairy roots, respectively. These results reveal that the GmERF113-GmPR10-1 pathway improves drought resistance and affects the ABA content in soybean, providing a theoretical basis for the molecular breeding of drought-tolerant soybean.https://www.mdpi.com/1422-0067/23/15/8159soybeandrought toleranceGmERF113<i>GmPR10-1</i>ABA |
| spellingShingle | Xin Fang Jia Ma Fengcai Guo Dongyue Qi Ming Zhao Chuanzhong Zhang Le Wang Bo Song Shanshan Liu Shengfu He Yaguang Liu Junjiang Wu Pengfei Xu Shuzhen Zhang The AP2/ERF GmERF113 Positively Regulates the Drought Response by Activating <i>GmPR10-1</i> in Soybean International Journal of Molecular Sciences soybean drought tolerance GmERF113 <i>GmPR10-1</i> ABA |
| title | The AP2/ERF GmERF113 Positively Regulates the Drought Response by Activating <i>GmPR10-1</i> in Soybean |
| title_full | The AP2/ERF GmERF113 Positively Regulates the Drought Response by Activating <i>GmPR10-1</i> in Soybean |
| title_fullStr | The AP2/ERF GmERF113 Positively Regulates the Drought Response by Activating <i>GmPR10-1</i> in Soybean |
| title_full_unstemmed | The AP2/ERF GmERF113 Positively Regulates the Drought Response by Activating <i>GmPR10-1</i> in Soybean |
| title_short | The AP2/ERF GmERF113 Positively Regulates the Drought Response by Activating <i>GmPR10-1</i> in Soybean |
| title_sort | ap2 erf gmerf113 positively regulates the drought response by activating i gmpr10 1 i in soybean |
| topic | soybean drought tolerance GmERF113 <i>GmPR10-1</i> ABA |
| url | https://www.mdpi.com/1422-0067/23/15/8159 |
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