Cytoprotective Role of Edible Seahorse (<i>Hippocampus abdominalis</i>)-Derived Peptides in H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress in Human Umbilical Vein Endothelial Cells
Oxidative stress-induced endothelial dysfunction is strongly linked to the pathogenesis of cardiovascular diseases. A previous study revealed that seahorse hydrolysates ameliorated oxidative stress-mediated human umbilical vein endothelial cells (HUVECs) injury. However, the responsible compounds ha...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-02-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/19/2/86 |
| _version_ | 1797415917569703936 |
|---|---|
| author | Yunok Oh Chang-Bum Ahn Jae-Young Je |
| author_facet | Yunok Oh Chang-Bum Ahn Jae-Young Je |
| author_sort | Yunok Oh |
| collection | DOAJ |
| description | Oxidative stress-induced endothelial dysfunction is strongly linked to the pathogenesis of cardiovascular diseases. A previous study revealed that seahorse hydrolysates ameliorated oxidative stress-mediated human umbilical vein endothelial cells (HUVECs) injury. However, the responsible compounds have not yet been identified. This study aimed to identify cytoprotective peptides and to investigate the molecular mechanism underlying the cytoprotective role in H<sub>2</sub>O<sub>2</sub>-induced HUVECs injury. After purification by gel filtration and HPLC, two peptides were sequenced by liquid chromatography-tandem mass spectrometry as HGSH (436.43 Da) and KGPSW (573.65 Da). The synthesized peptides and their combination (1:1 ratio) showed significant HUVECs protection effect at 100 μg/mL against H<sub>2</sub>O<sub>2</sub>-induced oxidative damage via significantly reducing intracellular reactive oxygen species (ROS). Two peptides and their combination treatment resulted in the increased heme oxygenase-1 (HO-1), a phase II detoxifying enzyme, through the activation of nuclear transcription factor-erythroid 2-related factor (Nrf2). Additionally, cell cycle and nuclear staining analysis revealed that two peptides and their combination significantly protected H<sub>2</sub>O<sub>2</sub>-induced cell death through antiapoptotic action. Two peptides and their combination treatment led to inhibit the expression of proapoptotic Bax, the release of cytochrome C into the cytosol, the activation of caspase 3 by H<sub>2</sub>O<sub>2</sub> treatment in HUVECs, whereas antiapoptotic Bcl-2 expression was increased with concomitant downregulation of Bax/Bcl-2 ratio. Taken together, these results suggest that seahorse-derived peptides may be a promising agent for oxidative stress-related cardiovascular diseases. |
| first_indexed | 2024-03-09T05:56:22Z |
| format | Article |
| id | doaj.art-8a800590fd634171a4ea6f320ac7140f |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T05:56:22Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-8a800590fd634171a4ea6f320ac7140f2023-12-03T12:13:35ZengMDPI AGMarine Drugs1660-33972021-02-011928610.3390/md19020086Cytoprotective Role of Edible Seahorse (<i>Hippocampus abdominalis</i>)-Derived Peptides in H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress in Human Umbilical Vein Endothelial CellsYunok Oh0Chang-Bum Ahn1Jae-Young Je2Institute of Marine Life Science, Pukyong National University, Busan 48513, KoreaDivision of Food and Nutrition, Chonnam National University, Gwangju 61186, KoreaDepartment of Marine-Bio Convergence Science, Pukyong National University, Busan 48547, KoreaOxidative stress-induced endothelial dysfunction is strongly linked to the pathogenesis of cardiovascular diseases. A previous study revealed that seahorse hydrolysates ameliorated oxidative stress-mediated human umbilical vein endothelial cells (HUVECs) injury. However, the responsible compounds have not yet been identified. This study aimed to identify cytoprotective peptides and to investigate the molecular mechanism underlying the cytoprotective role in H<sub>2</sub>O<sub>2</sub>-induced HUVECs injury. After purification by gel filtration and HPLC, two peptides were sequenced by liquid chromatography-tandem mass spectrometry as HGSH (436.43 Da) and KGPSW (573.65 Da). The synthesized peptides and their combination (1:1 ratio) showed significant HUVECs protection effect at 100 μg/mL against H<sub>2</sub>O<sub>2</sub>-induced oxidative damage via significantly reducing intracellular reactive oxygen species (ROS). Two peptides and their combination treatment resulted in the increased heme oxygenase-1 (HO-1), a phase II detoxifying enzyme, through the activation of nuclear transcription factor-erythroid 2-related factor (Nrf2). Additionally, cell cycle and nuclear staining analysis revealed that two peptides and their combination significantly protected H<sub>2</sub>O<sub>2</sub>-induced cell death through antiapoptotic action. Two peptides and their combination treatment led to inhibit the expression of proapoptotic Bax, the release of cytochrome C into the cytosol, the activation of caspase 3 by H<sub>2</sub>O<sub>2</sub> treatment in HUVECs, whereas antiapoptotic Bcl-2 expression was increased with concomitant downregulation of Bax/Bcl-2 ratio. Taken together, these results suggest that seahorse-derived peptides may be a promising agent for oxidative stress-related cardiovascular diseases.https://www.mdpi.com/1660-3397/19/2/86seahorsecytoprotective peptidesoxidative stressHO-1Nrf2antiapoptosis |
| spellingShingle | Yunok Oh Chang-Bum Ahn Jae-Young Je Cytoprotective Role of Edible Seahorse (<i>Hippocampus abdominalis</i>)-Derived Peptides in H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress in Human Umbilical Vein Endothelial Cells Marine Drugs seahorse cytoprotective peptides oxidative stress HO-1 Nrf2 antiapoptosis |
| title | Cytoprotective Role of Edible Seahorse (<i>Hippocampus abdominalis</i>)-Derived Peptides in H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress in Human Umbilical Vein Endothelial Cells |
| title_full | Cytoprotective Role of Edible Seahorse (<i>Hippocampus abdominalis</i>)-Derived Peptides in H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress in Human Umbilical Vein Endothelial Cells |
| title_fullStr | Cytoprotective Role of Edible Seahorse (<i>Hippocampus abdominalis</i>)-Derived Peptides in H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress in Human Umbilical Vein Endothelial Cells |
| title_full_unstemmed | Cytoprotective Role of Edible Seahorse (<i>Hippocampus abdominalis</i>)-Derived Peptides in H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress in Human Umbilical Vein Endothelial Cells |
| title_short | Cytoprotective Role of Edible Seahorse (<i>Hippocampus abdominalis</i>)-Derived Peptides in H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress in Human Umbilical Vein Endothelial Cells |
| title_sort | cytoprotective role of edible seahorse i hippocampus abdominalis i derived peptides in h sub 2 sub o sub 2 sub induced oxidative stress in human umbilical vein endothelial cells |
| topic | seahorse cytoprotective peptides oxidative stress HO-1 Nrf2 antiapoptosis |
| url | https://www.mdpi.com/1660-3397/19/2/86 |
| work_keys_str_mv | AT yunokoh cytoprotectiveroleofedibleseahorseihippocampusabdominalisiderivedpeptidesinhsub2subosub2subinducedoxidativestressinhumanumbilicalveinendothelialcells AT changbumahn cytoprotectiveroleofedibleseahorseihippocampusabdominalisiderivedpeptidesinhsub2subosub2subinducedoxidativestressinhumanumbilicalveinendothelialcells AT jaeyoungje cytoprotectiveroleofedibleseahorseihippocampusabdominalisiderivedpeptidesinhsub2subosub2subinducedoxidativestressinhumanumbilicalveinendothelialcells |