Argonaute Family Protein Expression in Normal Tissue and Cancer Entities.
The members of the Argonaute (AGO) protein family are key players in miRNA-guided gene silencing. They enable the interaction between small RNAs and their respective target mRNA(s) and support the catalytic destruction of the gene transcript or recruit additional proteins for downstream gene silenci...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4982624?pdf=render |
| _version_ | 1818014784768966656 |
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| author | Daniel Völler Lisa Linck Astrid Bruckmann Judith Hauptmann Rainer Deutzmann Gunter Meister Anja Katrin Bosserhoff |
| author_facet | Daniel Völler Lisa Linck Astrid Bruckmann Judith Hauptmann Rainer Deutzmann Gunter Meister Anja Katrin Bosserhoff |
| author_sort | Daniel Völler |
| collection | DOAJ |
| description | The members of the Argonaute (AGO) protein family are key players in miRNA-guided gene silencing. They enable the interaction between small RNAs and their respective target mRNA(s) and support the catalytic destruction of the gene transcript or recruit additional proteins for downstream gene silencing. The human AGO family consists of four AGO proteins (AGO1-AGO4), but only AGO2 harbors nuclease activity. In this study, we characterized the expression of the four AGO proteins in cancer cell lines and normal tissues with a new mass spectrometry approach called AGO-APP (AGO Affinity Purification by Peptides). In all analyzed normal tissues, AGO1 and AGO2 were most prominent, but marked tissue-specific differences were identified. Furthermore, considerable changes during development were observed by comparing fetal and adult tissues. We also identified decreased overall AGO expression in melanoma derived cell lines compared to other tumor cell lines and normal tissues, with the largest differences in AGO2 expression. The experiments described in this study suggest that reduced amounts of AGO proteins, as key players in miRNA processing, have impact on several cellular processes. Deregulated miRNA expression has been attributed to chromosomal aberrations, promoter regulation and it is known to have a major impact on tumor development and progression. Our findings will further increase our basic understanding of the molecular basis of miRNA processing and its relevance for disease. |
| first_indexed | 2024-04-14T06:48:37Z |
| format | Article |
| id | doaj.art-8a76b0e0229d4d46899749cb480438c6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-14T06:48:37Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8a76b0e0229d4d46899749cb480438c62022-12-22T02:07:05ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01118e016116510.1371/journal.pone.0161165Argonaute Family Protein Expression in Normal Tissue and Cancer Entities.Daniel VöllerLisa LinckAstrid BruckmannJudith HauptmannRainer DeutzmannGunter MeisterAnja Katrin BosserhoffThe members of the Argonaute (AGO) protein family are key players in miRNA-guided gene silencing. They enable the interaction between small RNAs and their respective target mRNA(s) and support the catalytic destruction of the gene transcript or recruit additional proteins for downstream gene silencing. The human AGO family consists of four AGO proteins (AGO1-AGO4), but only AGO2 harbors nuclease activity. In this study, we characterized the expression of the four AGO proteins in cancer cell lines and normal tissues with a new mass spectrometry approach called AGO-APP (AGO Affinity Purification by Peptides). In all analyzed normal tissues, AGO1 and AGO2 were most prominent, but marked tissue-specific differences were identified. Furthermore, considerable changes during development were observed by comparing fetal and adult tissues. We also identified decreased overall AGO expression in melanoma derived cell lines compared to other tumor cell lines and normal tissues, with the largest differences in AGO2 expression. The experiments described in this study suggest that reduced amounts of AGO proteins, as key players in miRNA processing, have impact on several cellular processes. Deregulated miRNA expression has been attributed to chromosomal aberrations, promoter regulation and it is known to have a major impact on tumor development and progression. Our findings will further increase our basic understanding of the molecular basis of miRNA processing and its relevance for disease.http://europepmc.org/articles/PMC4982624?pdf=render |
| spellingShingle | Daniel Völler Lisa Linck Astrid Bruckmann Judith Hauptmann Rainer Deutzmann Gunter Meister Anja Katrin Bosserhoff Argonaute Family Protein Expression in Normal Tissue and Cancer Entities. PLoS ONE |
| title | Argonaute Family Protein Expression in Normal Tissue and Cancer Entities. |
| title_full | Argonaute Family Protein Expression in Normal Tissue and Cancer Entities. |
| title_fullStr | Argonaute Family Protein Expression in Normal Tissue and Cancer Entities. |
| title_full_unstemmed | Argonaute Family Protein Expression in Normal Tissue and Cancer Entities. |
| title_short | Argonaute Family Protein Expression in Normal Tissue and Cancer Entities. |
| title_sort | argonaute family protein expression in normal tissue and cancer entities |
| url | http://europepmc.org/articles/PMC4982624?pdf=render |
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