miR-196B-5P and miR-200B-3P Are Differentially Expressed in Medulloblastomas of Adults and Children
Medulloblastoma is a highly aggressive brain tumor that typically affects children, while in adults it represents ~1% of all brain tumors. Little is known about microRNA expression profile of the rare adult medulloblastoma. The main aim of this study was to identify peculiar differences in microRNA...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-04-01
|
| Series: | Diagnostics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4418/10/5/265 |
| _version_ | 1827717807172747264 |
|---|---|
| author | Michela Visani Gianluca Marucci Dario de Biase Felice Giangaspero Francesca Romana Buttarelli Alba Ariela Brandes Enrico Franceschi Giorgia Acquaviva Alessia Ciarrocchi Kerry Jane Rhoden Giovanni Tallini Annalisa Pession |
| author_facet | Michela Visani Gianluca Marucci Dario de Biase Felice Giangaspero Francesca Romana Buttarelli Alba Ariela Brandes Enrico Franceschi Giorgia Acquaviva Alessia Ciarrocchi Kerry Jane Rhoden Giovanni Tallini Annalisa Pession |
| author_sort | Michela Visani |
| collection | DOAJ |
| description | Medulloblastoma is a highly aggressive brain tumor that typically affects children, while in adults it represents ~1% of all brain tumors. Little is known about microRNA expression profile of the rare adult medulloblastoma. The main aim of this study was to identify peculiar differences in microRNA expression between childhood and adult medulloblastoma. Medulloblastomas were profiled for microRNA expression using the Exiqon Human miRNome panel (I + II) analyzing 752 microRNAs in a training set of six adult and six childhood cases. Then, the most differentially expressed microRNAs were validated in a total of 21 adult and 19 childhood cases. Eight microRNAs (miR-196b-5p, miR-183-5p, miR-200b-3p, miR-196a-5p, miR-193a-3p, miR-29c-3p, miR-33b-5p, and miR-200a-3p) were differentially expressed in medulloblastoma of adults and children. Analysis of the validation set confirmed that miR-196b-5p and miR-200b-3p were significantly overexpressed in medulloblastoma of adults as compared with those of children. We followed an in silico approach to investigate direct targets and the pathways involved for the two microRNAs (miR-196b and miR-200b) differently expressed between adult and childhood medulloblastoma. Adult and childhood medulloblastoma have different miRNA expression profiles. In particular, the differential dysregulation of miR-196b-5p and miR-200b-3p characterizes the miRNA profile of adult medulloblastoma and suggests potential targets for novel diagnostic, prognostic, or therapeutic strategies. |
| first_indexed | 2024-03-10T20:09:36Z |
| format | Article |
| id | doaj.art-8a7873e69004475b8488b0008cd65679 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T20:09:36Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-8a7873e69004475b8488b0008cd656792023-11-19T23:01:40ZengMDPI AGDiagnostics2075-44182020-04-0110526510.3390/diagnostics10050265miR-196B-5P and miR-200B-3P Are Differentially Expressed in Medulloblastomas of Adults and ChildrenMichela Visani0Gianluca Marucci1Dario de Biase2Felice Giangaspero3Francesca Romana Buttarelli4Alba Ariela Brandes5Enrico Franceschi6Giorgia Acquaviva7Alessia Ciarrocchi8Kerry Jane Rhoden9Giovanni Tallini10Annalisa Pession11Department of Specialized, Diagnostic and Experimental Medicine, Anatomic Pathology-Molecular Diagnostic Unit AUSL-IRCCS of Bologna, University of Bologna School of Medicine, 40138 Bologna, ItalyAnatomic Pathology Unit, Ospedale Bellaria AUSL-IRCCS of Bologna, 40139 Bologna, ItalyDepartment of Pharmacy and Biotechnology (FaBiT), Molecular Diagnostic Unit AUSL of Bologna, University of Bologna, 40138 Bologna, ItalyDepartment of Radiological, Oncological and Anatomo-Pathological Sciences, Sapienza University School of Medicine, 00161 Rome, ItalyDepartment of Human Neurosciences, Sapienza University School of Medicine, 00161 Rome, ItalyDepartment of Medical Oncology, Bellaria–Maggiore Hospitals AUSL-IRCCS of Bologna, 40139 Bologna, ItalyDepartment of Medical Oncology, Bellaria–Maggiore Hospitals AUSL-IRCCS of Bologna, 40139 Bologna, ItalyDepartment of Specialized, Diagnostic and Experimental Medicine, Anatomic Pathology-Molecular Diagnostic Unit AUSL-IRCCS of Bologna, University of Bologna School of Medicine, 40138 Bologna, ItalyLaboratory of Translational Research, Arcispedale Santa Maria Nuova AUSL-IRCCS of Reggio Emilia, 42122 Reggio Emilia, ItalyDepartment of Medical and Surgical Sciences, Medical Genetics Unit, University