B7‐1 and programmed cell death‐ligand 1 in primary and lymph node metastasis lesions of non‐small cell lung carcinoma
Abstract Background Programmed cell death ligand 1 (PD‐L1) status has been reported to be different between metastatic and primary lesions in some cases. Therefore, the interaction between carcinoma and immune cells could influence their expression in the tumor microenvironment. PD‐L1 is known to bi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Cancer Medicine |
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| Online Access: | https://doi.org/10.1002/cam4.4444 |
| _version_ | 1818968334099546112 |
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| author | Takehiro Yamada Yasuhiro Miki Miho Suzuki Osamu Kondoh Ryoko Saito‐Koyama Katsuhiko Ono Yoshinori Okada Hironobu Sasano |
| author_facet | Takehiro Yamada Yasuhiro Miki Miho Suzuki Osamu Kondoh Ryoko Saito‐Koyama Katsuhiko Ono Yoshinori Okada Hironobu Sasano |
| author_sort | Takehiro Yamada |
| collection | DOAJ |
| description | Abstract Background Programmed cell death ligand 1 (PD‐L1) status has been reported to be different between metastatic and primary lesions in some cases. Therefore, the interaction between carcinoma and immune cells could influence their expression in the tumor microenvironment. PD‐L1 is known to bind not only to Programmed cell death 1 (PD‐1) but also to B7‐1 (CD80). In this study, we examined the interaction between lung carcinoma cell lines and peripheral blood mononuclear cells (PBMCs) in vitro. We then examined the significance of B7‐1 expression non‐small cell lung cancer (NSCLC) microenvironment. Methods The interaction of lung carcinoma cell lines and PBMC through the soluble factors was analyzed using a co‐culture system. The changes in expression of immune checkpoint‐related factors in PBMC were examined by PD‐1/PD‐L1 Checkpoint Pathway qPCR Array Kit. B7‐1 expression in NSCLC tissues was examined by immunohistochemistry. Results B7‐1 was upregulated following the co‐culture with the lung carcinoma cell lines. B7‐1 expression in NSCLC tissues was significantly higher in smokers and squamous cell carcinomas and was significantly positively correlated with PD‐L1 status in primary cancer. However, B7‐1 and PD‐1 were not correlated between primary and metastatic diseases in the same patients. Conclusion PD‐1 inhibitors inhibited PD‐1/PD‐L1 binding but not PD‐L1/B7‐1 binding. These results demonstrated that the intratumoral ratio of B7‐1 positive T cells in NSCLC tissue could be involved in the therapeutic efficacy of PD‐L1 inhibitors. This study focused on lymph node metastasis but other sites of distant metastases should be explored by further analysis. |
| first_indexed | 2024-12-20T14:03:02Z |
| format | Article |
| id | doaj.art-8a794d7d86b441d9a01417c384abf3f0 |
| institution | Directory Open Access Journal |
| issn | 2045-7634 |
| language | English |
| last_indexed | 2024-12-20T14:03:02Z |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj.art-8a794d7d86b441d9a01417c384abf3f02022-12-21T19:38:19ZengWileyCancer Medicine2045-76342022-01-0111247949110.1002/cam4.4444B7‐1 and programmed cell death‐ligand 1 in primary and lymph node metastasis lesions of non‐small cell lung carcinomaTakehiro Yamada0Yasuhiro Miki1Miho Suzuki2Osamu Kondoh3Ryoko Saito‐Koyama4Katsuhiko Ono5Yoshinori Okada6Hironobu Sasano7Department of Thoracic Surgery Institute of Development, Aging and Cancer Tohoku University Sendai JapanDepartment of Pathology Tohoku University Graduate School of Medicine Sendai JapanProduct Research Department Chugai Pharmaceutical Co., Ltd Kamakura JapanProduct Research Department Chugai Pharmaceutical Co., Ltd Kamakura JapanDepartment of Pathology Tohoku University Graduate School of Medicine Sendai JapanDepartment of Pathology Tohoku University Graduate School of Medicine Sendai JapanDepartment of Thoracic Surgery Institute of Development, Aging and Cancer Tohoku University Sendai JapanDepartment of Pathology Tohoku University Graduate School of Medicine Sendai JapanAbstract Background Programmed cell death ligand 1 (PD‐L1) status has been reported to be different between metastatic and primary lesions in some cases. Therefore, the interaction between carcinoma and immune cells could influence their expression in the tumor microenvironment. PD‐L1 is known to bind not only to Programmed cell death 1 (PD‐1) but also to B7‐1 (CD80). In this study, we examined the interaction between lung carcinoma cell lines and peripheral blood mononuclear cells (PBMCs) in vitro. We then examined the significance of B7‐1 expression non‐small cell lung cancer (NSCLC) microenvironment. Methods The interaction of lung carcinoma cell lines and PBMC through the soluble factors was analyzed using a co‐culture system. The changes in expression of immune checkpoint‐related factors in PBMC were examined by PD‐1/PD‐L1 Checkpoint Pathway qPCR Array Kit. B7‐1 expression in NSCLC tissues was examined by immunohistochemistry. Results B7‐1 was upregulated following the co‐culture with the lung carcinoma cell lines. B7‐1 expression in NSCLC tissues was significantly higher in smokers and squamous cell carcinomas and was significantly positively correlated with PD‐L1 status in primary cancer. However, B7‐1 and PD‐1 were not correlated between primary and metastatic diseases in the same patients. Conclusion PD‐1 inhibitors inhibited PD‐1/PD‐L1 binding but not PD‐L1/B7‐1 binding. These results demonstrated that the intratumoral ratio of B7‐1 positive T cells in NSCLC tissue could be involved in the therapeutic efficacy of PD‐L1 inhibitors. This study focused on lymph node metastasis but other sites of distant metastases should be explored by further analysis.https://doi.org/10.1002/cam4.4444B7‐1immune check pointlymph node metastasisnon‐small cell lung cancerPD‐L1 |
| spellingShingle | Takehiro Yamada Yasuhiro Miki Miho Suzuki Osamu Kondoh Ryoko Saito‐Koyama Katsuhiko Ono Yoshinori Okada Hironobu Sasano B7‐1 and programmed cell death‐ligand 1 in primary and lymph node metastasis lesions of non‐small cell lung carcinoma Cancer Medicine B7‐1 immune check point lymph node metastasis non‐small cell lung cancer PD‐L1 |
| title | B7‐1 and programmed cell death‐ligand 1 in primary and lymph node metastasis lesions of non‐small cell lung carcinoma |
| title_full | B7‐1 and programmed cell death‐ligand 1 in primary and lymph node metastasis lesions of non‐small cell lung carcinoma |
| title_fullStr | B7‐1 and programmed cell death‐ligand 1 in primary and lymph node metastasis lesions of non‐small cell lung carcinoma |
| title_full_unstemmed | B7‐1 and programmed cell death‐ligand 1 in primary and lymph node metastasis lesions of non‐small cell lung carcinoma |
| title_short | B7‐1 and programmed cell death‐ligand 1 in primary and lymph node metastasis lesions of non‐small cell lung carcinoma |
| title_sort | b7 1 and programmed cell death ligand 1 in primary and lymph node metastasis lesions of non small cell lung carcinoma |
| topic | B7‐1 immune check point lymph node metastasis non‐small cell lung cancer PD‐L1 |
| url | https://doi.org/10.1002/cam4.4444 |
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