Analysis of the virulence, infection process, and extracellular enzyme activities of Aspergillus nomius against the Asian corn borer, Ostrinia furnacalis guenée (Lepidoptera: Crambidae)
ABSTRACTThe control of Ostrinia furnacalis, a major pest of maize in Xinjiang, is challenging owing to the occurrence of resistant individuals. Entomopathogenic fungi (EPF) are natural insect regulators used as substitutes for synthetic chemical insecticides. The fungus Aspergillus nomius is highly...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Virulence |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21505594.2023.2265108 |
| _version_ | 1797367132424503296 |
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| author | Xiaowu Wang Xinhua Ding Zihan Yuan Zunzun Jia Kaiyun Fu Faqiang Zhan Wenchao Guo Liuyan Zhou Haiqiang Li Jinping Dai Zhifang Wang Yuqing Xie Xinping Yang |
| author_facet | Xiaowu Wang Xinhua Ding Zihan Yuan Zunzun Jia Kaiyun Fu Faqiang Zhan Wenchao Guo Liuyan Zhou Haiqiang Li Jinping Dai Zhifang Wang Yuqing Xie Xinping Yang |
| author_sort | Xiaowu Wang |
| collection | DOAJ |
| description | ABSTRACTThe control of Ostrinia furnacalis, a major pest of maize in Xinjiang, is challenging owing to the occurrence of resistant individuals. Entomopathogenic fungi (EPF) are natural insect regulators used as substitutes for synthetic chemical insecticides. The fungus Aspergillus nomius is highly pathogenic to O. furnacalis; however, its virulence characteristics have not been identified. This study aimed to analyse the lethal efficacy, mode of infection on the cuticle, and extracellular enzyme activity of A. nomius against O. furnacalis. We found that the mortality and mycosis of O. furnacalis were dose-dependent when exposed to A. nomius and varied at different life stages. The egg-hatching and adult emergence rates decreased with an increase in conidial suspension. The highest mortality (83.33%, 7 d post-infection [DPI]) and mycosis (74.33%, 7 DPI) and the lowest mortality response (8.52 × 103 conidia mL−1) and median lethal time (4.91 d) occurred in the 3rd instar larvae of O. furnacalis. Scanning electron microscopy indicated that numerous conidia germination and infection structure formation may have contributed to the high pathogenicity of A. nomius against O. furnacalis. There were significant correlations between O. furnacalis mortality and the activities of extracellular protease, lipase, and chitinase of A. nomius. This study revealed the infection process of the highly pathogenic A. nomius against O. furnacalis, providing a theoretical basis and reference for strain improvement and field application of EPF. |
| first_indexed | 2024-03-08T17:13:59Z |
| format | Article |
| id | doaj.art-8a83c3641ff84d6f98b28a6cb5dea22c |
| institution | Directory Open Access Journal |
| issn | 2150-5594 2150-5608 |
| language | English |
| last_indexed | 2024-03-08T17:13:59Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj.art-8a83c3641ff84d6f98b28a6cb5dea22c2024-01-03T17:26:57ZengTaylor & Francis GroupVirulence2150-55942150-56082023-12-0114110.1080/21505594.2023.2265108Analysis of the virulence, infection process, and extracellular enzyme activities of Aspergillus nomius against the Asian corn borer, Ostrinia furnacalis guenée (Lepidoptera: Crambidae)Xiaowu Wang0Xinhua Ding1Zihan Yuan2Zunzun Jia3Kaiyun Fu4Faqiang Zhan5Wenchao Guo6Liuyan Zhou7Haiqiang Li8Jinping Dai9Zhifang Wang10Yuqing Xie11Xinping Yang12Institute of Microbiology Applications, Xinjiang Academy of Agricultural Sciences, Ürümqi, PR ChinaKey Laboratory of Integrated Pest Management on Crops in Northwestern Oasis, Ministry of Agriculture, Ürümqi, PR ChinaInstitute of Microbiology Applications, Xinjiang Academy of Agricultural Sciences, Ürümqi, PR ChinaKey Laboratory of Integrated Pest Management on Crops in Northwestern Oasis, Ministry of Agriculture, Ürümqi, PR ChinaKey Laboratory of Integrated Pest Management on Crops in Northwestern Oasis, Ministry of Agriculture, Ürümqi, PR ChinaInstitute of Microbiology Applications, Xinjiang Academy of Agricultural Sciences, Ürümqi, PR ChinaKey Laboratory of Integrated Pest Management on Crops in Northwestern Oasis, Ministry of Agriculture, Ürümqi, PR ChinaInstitute of Microbiology