Investigation of integrated time nanosecond pulse irreversible electroporation against spontaneous equine melanoma
IntroductionIntegrated time nanosecond pulse irreversible electroporation (INSPIRE) is a novel tumor ablation modality that employs high voltage, alternating polarity waveforms to induce cell death in a well-defined volume while sparing the underlying tissue. This study aimed to demonstrate the in v...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1232650/full |
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| author | Chris C. Fesmire Bridgette Peal Jennifer Ruff Elizabeth Moyer Thomas J. McParland Kobi Derks Erin O’Neil Carrie Emke Brianna Johnson Shatorupa Ghosh Ross A. Petrella Matthew R. DeWitt Timo Prange Callie Fogle Michael B. Sano Michael B. Sano |
| author_facet | Chris C. Fesmire Bridgette Peal Jennifer Ruff Elizabeth Moyer Thomas J. McParland Kobi Derks Erin O’Neil Carrie Emke Brianna Johnson Shatorupa Ghosh Ross A. Petrella Matthew R. DeWitt Timo Prange Callie Fogle Michael B. Sano Michael B. Sano |
| author_sort | Chris C. Fesmire |
| collection | DOAJ |
| description | IntroductionIntegrated time nanosecond pulse irreversible electroporation (INSPIRE) is a novel tumor ablation modality that employs high voltage, alternating polarity waveforms to induce cell death in a well-defined volume while sparing the underlying tissue. This study aimed to demonstrate the in vivo efficacy of INSPIRE against spontaneous melanoma in standing, awake horses.MethodsA custom applicator and a pulse generation system were utilized in a pilot study to treat horses presenting with spontaneous melanoma. INSPIRE treatments were administered to 32 tumors across 6 horses and an additional 13 tumors were followed to act as untreated controls. Tumors were tracked over a 43–85 day period following a single INSPIRE treatment. Pulse widths of 500ns and 2000ns with voltages between 1000 V and 2000 V were investigated to determine the effect of these variables on treatment outcomes.ResultsTreatments administered at the lowest voltage (1000 V) reduced tumor volumes by 11 to 15%. Higher voltage (2000 V) treatments reduced tumor volumes by 84 to 88% and eliminated 33% and 80% of tumors when 500 ns and 2000 ns pulses were administered, respectively.DiscussionPromising results were achieved without the use of chemotherapeutics, the use of general anesthesia, or the need for surgical resection in regions which are challenging to keep sterile. This novel therapeutic approach has the potential to expand the role of pulsed electric fields in veterinary patients, especially when general anesthesia is contraindicated, and warrants future studies to demonstrate the efficacy of INSPIRE as a solid tumor treatment. |
| first_indexed | 2024-03-08T09:41:54Z |
| format | Article |
| id | doaj.art-8a839b0513b54072a7eafa1804829e78 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-08T09:41:54Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-8a839b0513b54072a7eafa1804829e782024-01-30T04:23:00ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692024-01-011110.3389/fvets.2024.12326501232650Investigation of integrated time nanosecond pulse irreversible electroporation against spontaneous equine melanomaChris C. Fesmire0Bridgette Peal1Jennifer Ruff2Elizabeth Moyer3Thomas J. McParland4Kobi Derks5Erin O’Neil6Carrie Emke7Brianna Johnson8Shatorupa Ghosh9Ross A. Petrella10Matthew R. DeWitt11Timo Prange12Callie Fogle13Michael B. Sano14Michael B. Sano15Bioelectricity Lab, UNC/NCSU Joint Department of Biomedical Engineering, Raleigh, NC, United StatesDepartment of Clinical Sciences, NC State College of Veterinary Medicine, Raleigh, NC, United StatesDepartment of Clinical Sciences, NC State College of Veterinary Medicine, Raleigh, NC, United StatesDepartment of Clinical Sciences, NC State College of Veterinary Medicine, Raleigh, NC, United StatesDepartment of Clinical Sciences, NC State College of Veterinary Medicine, Raleigh, NC, United StatesDepartment of Clinical Sciences, NC State College of Veterinary Medicine, Raleigh, NC, United StatesDepartment of Clinical Sciences, NC State College of Veterinary