Parameter estimation of the structured illumination pattern based on principal component analysis (PCA): PCA-SIM

Abstract Principal component analysis (PCA), a common dimensionality reduction method, is introduced into SIM to identify the frequency vectors and pattern phases of the illumination pattern with precise subpixel accuracy, fast speed, and noise-robustness, which is promising for real-time and long-t...

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Main Authors: Xin Chen, Yiwei Hou, Peng Xi
Format: Article
Language:English
Published: Nature Publishing Group 2023-02-01
Series:Light: Science & Applications
Online Access:https://doi.org/10.1038/s41377-022-01043-9
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author Xin Chen
Yiwei Hou
Peng Xi
author_facet Xin Chen
Yiwei Hou
Peng Xi
author_sort Xin Chen
collection DOAJ
description Abstract Principal component analysis (PCA), a common dimensionality reduction method, is introduced into SIM to identify the frequency vectors and pattern phases of the illumination pattern with precise subpixel accuracy, fast speed, and noise-robustness, which is promising for real-time and long-term live-cell imaging.
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spelling doaj.art-8a81e67b0a0e411e9a153510ff3bcf002023-02-12T12:23:12ZengNature Publishing GroupLight: Science & Applications2047-75382023-02-011211310.1038/s41377-022-01043-9Parameter estimation of the structured illumination pattern based on principal component analysis (PCA): PCA-SIMXin Chen0Yiwei Hou1Peng Xi2Department of Biomedical Engineering, College of Future Technology, Peking UniversityDepartment of Biomedical Engineering, College of Future Technology, Peking UniversityDepartment of Biomedical Engineering, College of Future Technology, Peking UniversityAbstract Principal component analysis (PCA), a common dimensionality reduction method, is introduced into SIM to identify the frequency vectors and pattern phases of the illumination pattern with precise subpixel accuracy, fast speed, and noise-robustness, which is promising for real-time and long-term live-cell imaging.https://doi.org/10.1038/s41377-022-01043-9
spellingShingle Xin Chen
Yiwei Hou
Peng Xi
Parameter estimation of the structured illumination pattern based on principal component analysis (PCA): PCA-SIM
Light: Science & Applications
title Parameter estimation of the structured illumination pattern based on principal component analysis (PCA): PCA-SIM
title_full Parameter estimation of the structured illumination pattern based on principal component analysis (PCA): PCA-SIM
title_fullStr Parameter estimation of the structured illumination pattern based on principal component analysis (PCA): PCA-SIM
title_full_unstemmed Parameter estimation of the structured illumination pattern based on principal component analysis (PCA): PCA-SIM
title_short Parameter estimation of the structured illumination pattern based on principal component analysis (PCA): PCA-SIM
title_sort parameter estimation of the structured illumination pattern based on principal component analysis pca pca sim
url https://doi.org/10.1038/s41377-022-01043-9
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AT pengxi parameterestimationofthestructuredilluminationpatternbasedonprincipalcomponentanalysispcapcasim