Comparison of antibacterial activity and biocompatibility of non-leaching nitrofuran bone cement loaded with vancomycin, gentamicin, and tigecycline
Abstract Background Non-leaching antibacterial bone cement can generate long-term antibacterial activity, it cannot treat serious infections that have occurred like antibiotic-loaded bone cement. Currently, the antibacterial activity and biocompatibility of non-leaching cement when loaded with antib...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-08-01
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| Series: | Journal of Orthopaedic Surgery and Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13018-023-04055-2 |
| _version_ | 1797452045744078848 |
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| author | Zhe Gao Yang Xu Yuchen Kan Hailong Li Rui Guo Luyang Han Wenhan Bu Jianjun Chu |
| author_facet | Zhe Gao Yang Xu Yuchen Kan Hailong Li Rui Guo Luyang Han Wenhan Bu Jianjun Chu |
| author_sort | Zhe Gao |
| collection | DOAJ |
| description | Abstract Background Non-leaching antibacterial bone cement can generate long-term antibacterial activity, it cannot treat serious infections that have occurred like antibiotic-loaded bone cement. Currently, the antibacterial activity and biocompatibility of non-leaching cement when loaded with antibiotics have yet to be determined. Methods Non-leaching antibacterial nitrofuran bone cement (NFBC) specimens were prepared with low-dose and high-dose antibiotics. The antibacterial activity and biocompatibility of NFBC loaded with vancomycin, gentamicin, and tigecycline were compared. The agar diffusion method was employed to observe the inhibition zone of the samples against two bacterial strains from day one to day seven. The CCK-8 assay and acute liver and kidney toxicity test were conducted to assess the effects of the samples on mouse embryo osteoblast precursor cells and C57 mice, respectively. Results Gentamicin-loaded cement exhibited the most potent antibacterial activity, effectively inhibiting both bacterial strains at a low dose. Tigecycline-loaded cement demonstrated superior biocompatibility, showing no acute liver and kidney toxicity in mice and minimal cytotoxicity to osteoblasts. Conclusions NFBC loaded with gentamicin, vancomycin, and tigecycline not only maintains sustained antibacterial activity but also exhibits excellent biocompatibility. |
| first_indexed | 2024-03-09T15:03:05Z |
| format | Article |
| id | doaj.art-8a7b5682d4cf4d31ae115e4c665b40ba |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-03-09T15:03:05Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-8a7b5682d4cf4d31ae115e4c665b40ba2023-11-26T13:48:16ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2023-08-011811810.1186/s13018-023-04055-2Comparison of antibacterial activity and biocompatibility of non-leaching nitrofuran bone cement loaded with vancomycin, gentamicin, and tigecyclineZhe Gao0Yang Xu1Yuchen Kan2Hailong Li3Rui Guo4Luyang Han5Wenhan Bu6Jianjun Chu7Department of Orthopedics, The Second People’s Hospital of Hefei, Hefei Hospital Affiliated to Anhui Medical UniversityDepartment of Orthopedics, The Second People’s Hospital of Hefei, Hefei Hospital Affiliated to Anhui Medical UniversityDepartment of Orthopedics, The Second People’s Hospital of Hefei, Hefei Hospital Affiliated to Anhui Medical UniversityDepartment of Orthopedics, The Second People’s Hospital of FuyangDepartment of Orthopedics, The Second People’s Hospital of Hefei, Hefei Hospital Affiliated to Anhui Medical UniversityDepartment of Orthopedics, The Second People’s Hospital of Hefei, Hefei Hospital Affiliated to Anhui Medical UniversityDepartment of Orthopedics, The Second People’s Hospital of Hefei, Hefei Hospital Affiliated to Anhui Medical UniversityDepartment of Orthopedics, The Second People’s Hospital of Hefei, Hefei Hospital Affiliated to Anhui Medical UniversityAbstract Background Non-leaching antibacterial bone cement can generate long-term antibacterial activity, it cannot treat serious infections that have occurred like antibiotic-loaded bone cement. Currently, the antibacterial activity and biocompatibility of non-leaching cement when loaded with antibiotics have yet to be determined. Methods Non-leaching antibacterial nitrofuran bone cement (NFBC) specimens were prepared with low-dose and high-dose antibiotics. The antibacterial activity and biocompatibility of NFBC loaded with vancomycin, gentamicin, and tigecycline were compared. The agar diffusion method was employed to observe the inhibition zone of the samples against two bacterial strains from day one to day seven. The CCK-8 assay and acute liver and kidney toxicity test were conducted to assess the effects of the samples on mouse embryo osteoblast precursor cells and C57 mice, respectively. Results Gentamicin-loaded cement exhibited the most potent antibacterial activity, effectively inhibiting both bacterial strains at a low dose. Tigecycline-loaded cement demonstrated superior biocompatibility, showing no acute liver and kidney toxicity in mice and minimal cytotoxicity to osteoblasts. Conclusions NFBC loaded with gentamicin, vancomycin, and tigecycline not only maintains sustained antibacterial activity but also exhibits excellent biocompatibility.https://doi.org/10.1186/s13018-023-04055-2Non-leaching antibacterial bone cementAntibiotic-loaded bone cementVancomycinGentamicinTigecycline |
| spellingShingle | Zhe Gao Yang Xu Yuchen Kan Hailong Li Rui Guo Luyang Han Wenhan Bu Jianjun Chu Comparison of antibacterial activity and biocompatibility of non-leaching nitrofuran bone cement loaded with vancomycin, gentamicin, and tigecycline Journal of Orthopaedic Surgery and Research Non-leaching antibacterial bone cement Antibiotic-loaded bone cement Vancomycin Gentamicin Tigecycline |
| title | Comparison of antibacterial activity and biocompatibility of non-leaching nitrofuran bone cement loaded with vancomycin, gentamicin, and tigecycline |
| title_full | Comparison of antibacterial activity and biocompatibility of non-leaching nitrofuran bone cement loaded with vancomycin, gentamicin, and tigecycline |
| title_fullStr | Comparison of antibacterial activity and biocompatibility of non-leaching nitrofuran bone cement loaded with vancomycin, gentamicin, and tigecycline |
| title_full_unstemmed | Comparison of antibacterial activity and biocompatibility of non-leaching nitrofuran bone cement loaded with vancomycin, gentamicin, and tigecycline |
| title_short | Comparison of antibacterial activity and biocompatibility of non-leaching nitrofuran bone cement loaded with vancomycin, gentamicin, and tigecycline |
| title_sort | comparison of antibacterial activity and biocompatibility of non leaching nitrofuran bone cement loaded with vancomycin gentamicin and tigecycline |
| topic | Non-leaching antibacterial bone cement Antibiotic-loaded bone cement Vancomycin Gentamicin Tigecycline |
| url | https://doi.org/10.1186/s13018-023-04055-2 |
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