Characterization of immortalized human dermal microvascular endothelial cells (HMEC-1) for the study of HDL functionality
Abstract Background Primary cultures endothelial cells have been used as models of endothelial related diseases such atherosclerosis. Biological behavior of primary cultures is donor-dependent and data could not be easily reproducible; endothelial cell lines are emerging options, particularly, human...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-03-01
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| Series: | Lipids in Health and Disease |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12944-018-0695-7 |
| _version_ | 1811267995892187136 |
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| author | Mónica Muñoz-Vega Felipe Massó Araceli Páez Elizabeth Carreón-Torres Hector A. Cabrera-Fuentes José Manuel Fragoso Nonanzit Pérez-Hernández Laurent O. Martinez Souad Najib Gilberto Vargas-Alarcón Óscar Pérez-Méndez |
| author_facet | Mónica Muñoz-Vega Felipe Massó Araceli Páez Elizabeth Carreón-Torres Hector A. Cabrera-Fuentes José Manuel Fragoso Nonanzit Pérez-Hernández Laurent O. Martinez Souad Najib Gilberto Vargas-Alarcón Óscar Pérez-Méndez |
| author_sort | Mónica Muñoz-Vega |
| collection | DOAJ |
| description | Abstract Background Primary cultures endothelial cells have been used as models of endothelial related diseases such atherosclerosis. Biological behavior of primary cultures is donor-dependent and data could not be easily reproducible; endothelial cell lines are emerging options, particularly, human dermal microvascular endothelial cells (HMEC-1), that should be validated to substitute primary cultures for the study of HDL functions. Methods Morphology, size and granularity of cells were assessed by phase contrast microscopy and flow cytometry of HMEC-1. The adhesion molecules, ICAM-1and VCAM-1 after TNF-α stimulation, and endothelial markers CD105 endoglin, as well as HDL receptor SR-BI were determined by flow cytometry. Internalization of HDL protein was demonstrated by confocal microscopy using HDL labeled with Alexa Fluor 488. HUVECs were used as reference to compared the characteristics with HMEC-1. Results HMEC-1 and HUVEC had similar morphologies, size and granularity. HMEC-1 expressed endothelial markers as HUVECs, as well as functional SR-B1 receptor since the cell line was able to internalize HDL particles. HMEC-1 effectively increased ICAM-1 and VCAM-1 expression after TNF-α stimulation. HUVECs showed more sensibility to TNF-α stimulus but the range of ICAM-1 and VCAM-1 expression was less homogeneous than in HMEC-1, probably due to biological variation of the former. Finally, the expression of adhesion molecules in HMEC-1 was attenuated by co-incubation with HDL. Conclusion HMEC-1 possess characteristics of endothelial cells, similar to HUVECs, being a cell line suitable to evaluate the functionality of HDL vis-à-vis the endothelium. |
| first_indexed | 2024-04-12T21:13:53Z |
| format | Article |
| id | doaj.art-8a7e309148d540aea49741625208fb7d |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-04-12T21:13:53Z |
| publishDate | 2018-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-8a7e309148d540aea49741625208fb7d2022-12-22T03:16:31ZengBMCLipids in Health and Disease1476-511X2018-03-011711810.1186/s12944-018-0695-7Characterization of immortalized human dermal microvascular endothelial cells (HMEC-1) for the study of HDL functionalityMónica Muñoz-Vega0Felipe Massó1Araceli Páez2Elizabeth Carreón-Torres3Hector A. Cabrera-Fuentes4José Manuel Fragoso5Nonanzit Pérez-Hernández6Laurent O. Martinez7Souad Najib8Gilberto Vargas-Alarcón9Óscar Pérez-Méndez10Molecular Biology Department, Instituto Nacional de Cardiología “Ignacio Chávez”Physiology Departments, Instituto Nacional de Cardiología “Ignacio Chávez”Physiology Departments, Instituto Nacional de Cardiología “Ignacio Chávez”Molecular Biology Department, Instituto Nacional de Cardiología “Ignacio Chávez”Cardiovascular and Metabolic Disorders Program, Duke-NUS Graduate Medical SchoolMolecular Biology Department, Instituto Nacional de Cardiología “Ignacio Chávez”Molecular Biology Department, Instituto Nacional de Cardiología “Ignacio Chávez”Institute of Metabolic and Cardiovascular DiseasesInstitute of Metabolic and Cardiovascular DiseasesMolecular Biology Department, Instituto Nacional de Cardiología “Ignacio Chávez”Molecular Biology Department, Instituto Nacional de Cardiología “Ignacio Chávez”Abstract Background Primary cultures endothelial cells have been used as models of endothelial related diseases such atherosclerosis. Biological behavior of primary cultures is donor-dependent and data could not be easily reproducible; endothelial cell lines are emerging options, particularly, human dermal microvascular endothelial cells (HMEC-1), that should be validated to substitute primary cultures for the study of HDL functions. Methods Morphology, size and granularity of cells were assessed by phase contrast microscopy and flow cytometry of HMEC-1. The adhesion molecules, ICAM-1and VCAM-1 after TNF-α stimulation, and endothelial markers CD105 endoglin, as well as HDL receptor SR-BI were determined by flow cytometry. Internalization of HDL protein was demonstrated by confocal microscopy using HDL labeled with Alexa Fluor 488. HUVECs were used as reference to compared the characteristics with HMEC-1. Results HMEC-1 and HUVEC had similar morphologies, size and granularity. HMEC-1 expressed endothelial markers as HUVECs, as well as functional SR-B1 receptor since the cell line was able to internalize HDL particles. HMEC-1 effectively increased ICAM-1 and VCAM-1 expression after TNF-α stimulation. HUVECs showed more sensibility to TNF-α stimulus but the range of ICAM-1 and VCAM-1 expression was less homogeneous than in HMEC-1, probably due to biological variation of the former. Finally, the expression of adhesion molecules in HMEC-1 was attenuated by co-incubation with HDL. Conclusion HMEC-1 possess characteristics of endothelial cells, similar to HUVECs, being a cell line suitable to evaluate the functionality of HDL vis-à-vis the endothelium.http://link.springer.com/article/10.1186/s12944-018-0695-7HDLHUVECAdhesion moleculesInflammationEndoglinAtherosclerosis |
| spellingShingle | Mónica Muñoz-Vega Felipe Massó Araceli Páez Elizabeth Carreón-Torres Hector A. Cabrera-Fuentes José Manuel Fragoso Nonanzit Pérez-Hernández Laurent O. Martinez Souad Najib Gilberto Vargas-Alarcón Óscar Pérez-Méndez Characterization of immortalized human dermal microvascular endothelial cells (HMEC-1) for the study of HDL functionality Lipids in Health and Disease HDL HUVEC Adhesion molecules Inflammation Endoglin Atherosclerosis |
| title | Characterization of immortalized human dermal microvascular endothelial cells (HMEC-1) for the study of HDL functionality |
| title_full | Characterization of immortalized human dermal microvascular endothelial cells (HMEC-1) for the study of HDL functionality |
| title_fullStr | Characterization of immortalized human dermal microvascular endothelial cells (HMEC-1) for the study of HDL functionality |
| title_full_unstemmed | Characterization of immortalized human dermal microvascular endothelial cells (HMEC-1) for the study of HDL functionality |
| title_short | Characterization of immortalized human dermal microvascular endothelial cells (HMEC-1) for the study of HDL functionality |
| title_sort | characterization of immortalized human dermal microvascular endothelial cells hmec 1 for the study of hdl functionality |
| topic | HDL HUVEC Adhesion molecules Inflammation Endoglin Atherosclerosis |
| url | http://link.springer.com/article/10.1186/s12944-018-0695-7 |
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