Integrated Full-Length Transcriptome and Metabolome Profiling Reveals Flavonoid Regulation in Response to Freezing Stress in Potato
Cold stress impairs plant growth and development, resulting in crop failure. Cultivated potato (<i>Solanum tuberosum</i> L.) is sensitive to freezing, while its wild relative, <i>S. commersonii</i>, has a strong freezing tolerance. To decipher the anti-freezing mechanism of C...
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MDPI AG
2023-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/10/2054 |
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| author | Zhiguo Zhu Lingling Wei Lei Guo Huihui Bao Xuemei Wang Philip Kear Zhen Wang Guangtao Zhu |
| author_facet | Zhiguo Zhu Lingling Wei Lei Guo Huihui Bao Xuemei Wang Philip Kear Zhen Wang Guangtao Zhu |
| author_sort | Zhiguo Zhu |
| collection | DOAJ |
| description | Cold stress impairs plant growth and development, resulting in crop failure. Cultivated potato (<i>Solanum tuberosum</i> L.) is sensitive to freezing, while its wild relative, <i>S. commersonii</i>, has a strong freezing tolerance. To decipher the anti-freezing mechanism of CM, we carried out a transcriptomic and metabolomic analysis of an anti-freezing variety of CM (a type of <i>S. commersonii</i>) and a freeze-sensitive variety of DM (a type of <i>Solanum tuberosum</i> L.). A total of 49,232 high-quality transcripts from 12,811 gene loci, including 46,772 coding sequences and 2018 non-coding RNAs, were identified. KEEG enrichment analysis of differentially expressed genes (DEGs) between the two varieties showed that the flavonoid biosynthesis pathway was strongly induced by freezing stress, which was proven by flavonoid metabolome analysis. Consistent with the accumulation of more flavonoids, nearly all the pathway genes were significantly upregulated in CM than those in DM. The transcript levels of two <i>chalcone synthase</i> (<i>CHS</i>-<i>1</i>) isoforms and four isoforms of <i>flavonoid 3′-hydroxylase</i> (<i>F3′H</i>-<i>1</i>) were confirmed by qRT-PCR. Co-expression analysis identified one <i>Myb-related</i> and three <i>UGT</i>s (<i>UDP</i>-<i>glycosyltransferase</i>) that were significantly upregulated in CM during freezing stress. Our findings support that the flavonoid pathway was significantly enhanced by freezing stress and the greater accumulation ofglycosylatedflavonoids in resistant types than that of sensitive types, maybe accounting for the increased freezing tolerance of freeze-resistant potato varieties. |
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| publishDate | 2023-05-01 |
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| series | Plants |
| spelling | doaj.art-8a76ed19e27a4d88b4cd18e9712156282023-11-18T02:56:58ZengMDPI AGPlants2223-77472023-05-011210205410.3390/plants12102054Integrated Full-Length Transcriptome and Metabolome Profiling Reveals Flavonoid Regulation in Response to Freezing Stress in PotatoZhiguo Zhu0Lingling Wei1Lei Guo2Huihui Bao3Xuemei Wang4Philip Kear5Zhen Wang6Guangtao Zhu7Yunnan Key Laboratory of Potato Biology, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, School of Life Sciences, Yunnan Normal University, Kunming 650500, ChinaYunnan Key Laboratory of Potato Biology, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, School of Life Sciences, Yunnan Normal University, Kunming 650500, ChinaYunnan Key Laboratory of Potato Biology, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, School of Life Sciences, Yunnan Normal University, Kunming 650500, ChinaYunnan Key Laboratory of Potato Biology, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, School of Life Sciences, Yunnan Normal University, Kunming 650500, ChinaYunnan Key Laboratory of Potato Biology, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, School of Life Sciences, Yunnan Normal University, Kunming 650500, ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaInternational Potato Center (CIP), CIP China Center for Asia Pacific, Beijing 100081, ChinaYunnan Key Laboratory of Potato Biology, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, School of Life Sciences, Yunnan Normal University, Kunming 650500, ChinaCold stress impairs plant growth and development, resulting in crop failure. Cultivated potato (<i>Solanum tuberosum</i> L.) is sensitive to freezing, while its wild relative, <i>S. commersonii</i>, has a strong freezing tolerance. To decipher the anti-freezing mechanism of CM, we carried out a transcriptomic and metabolomic analysis of an anti-freezing variety of CM (a type of <i>S. commersonii</i>) and a freeze-sensitive variety of DM (a type of <i>Solanum tuberosum</i> L.). A total of 49,232 high-quality transcripts from 12,811 gene loci, including 46,772 coding sequences and 2018 non-coding RNAs, were identified. KEEG enrichment analysis of differentially expressed genes (DEGs) between the two varieties showed that the flavonoid biosynthesis pathway was strongly induced by freezing stress, which was proven by flavonoid metabolome analysis. Consistent with the accumulation of more flavonoids, nearly all the pathway genes were significantly upregulated in CM than those in DM. The transcript levels of two <i>chalcone synthase</i> (<i>CHS</i>-<i>1</i>) isoforms and four isoforms of <i>flavonoid 3′-hydroxylase</i> (<i>F3′H</i>-<i>1</i>) were confirmed by qRT-PCR. Co-expression analysis identified one <i>Myb-related</i> and three <i>UGT</i>s (<i>UDP</i>-<i>glycosyltransferase</i>) that were significantly upregulated in CM during freezing stress. Our findings support that the flavonoid pathway was significantly enhanced by freezing stress and the greater accumulation ofglycosylatedflavonoids in resistant types than that of sensitive types, maybe accounting for the increased freezing tolerance of freeze-resistant potato varieties.https://www.mdpi.com/2223-7747/12/10/2054<i>S. commersonii</i>freezing stressfull-length transcriptomemetabolomeflavonoid metabolic pathwaytranscription factor |
| spellingShingle | Zhiguo Zhu Lingling Wei Lei Guo Huihui Bao Xuemei Wang Philip Kear Zhen Wang Guangtao Zhu Integrated Full-Length Transcriptome and Metabolome Profiling Reveals Flavonoid Regulation in Response to Freezing Stress in Potato Plants <i>S. commersonii</i> freezing stress full-length transcriptome metabolome flavonoid metabolic pathway transcription factor |
| title | Integrated Full-Length Transcriptome and Metabolome Profiling Reveals Flavonoid Regulation in Response to Freezing Stress in Potato |
| title_full | Integrated Full-Length Transcriptome and Metabolome Profiling Reveals Flavonoid Regulation in Response to Freezing Stress in Potato |
| title_fullStr | Integrated Full-Length Transcriptome and Metabolome Profiling Reveals Flavonoid Regulation in Response to Freezing Stress in Potato |
| title_full_unstemmed | Integrated Full-Length Transcriptome and Metabolome Profiling Reveals Flavonoid Regulation in Response to Freezing Stress in Potato |
| title_short | Integrated Full-Length Transcriptome and Metabolome Profiling Reveals Flavonoid Regulation in Response to Freezing Stress in Potato |
| title_sort | integrated full length transcriptome and metabolome profiling reveals flavonoid regulation in response to freezing stress in potato |
| topic | <i>S. commersonii</i> freezing stress full-length transcriptome metabolome flavonoid metabolic pathway transcription factor |
| url | https://www.mdpi.com/2223-7747/12/10/2054 |
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