Medicine, Raleigh, NC, United StatesClinical Studies Core, NC State College of Veterinary Medicine, Raleigh, NC, United StatesClinical Studies Core, NC State College of Veterinary Medicine, Raleigh, NC, United StatesBioelectricity Lab, UNC/NCSU Joint Department of Biomedical Engineering, Raleigh, NC, United StatesBioelectricity Lab, UNC/NCSU Joint Department of Biomedical Engineering, Raleigh, NC, United StatesBioelectricity Lab, UNC/NCSU Joint Department of Biomedical Engineering, Raleigh, NC, United StatesDepartment of Clinical Sciences, NC State College of Veterinary Medicine, Raleigh, NC, United StatesDepartment of Clinical Sciences, NC State College of Veterinary Medicine, Raleigh, NC, United StatesBioelectricity Lab, UNC/NCSU Joint Department of Biomedical Engineering, Raleigh, NC, United StatesDepartment of Molecular Biomedical Sciences, NC State College of Veterinary Medicine, Raleigh, NC, United StatesIntroductionIntegrated time nanosecond pulse irreversible electroporation (INSPIRE) is a novel tumor ablation modality that employs high voltage, alternating polarity waveforms to induce cell death in a well-defined volume while sparing the underlying tissue. This study aimed to demonstrate the in vivo efficacy of INSPIRE against spontaneous melanoma in standing, awake horses.MethodsA custom applicator and a pulse generation system were utilized in a pilot study to treat horses presenting with spontaneous melanoma. INSPIRE treatments were administered to 32 tumors across 6 horses and an additional 13 tumors were followed to act as untreated controls. Tumors were tracked over a 43–85 day period following a single INSPIRE treatment. Pulse widths of 500ns and 2000ns with voltages between 1000 V and 2000 V were investigated to determine the effect of these variables on treatment outcomes.ResultsTreatments administered at the lowest voltage (1000 V) reduced tumor volumes by 11 to 15%. Higher voltage (2000 V) treatments reduced tumor volumes by 84 to 88% and eliminated 33% and 80% of tumors when 500 ns and 2000 ns pulses were administered, respectively.DiscussionPromising results were achieved without the use of chemotherapeutics, the use of general anesthesia, or the need for surgical resection in regions which are challenging to keep sterile. This novel therapeutic approach has the potential to expand the role of pulsed electric fields in veterinary patients, especially when general anesthesia is contraindicated, and warrants future studies to demonstrate the efficacy of INSPIRE as a solid tumor treatment.https://www.frontiersin.org/articles/10.3389/fvets.2024.1232650/fullfocal ablationpulsed field ablationINSPIREskin cancerclinical trial |
| spellingShingle | Chris C. Fesmire Bridgette Peal Jennifer Ruff Elizabeth Moyer Thomas J. McParland Kobi Derks Erin O’Neil Carrie Emke Brianna Johnson Shatorupa Ghosh Ross A. Petrella Matthew R. DeWitt Timo Prange Callie Fogle Michael B. Sano Michael B. Sano Investigation of integrated time nanosecond pulse irreversible electroporation against spontaneous equine melanoma Frontiers in Veterinary Science focal ablation pulsed field ablation INSPIRE skin cancer clinical trial |
| title | Investigation of integrated time nanosecond pulse irreversible electroporation against spontaneous equine melanoma |
| title_full | Investigation of integrated time nanosecond pulse irreversible electroporation against spontaneous equine melanoma |
| title_fullStr | Investigation of integrated time nanosecond pulse irreversible electroporation against spontaneous equine melanoma |
| title_full_unstemmed | Investigation of integrated time nanosecond pulse irreversible electroporation against spontaneous equine melanoma |
| title_short | Investigation of integrated time nanosecond pulse irreversible electroporation against spontaneous equine melanoma |
| title_sort | investigation of integrated time nanosecond pulse irreversible electroporation against spontaneous equine melanoma |
| topic | focal ablation pulsed field ablation INSPIRE skin cancer clinical trial |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1232650/full |
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