Bioplastic (poly-3-hydroxybutyrate)-producing Massilia endophytica sp. nov., isolated from Cannabis sativa L. ‘Cheungsam’
Abstract A rod-shaped, motile, Gram-negative bacterial strain named DM-R-R2A-13T was isolated from the plant Cannabis sativa L. ‘Cheungsam’. The phylogenetic analysis of the 16S rRNA gene sequence revealed that strain DM-R-R2A-13T belongs to the family Oxalobacteraceae and is closely related to memb...
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| Format: | Article |
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Nature Portfolio
2023-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-44976-w |
| _version_ | 1797453286981238784 |
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| author | Doeun Jeon Lingmin Jiang Ki-Hyun Kim Yuxin Peng Donghyun Cho Rae-Dong Jeong Cha Young Kim Jae Cheol Jeong Jiyoung Lee |
| author_facet | Doeun Jeon Lingmin Jiang Ki-Hyun Kim Yuxin Peng Donghyun Cho Rae-Dong Jeong Cha Young Kim Jae Cheol Jeong Jiyoung Lee |
| author_sort | Doeun Jeon |
| collection | DOAJ |
| description | Abstract A rod-shaped, motile, Gram-negative bacterial strain named DM-R-R2A-13T was isolated from the plant Cannabis sativa L. ‘Cheungsam’. The phylogenetic analysis of the 16S rRNA gene sequence revealed that strain DM-R-R2A-13T belongs to the family Oxalobacteraceae and is closely related to members of the genus Massilia, with Massilia flava (97.58% sequence similarity) and Massilia armeniaca (97.37% sequence similarity) being the closest members. The digital DNA-DNA hybridization (dDDH) values between strain DM-R-R2A-13T and Massilia flava CGMCC 1.10685T and Massilia armeniaca ZMN-3Twere 22.2% and 23.3%, while the average nucleotide identity (ANI) values were 78.85% and 79.63%, respectively. The DNA G+C content was measured to be 64.6 mol%. Moreover, the bacterium was found to contain polyhydroxyalkanoate (PHA) granules based on transmission electron microscopy, indicating its potential to produce bioplastic. Genome annotation revealed the presence of PHA synthase genes (phaC, phaR, phaP, and phaZ), and the biopolymer was identified as poly-3-hydroxybutyrate (PHB) based on nuclear magnetic resonance (NMR) and Fourier transform infrared spectroscopy (FTIR) analyses. Using maltose as a carbon source, the strain produced PHB of up to 58.06% of its dry cell weight. Based on the phenotypic, chemotaxonomic, and phylogenetic characteristics, it has been determined that DM-R-R2A-13T represents a novel species belonging to the genus Massilia. As such, the name Massilia endophytica sp. nov. is proposed for this newly identified species. The type strain is DM-R-R2A-13T (= KCTC 92072T = GDMCC 1.2920T). |
| first_indexed | 2024-03-09T15:20:38Z |
| format | Article |
| id | doaj.art-8a775e2358f9466a93ae10379fab7ac6 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:20:38Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-8a775e2358f9466a93ae10379fab7ac62023-11-26T12:49:21ZengNature PortfolioScientific Reports2045-23222023-10-0113111310.1038/s41598-023-44976-wBioplastic (poly-3-hydroxybutyrate)-producing Massilia endophytica sp. nov., isolated from Cannabis sativa L. ‘Cheungsam’Doeun Jeon0Lingmin Jiang1Ki-Hyun Kim2Yuxin Peng3Donghyun Cho4Rae-Dong Jeong5Cha Young Kim6Jae Cheol Jeong7Jiyoung Lee8Korean Collection for Type Cultures (KCTC), Biological Resource Center, Korea Research Institute of Bioscience and BiotechnologyKorean Collection for Type Cultures (KCTC), Biological Resource Center, Korea Research Institute of Bioscience and BiotechnologyKorean Collection for Type Cultures (KCTC), Biological Resource Center, Korea Research