of Bologna School of Medicine, 40138 Bologna, ItalyDepartment of Specialized, Diagnostic and Experimental Medicine, Anatomic Pathology-Molecular Diagnostic Unit AUSL-IRCCS of Bologna, University of Bologna School of Medicine, 40138 Bologna, ItalyDepartment of Pharmacy and Biotechnology (FaBiT), Molecular Diagnostic Unit AUSL of Bologna, University of Bologna, 40138 Bologna, ItalyMedulloblastoma is a highly aggressive brain tumor that typically affects children, while in adults it represents ~1% of all brain tumors. Little is known about microRNA expression profile of the rare adult medulloblastoma. The main aim of this study was to identify peculiar differences in microRNA expression between childhood and adult medulloblastoma. Medulloblastomas were profiled for microRNA expression using the Exiqon Human miRNome panel (I + II) analyzing 752 microRNAs in a training set of six adult and six childhood cases. Then, the most differentially expressed microRNAs were validated in a total of 21 adult and 19 childhood cases. Eight microRNAs (miR-196b-5p, miR-183-5p, miR-200b-3p, miR-196a-5p, miR-193a-3p, miR-29c-3p, miR-33b-5p, and miR-200a-3p) were differentially expressed in medulloblastoma of adults and children. Analysis of the validation set confirmed that miR-196b-5p and miR-200b-3p were significantly overexpressed in medulloblastoma of adults as compared with those of children. We followed an in silico approach to investigate direct targets and the pathways involved for the two microRNAs (miR-196b and miR-200b) differently expressed between adult and childhood medulloblastoma. Adult and childhood medulloblastoma have different miRNA expression profiles. In particular, the differential dysregulation of miR-196b-5p and miR-200b-3p characterizes the miRNA profile of adult medulloblastoma and suggests potential targets for novel diagnostic, prognostic, or therapeutic strategies.https://www.mdpi.com/2075-4418/10/5/265microRNAmicroRNA profileadult medulloblastomachildhood medulloblastoma |
| spellingShingle | Michela Visani Gianluca Marucci Dario de Biase Felice Giangaspero Francesca Romana Buttarelli Alba Ariela Brandes Enrico Franceschi Giorgia Acquaviva Alessia Ciarrocchi Kerry Jane Rhoden Giovanni Tallini Annalisa Pession miR-196B-5P and miR-200B-3P Are Differentially Expressed in Medulloblastomas of Adults and Children Diagnostics microRNA microRNA profile adult medulloblastoma childhood medulloblastoma |
| title | miR-196B-5P and miR-200B-3P Are Differentially Expressed in Medulloblastomas of Adults and Children |
| title_full | miR-196B-5P and miR-200B-3P Are Differentially Expressed in Medulloblastomas of Adults and Children |
| title_fullStr | miR-196B-5P and miR-200B-3P Are Differentially Expressed in Medulloblastomas of Adults and Children |
| title_full_unstemmed | miR-196B-5P and miR-200B-3P Are Differentially Expressed in Medulloblastomas of Adults and Children |
| title_short | miR-196B-5P and miR-200B-3P Are Differentially Expressed in Medulloblastomas of Adults and Children |
| title_sort | mir 196b 5p and mir 200b 3p are differentially expressed in medulloblastomas of adults and children |
| topic | microRNA microRNA profile adult medulloblastoma childhood medulloblastoma |
| url | https://www.mdpi.com/2075-4418/10/5/265 |
| work_keys_str_mv | AT michelavisani mir196b5pandmir200b3paredifferentiallyexpressedinmedulloblastomasofadultsandchildren AT gianlucamarucci mir196b5pandmir200b3paredifferentiallyexpressedinmedulloblastomasofadultsandchildren AT dariodebiase mir196b5pandmir200b3paredifferentiallyexpressedinmedulloblastomasofadultsandchildren AT felicegiangaspero mir196b5pandmir200b3paredifferentiallyexpressedinmedulloblastomasofadultsandchildren AT francescaromanabuttarelli mir196b5pandmir200b3paredifferentiallyexpressedinmedulloblastomasofadultsandchildren AT albaarielabrandes mir196b5pandmir200b3paredifferentiallyexpressedinmedulloblastomasofadultsandchildren AT enricofranceschi mir196b5pandmir200b3paredifferentiallyexpressedinmedulloblastomasofadultsandchildren AT giorgiaacquaviva mir196b5pandmir200b3paredifferentiallyexpressedinmedulloblastomasofadultsandchildren AT alessiaciarrocchi mir196b5pandmir200b3paredifferentiallyexpressedinmedulloblastomasofadultsandchildren AT kerryjanerhoden mir196b5pandmir200b3paredifferentiallyexpressedinmedulloblastomasofadultsandchildren AT giovannitallini mir196b5pandmir200b3paredifferentiallyexpressedinmedulloblastomasofadultsandchildren AT annalisapession mir196b5pandmir200b3paredifferentiallyexpressedinmedulloblastomasofadultsandchildren |