Identification of Diverse Integron and Plasmid Structures Carrying a Novel Carbapenemase Among Pseudomonas Species
A novel carbapenem-hydrolyzing beta-lactamase, called IMP-63, was identified in three clonally distinct strains of Pseudomonas aeruginosa and two strains of Pseudomonas putida isolated within a 4 year timeframe in three French hospitals. The blaIMP–63 gene that encodes this carbapenemase turned out...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.00404/full |
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| author | Eleni Liapis Eleni Liapis Maxime Bour Pauline Triponney Thomas Jové Jean-Ralph Zahar Benoît Valot Katy Jeannot Katy Jeannot Patrick Plésiat Patrick Plésiat |
| author_facet | Eleni Liapis Eleni Liapis Maxime Bour Pauline Triponney Thomas Jové Jean-Ralph Zahar Benoît Valot Katy Jeannot Katy Jeannot Patrick Plésiat Patrick Plésiat |
| author_sort | Eleni Liapis |
| collection | DOAJ |
| description | A novel carbapenem-hydrolyzing beta-lactamase, called IMP-63, was identified in three clonally distinct strains of Pseudomonas aeruginosa and two strains of Pseudomonas putida isolated within a 4 year timeframe in three French hospitals. The blaIMP–63 gene that encodes this carbapenemase turned out to be located in the variable region of four integrons (In1297, In1574, In1573, and In1572) and to coexist with novel or rare gene cassettes (fosM, gcu170, gcuF1) and insertion elements (ISPsp7v, ISPa16v). All these integrons except one (In1574) were flanked by a copy of insertion sequence ISPa17 next to the orf6 putative gene, and were carried by non-conjugative plasmids (pNECK1, pROUSS1, pROUSS2, pROUE1). These plasmids exhibit unique modular structures and partial sequence homologies with plasmids previously identified in various non-fermenting environmental Gram-negative species. Lines of evidence suggest that ISPa17 promoted en bloc the transposition of IMP-63-encoding integrons on these different plasmids. As demonstrated by genotyping experiments, isolates of P. aeruginosa harboring the 28.9-kb plasmid pNECK1 and belonging to international “high-risk” clone ST308 were responsible for an outbreak in one hospital. Collectively, these data provide an insight into the complex and unpredictable routes of diffusion of some resistance determinants, here blaIMP–63, among Pseudomonas species. |
| first_indexed | 2024-12-16T09:51:28Z |
| format | Article |
| id | doaj.art-8a81addb02e24381ae810a7ab578e048 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-16T09:51:28Z |
| publishDate | 2019-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-8a81addb02e24381ae810a7ab578e0482022-12-21T22:36:02ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-03-011010.3389/fmicb.2019.00404433205Identification of Diverse Integron and Plasmid Structures Carrying a Novel Carbapenemase Among Pseudomonas SpeciesEleni Liapis0Eleni Liapis1Maxime Bour2Pauline Triponney3Thomas Jové4Jean-Ralph Zahar5Benoît Valot6Katy Jeannot7Katy Jeannot8Patrick Plésiat9Patrick Plésiat10Centre National de Référence de la Résistance aux Antibiotiques, CHRU Jean Minjoz, Besançon, FranceCNRS, UMR 6249 Chrono-Environnement, Besançon, FranceCentre National de Référence de la Résistance aux Antibiotiques, CHRU Jean Minjoz, Besançon, FranceCentre National de Référence de la Résistance aux Antibiotiques, CHRU Jean Minjoz, Besançon, FranceINSERM UMR 1092, Université de Limoges, CHU Limoges, Limoges, FranceDépartement de Microbiologie Clinique, AP-HP, CHU Avicenne, Bobigny, FranceCNRS, UMR 6249 Chrono-Environnement, Besançon, FranceCentre National de Référence de la Résistance aux Antibiotiques, CHRU Jean Minjoz, Besançon, FranceCNRS, UMR 6249 Chrono-Environnement, Besançon, FranceCentre National de Référence de la Résistance aux Antibiotiques, CHRU Jean Minjoz, Besançon, FranceCNRS, UMR 6249 Chrono-Environnement, Besançon, FranceA novel carbapenem-hydrolyzing beta-lactamase, called IMP-63, was identified in three clonally distinct strains of Pseudomonas aeruginosa and two strains of Pseudomonas putida isolated within a 4 year timeframe in three French hospitals. The blaIMP–63 gene that encodes this carbapenemase turned out to be located in the variable region of four integrons (In1297, In1574, In1573, and In1572) and to coexist with novel or rare gene cassettes (fosM, gcu170, gcuF1) and insertion elements (ISPsp7v, ISPa16v). All these integrons except one (In1574) were flanked by a copy of insertion sequence ISPa17 next to the orf6 putative gene, and were carried by non-conjugative plasmids (pNECK1, pROUSS1, pROUSS2, pROUE1). These plasmids exhibit unique modular structures and partial sequence homologies with plasmids previously identified in various non-fermenting environmental Gram-negative species. Lines of evidence suggest that ISPa17 promoted en bloc the transposition of IMP-63-encoding integrons on these different plasmids. As demonstrated by genotyping experiments, isolates of P. aeruginosa harboring the 28.9-kb plasmid pNECK1 and belonging to international “high-risk” clone ST308 were responsible for an outbreak in one hospital. Collectively, these data provide an insight into the complex and unpredictable routes of diffusion of some resistance determinants, here blaIMP–63, among Pseudomonas species.https://www.frontiersin.org/article/10.3389/fmicb.2019.00404/fullPseudomonasIMP carbapenemasesplasmidsintegronsdiffusion |
| spellingShingle | Eleni Liapis Eleni Liapis Maxime Bour Pauline Triponney Thomas Jové Jean-Ralph Zahar Benoît Valot Katy Jeannot Katy Jeannot Patrick Plésiat Patrick Plésiat Identification of Diverse Integron and Plasmid Structures Carrying a Novel Carbapenemase Among Pseudomonas Species Frontiers in Microbiology Pseudomonas IMP carbapenemases plasmids integrons diffusion |
| title | Identification of Diverse Integron and Plasmid Structures Carrying a Novel Carbapenemase Among Pseudomonas Species |
| title_full | Identification of Diverse Integron and Plasmid Structures Carrying a Novel Carbapenemase Among Pseudomonas Species |
| title_fullStr | Identification of Diverse Integron and Plasmid Structures Carrying a Novel Carbapenemase Among Pseudomonas Species |
| title_full_unstemmed | Identification of Diverse Integron and Plasmid Structures Carrying a Novel Carbapenemase Among Pseudomonas Species |
| title_short | Identification of Diverse Integron and Plasmid Structures Carrying a Novel Carbapenemase Among Pseudomonas Species |
| title_sort | identification of diverse integron and plasmid structures carrying a novel carbapenemase among pseudomonas species |
| topic | Pseudomonas IMP carbapenemases plasmids integrons diffusion |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.00404/full |
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