Applications, Xinjiang Academy of Agricultural Sciences, Ürümqi, PR ChinaKey Laboratory of Integrated Pest Management on Crops in Northwestern Oasis, Ministry of Agriculture, Ürümqi, PR ChinaInstitute of Microbiology Applications, Xinjiang Academy of Agricultural Sciences, Ürümqi, PR ChinaInstitute of Microbiology Applications, Xinjiang Academy of Agricultural Sciences, Ürümqi, PR ChinaInstitute of Microbiology Applications, Xinjiang Academy of Agricultural Sciences, Ürümqi, PR ChinaInstitute of Microbiology Applications, Xinjiang Academy of Agricultural Sciences, Ürümqi, PR ChinaABSTRACTThe control of Ostrinia furnacalis, a major pest of maize in Xinjiang, is challenging owing to the occurrence of resistant individuals. Entomopathogenic fungi (EPF) are natural insect regulators used as substitutes for synthetic chemical insecticides. The fungus Aspergillus nomius is highly pathogenic to O. furnacalis; however, its virulence characteristics have not been identified. This study aimed to analyse the lethal efficacy, mode of infection on the cuticle, and extracellular enzyme activity of A. nomius against O. furnacalis. We found that the mortality and mycosis of O. furnacalis were dose-dependent when exposed to A. nomius and varied at different life stages. The egg-hatching and adult emergence rates decreased with an increase in conidial suspension. The highest mortality (83.33%, 7 d post-infection [DPI]) and mycosis (74.33%, 7 DPI) and the lowest mortality response (8.52 × 103 conidia mL−1) and median lethal time (4.91 d) occurred in the 3rd instar larvae of O. furnacalis. Scanning electron microscopy indicated that numerous conidia germination and infection structure formation may have contributed to the high pathogenicity of A. nomius against O. furnacalis. There were significant correlations between O. furnacalis mortality and the activities of extracellular protease, lipase, and chitinase of A. nomius. This study revealed the infection process of the highly pathogenic A. nomius against O. furnacalis, providing a theoretical basis and reference for strain improvement and field application of EPF.https://www.tandfonline.com/doi/10.1080/21505594.2023.2265108Aspergillus nomiusostrinia furnacalislethal efficacyinfection processescuticleextracellular enzymes activity |
| spellingShingle | Xiaowu Wang Xinhua Ding Zihan Yuan Zunzun Jia Kaiyun Fu Faqiang Zhan Wenchao Guo Liuyan Zhou Haiqiang Li Jinping Dai Zhifang Wang Yuqing Xie Xinping Yang Analysis of the virulence, infection process, and extracellular enzyme activities of Aspergillus nomius against the Asian corn borer, Ostrinia furnacalis guenée (Lepidoptera: Crambidae) Virulence Aspergillus nomius ostrinia furnacalis lethal efficacy infection processes cuticle extracellular enzymes activity |
| title | Analysis of the virulence, infection process, and extracellular enzyme activities of Aspergillus nomius against the Asian corn borer, Ostrinia furnacalis guenée (Lepidoptera: Crambidae) |
| title_full | Analysis of the virulence, infection process, and extracellular enzyme activities of Aspergillus nomius against the Asian corn borer, Ostrinia furnacalis guenée (Lepidoptera: Crambidae) |
| title_fullStr | Analysis of the virulence, infection process, and extracellular enzyme activities of Aspergillus nomius against the Asian corn borer, Ostrinia furnacalis guenée (Lepidoptera: Crambidae) |
| title_full_unstemmed | Analysis of the virulence, infection process, and extracellular enzyme activities of Aspergillus nomius against the Asian corn borer, Ostrinia furnacalis guenée (Lepidoptera: Crambidae) |
| title_short | Analysis of the virulence, infection process, and extracellular enzyme activities of Aspergillus nomius against the Asian corn borer, Ostrinia furnacalis guenée (Lepidoptera: Crambidae) |
| title_sort | analysis of the virulence infection process and extracellular enzyme activities of aspergillus nomius against the asian corn borer ostrinia furnacalis guenee lepidoptera crambidae |
| topic | Aspergillus nomius ostrinia furnacalis lethal efficacy infection processes cuticle extracellular enzymes activity |
| url | https://www.tandfonline.com/doi/10.1080/21505594.2023.2265108 |
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