Institute of Bioscience and BiotechnologyKorean Collection for Type Cultures (KCTC), Biological Resource Center, Korea Research Institute of Bioscience and BiotechnologyKorean Collection for Type Cultures (KCTC), Biological Resource Center, Korea Research Institute of Bioscience and BiotechnologyDepartment of Applied Biology, Chonnam National UniversityKorean Collection for Type Cultures (KCTC), Biological Resource Center, Korea Research Institute of Bioscience and BiotechnologyKorean Collection for Type Cultures (KCTC), Biological Resource Center, Korea Research Institute of Bioscience and BiotechnologyKorean Collection for Type Cultures (KCTC), Biological Resource Center, Korea Research Institute of Bioscience and BiotechnologyAbstract A rod-shaped, motile, Gram-negative bacterial strain named DM-R-R2A-13T was isolated from the plant Cannabis sativa L. ‘Cheungsam’. The phylogenetic analysis of the 16S rRNA gene sequence revealed that strain DM-R-R2A-13T belongs to the family Oxalobacteraceae and is closely related to members of the genus Massilia, with Massilia flava (97.58% sequence similarity) and Massilia armeniaca (97.37% sequence similarity) being the closest members. The digital DNA-DNA hybridization (dDDH) values between strain DM-R-R2A-13T and Massilia flava CGMCC 1.10685T and Massilia armeniaca ZMN-3Twere 22.2% and 23.3%, while the average nucleotide identity (ANI) values were 78.85% and 79.63%, respectively. The DNA G+C content was measured to be 64.6 mol%. Moreover, the bacterium was found to contain polyhydroxyalkanoate (PHA) granules based on transmission electron microscopy, indicating its potential to produce bioplastic. Genome annotation revealed the presence of PHA synthase genes (phaC, phaR, phaP, and phaZ), and the biopolymer was identified as poly-3-hydroxybutyrate (PHB) based on nuclear magnetic resonance (NMR) and Fourier transform infrared spectroscopy (FTIR) analyses. Using maltose as a carbon source, the strain produced PHB of up to 58.06% of its dry cell weight. Based on the phenotypic, chemotaxonomic, and phylogenetic characteristics, it has been determined that DM-R-R2A-13T represents a novel species belonging to the genus Massilia. As such, the name Massilia endophytica sp. nov. is proposed for this newly identified species. The type strain is DM-R-R2A-13T (= KCTC 92072T = GDMCC 1.2920T).https://doi.org/10.1038/s41598-023-44976-w |
| spellingShingle | Doeun Jeon Lingmin Jiang Ki-Hyun Kim Yuxin Peng Donghyun Cho Rae-Dong Jeong Cha Young Kim Jae Cheol Jeong Jiyoung Lee Bioplastic (poly-3-hydroxybutyrate)-producing Massilia endophytica sp. nov., isolated from Cannabis sativa L. ‘Cheungsam’ Scientific Reports |
| title | Bioplastic (poly-3-hydroxybutyrate)-producing Massilia endophytica sp. nov., isolated from Cannabis sativa L. ‘Cheungsam’ |
| title_full | Bioplastic (poly-3-hydroxybutyrate)-producing Massilia endophytica sp. nov., isolated from Cannabis sativa L. ‘Cheungsam’ |
| title_fullStr | Bioplastic (poly-3-hydroxybutyrate)-producing Massilia endophytica sp. nov., isolated from Cannabis sativa L. ‘Cheungsam’ |
| title_full_unstemmed | Bioplastic (poly-3-hydroxybutyrate)-producing Massilia endophytica sp. nov., isolated from Cannabis sativa L. ‘Cheungsam’ |
| title_short | Bioplastic (poly-3-hydroxybutyrate)-producing Massilia endophytica sp. nov., isolated from Cannabis sativa L. ‘Cheungsam’ |
| title_sort | bioplastic poly 3 hydroxybutyrate producing massilia endophytica sp nov isolated from cannabis sativa l cheungsam |
| url | https://doi.org/10.1038/s41598-023-44976-w |
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