The In Vitro Effect of Prostaglandin E<sub>2</sub> and F<sub>2α</sub> on the Chemerin System in the Porcine Endometrium during Gestation
Chemerin belongs to the group of adipocyte-derived hormones known as adipokines, which are responsible mainly for the control of energy homeostasis. Adipokine exerts its influence through three receptors: Chemokine-like receptor 1 (CMKLR1), G protein-coupled receptor 1 (GPR1), and C-C motif chemokin...
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MDPI AG
2020-07-01
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| Series: | International Journal of Molecular Sciences |
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| author | Kamil Dobrzyn Marta Kiezun Ewa Zaobidna Katarzyna Kisielewska Edyta Rytelewska Marlena Gudelska Grzegorz Kopij Kinga Bors Karolina Szymanska Barbara Kaminska Tadeusz Kaminski Nina Smolinska |
| author_facet | Kamil Dobrzyn Marta Kiezun Ewa Zaobidna Katarzyna Kisielewska Edyta Rytelewska Marlena Gudelska Grzegorz Kopij Kinga Bors Karolina Szymanska Barbara Kaminska Tadeusz Kaminski Nina Smolinska |
| author_sort | Kamil Dobrzyn |
| collection | DOAJ |
| description | Chemerin belongs to the group of adipocyte-derived hormones known as adipokines, which are responsible mainly for the control of energy homeostasis. Adipokine exerts its influence through three receptors: Chemokine-like receptor 1 (CMKLR1), G protein-coupled receptor 1 (GPR1), and C-C motif chemokine receptor-like 2 (CCRL2). A growing body of evidence indicates that chemerin participates in the regulation of the female reproductive system. According to the literature, the expression of chemerin and its receptors in reproductive structures depends on the local hormonal milieu. The aim of this study was to investigate the in vitro effect of prostaglandins E<sub>2</sub> (PGE<sub>2</sub>) and F<sub>2α</sub> (PGF<sub>2α</sub>) on chemerin and chemerin receptor (chemerin system) mRNAs (qPCR) and proteins (ELISA, Western blotting) in endometrial tissue explants collected from early-pregnant gilts. Both PGE<sub>2</sub> and PGF<sub>2α</sub> significantly influenced the expression of the chemerin gene, hormone secretion, and the expression of chemerin receptor genes and proteins. The influence of both prostaglandins on the expression of the chemerin system varied between different stages of gestation. This is the first study to describe the modulatory effect of PGE<sub>2</sub> and PGF<sub>2α</sub> on the expression of the chemerin system in the porcine uterus during early gestation. |
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| spelling | doaj.art-8a76e733ad46481f922c1c5ace4cc5b82023-11-20T07:40:37ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-07-012115521310.3390/ijms21155213The In Vitro Effect of Prostaglandin E<sub>2</sub> and F<sub>2α</sub> on the Chemerin System in the Porcine Endometrium during GestationKamil Dobrzyn0Marta Kiezun1Ewa Zaobidna2Katarzyna Kisielewska3Edyta Rytelewska4Marlena Gudelska5Grzegorz Kopij6Kinga Bors7Karolina Szymanska8Barbara Kaminska9Tadeusz Kaminski10Nina Smolinska11Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, PolandDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, PolandDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, PolandDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, PolandDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, PolandDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, PolandDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, PolandDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, PolandDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, PolandDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, PolandDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, PolandDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, PolandChemerin belongs to the group of adipocyte-derived hormones known as adipokines, which are responsible mainly for the control of energy homeostasis. Adipokine exerts its influence through three receptors: Chemokine-like receptor 1 (CMKLR1), G protein-coupled receptor 1 (GPR1), and C-C motif chemokine receptor-like 2 (CCRL2). A growing body of evidence indicates that chemerin participates in the regulation of the female reproductive system. According to the literature, the expression of chemerin and its receptors in reproductive structures depends on the local hormonal milieu. The aim of this study was to investigate the in vitro effect of prostaglandins E<sub>2</sub> (PGE<sub>2</sub>) and F<sub>2α</sub> (PGF<sub>2α</sub>) on chemerin and chemerin receptor (chemerin system) mRNAs (qPCR) and proteins (ELISA, Western blotting) in endometrial tissue explants collected from early-pregnant gilts. Both PGE<sub>2</sub> and PGF<sub>2α</sub> significantly influenced the expression of the chemerin gene, hormone secretion, and the expression of chemerin receptor genes and proteins. The influence of both prostaglandins on the expression of the chemerin system varied between different stages of gestation. This is the first study to describe the modulatory effect of PGE<sub>2</sub> and PGF<sub>2α</sub> on the expression of the chemerin system in the porcine uterus during early gestation.https://www.mdpi.com/1422-0067/21/15/5213early pregnancychemerinchemerin receptorsporcine uterusPGE<sub>2</sub>PGF<sub>2α</sub> |
| spellingShingle | Kamil Dobrzyn Marta Kiezun Ewa Zaobidna Katarzyna Kisielewska Edyta Rytelewska Marlena Gudelska Grzegorz Kopij Kinga Bors Karolina Szymanska Barbara Kaminska Tadeusz Kaminski Nina Smolinska The In Vitro Effect of Prostaglandin E<sub>2</sub> and F<sub>2α</sub> on the Chemerin System in the Porcine Endometrium during Gestation International Journal of Molecular Sciences early pregnancy chemerin chemerin receptors porcine uterus PGE<sub>2</sub> PGF<sub>2α</sub> |
| title | The In Vitro Effect of Prostaglandin E<sub>2</sub> and F<sub>2α</sub> on the Chemerin System in the Porcine Endometrium during Gestation |
| title_full | The In Vitro Effect of Prostaglandin E<sub>2</sub> and F<sub>2α</sub> on the Chemerin System in the Porcine Endometrium during Gestation |
| title_fullStr | The In Vitro Effect of Prostaglandin E<sub>2</sub> and F<sub>2α</sub> on the Chemerin System in the Porcine Endometrium during Gestation |
| title_full_unstemmed | The In Vitro Effect of Prostaglandin E<sub>2</sub> and F<sub>2α</sub> on the Chemerin System in the Porcine Endometrium during Gestation |
| title_short | The In Vitro Effect of Prostaglandin E<sub>2</sub> and F<sub>2α</sub> on the Chemerin System in the Porcine Endometrium during Gestation |
| title_sort | in vitro effect of prostaglandin e sub 2 sub and f sub 2α sub on the chemerin system in the porcine endometrium during gestation |
| topic | early pregnancy chemerin chemerin receptors porcine uterus PGE<sub>2</sub> PGF<sub>2α</sub> |
| url | https://www.mdpi.com/1422-0067/21/15/5213 |
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