Fatty Acid Metabolite Profiling Reveals Oxylipins as Markers of Brown but Not Brite Adipose Tissue
Metabolites of omega-6 and omega-3 polyunsaturated fatty acids are important signaling molecules implicated in the control of adipogenesis and energy balance regulation. Some of these metabolites belonging to the group of oxylipins have been associated with non-shivering thermogenesis in mice mediat...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-02-01
|
| Series: | Frontiers in Endocrinology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fendo.2020.00073/full |
| _version_ | 1818032768431423488 |
|---|---|
| author | Sebastian Dieckmann Sebastian Dieckmann Sebastian Dieckmann Stefanie Maurer Stefanie Maurer Stefanie Maurer Tobias Fromme Tobias Fromme Tobias Fromme Cécilia Colson Kirsi A. Virtanen Ez-Zoubir Amri Martin Klingenspor Martin Klingenspor Martin Klingenspor |
| author_facet | Sebastian Dieckmann Sebastian Dieckmann Sebastian Dieckmann Stefanie Maurer Stefanie Maurer Stefanie Maurer Tobias Fromme Tobias Fromme Tobias Fromme Cécilia Colson Kirsi A. Virtanen Ez-Zoubir Amri Martin Klingenspor Martin Klingenspor Martin Klingenspor |
| author_sort | Sebastian Dieckmann |
| collection | DOAJ |
| description | Metabolites of omega-6 and omega-3 polyunsaturated fatty acids are important signaling molecules implicated in the control of adipogenesis and energy balance regulation. Some of these metabolites belonging to the group of oxylipins have been associated with non-shivering thermogenesis in mice mediated by brown or brite adipose tissue. We aimed to identify novel molecules with thermogenic potential and to clarify the relevance of these findings in a translational context. Therefore, we characterized and compared the oxylipin profiles of murine and human adipose tissues with different abundance of brown or brite adipocytes. A broad panel of 36 fatty acid metabolites was quantified in brown and white adipose tissues of C57BL/6J mice acclimatized to different ambient temperatures and in biopsies of human supraclavicular brown and white adipose tissue. The oxylipin profile of murine brite adipose tissue was not distinguishable from white adipose tissue, suggesting that adipose tissue browning in vivo is not associated with major changes in the oxylipin metabolism. Human brown and white adipose tissue also exhibited similar metabolite profiles. This is in line with previous studies proposing human brown adipose tissue to resemble the nature of murine brite adipose tissue representing a heterogeneous mixture of brite and white adipocytes. Although the global oxylipin profile served as a marker for the abundance of thermogenic adipocytes in bona fide brown but not white adipose tissue, we identified 5-HETE and 5,6-EET as individual compounds consistently associated with the abundance of brown or brite adipocytes in human BAT and murine brite fat. Further studies need to establish whether these candidates are mere markers or functional effectors of thermogenic capacity. |
| first_indexed | 2024-12-10T06:12:37Z |
| format | Article |
| id | doaj.art-8a8436b4cd374aae957e554e24b7ef28 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-12-10T06:12:37Z |
| publishDate | 2020-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-8a8436b4cd374aae957e554e24b7ef282022-12-22T01:59:32ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922020-02-011110.3389/fendo.2020.00073515961Fatty Acid Metabolite Profiling Reveals Oxylipins as Markers of Brown but Not Brite Adipose TissueSebastian Dieckmann0Sebastian Dieckmann1Sebastian Dieckmann2Stefanie Maurer3Stefanie Maurer4Stefanie Maurer5Tobias Fromme6Tobias Fromme7Tobias Fromme8Cécilia Colson9Kirsi A. Virtanen10Ez-Zoubir Amri11Martin Klingenspor12Martin Klingenspor13Martin Klingenspor14Chair for Molecular Nutritional Medicine, TUM School of Life Sciences, Technical University of Munich, Freising, GermanyEKFZ - Else Kröner-Fresenius Center for Nutritional Medicine, Technical University of Munich, Freising, GermanyZIEL Institute for Food and Health, TUM School of Life Sciences, Technical University of Munich, Freising, GermanyChair for Molecular Nutritional Medicine, TUM School of Life Sciences, Technical University of Munich, Freising, GermanyEKFZ - Else Kröner-Fresenius Center for Nutritional Medicine, Technical University of Munich, Freising, GermanyZIEL Institute for Food and Health, TUM School of Life Sciences, Technical University of Munich, Freising, GermanyChair for Molecular Nutritional Medicine, TUM School of Life Sciences, Technical University of Munich, Freising, GermanyEKFZ - Else Kröner-Fresenius Center for Nutritional Medicine, Technical University of Munich, Freising, GermanyZIEL Institute for Food and Health, TUM School of Life Sciences, Technical University of Munich, Freising, GermanyUniversité Côte d'Azur, CNRS, Inserm, iBV, Nice, FranceTurku PET Centre, Turku University Hospital, University of Turku, Turku, FinlandUniversité Côte d'Azur, CNRS, Inserm, iBV, Nice, FranceChair for Molecular Nutritional Medicine, TUM School of Life Sciences, Technical University of Munich, Freising, GermanyEKFZ - Else Kröner-Fresenius Center for Nutritional Medicine, Technical University of Munich, Freising, GermanyZIEL Institute for Food and Health, TUM School of Life Sciences, Technical University of Munich, Freising, GermanyMetabolites of omega-6 and omega-3 polyunsaturated fatty acids are important signaling molecules implicated in the control of adipogenesis and energy balance regulation. Some of these metabolites belonging to the group of oxylipins have been associated with non-shivering thermogenesis in mice mediated by brown or brite adipose tissue. We aimed to identify novel molecules with thermogenic potential and to clarify the relevance of these findings in a translational context. Therefore, we characterized and compared the oxylipin profiles of murine and human adipose tissues with different abundance of brown or brite adipocytes. A broad panel of 36 fatty acid metabolites was quantified in brown and white adipose tissues of C57BL/6J mice acclimatized to different ambient temperatures and in biopsies of human supraclavicular brown and white adipose tissue. The oxylipin profile of murine brite adipose tissue was not distinguishable from white adipose tissue, suggesting that adipose tissue browning in vivo is not associated with major changes in the oxylipin metabolism. Human brown and white adipose tissue also exhibited similar metabolite profiles. This is in line with previous studies proposing human brown adipose tissue to resemble the nature of murine brite adipose tissue representing a heterogeneous mixture of brite and white adipocytes. Although the global oxylipin profile served as a marker for the abundance of thermogenic adipocytes in bona fide brown but not white adipose tissue, we identified 5-HETE and 5,6-EET as individual compounds consistently associated with the abundance of brown or brite adipocytes in human BAT and murine brite fat. Further studies need to establish whether these candidates are mere markers or functional effectors of thermogenic capacity.https://www.frontiersin.org/article/10.3389/fendo.2020.00073/fulladipose tissuebrowningthermogenesisPUFA (polyunsaturated fatty acid)n-6 fatty acidn-3 fatty acid |
| spellingShingle | Sebastian Dieckmann Sebastian Dieckmann Sebastian Dieckmann Stefanie Maurer Stefanie Maurer Stefanie Maurer Tobias Fromme Tobias Fromme Tobias Fromme Cécilia Colson Kirsi A. Virtanen Ez-Zoubir Amri Martin Klingenspor Martin Klingenspor Martin Klingenspor Fatty Acid Metabolite Profiling Reveals Oxylipins as Markers of Brown but Not Brite Adipose Tissue Frontiers in Endocrinology adipose tissue browning thermogenesis PUFA (polyunsaturated fatty acid) n-6 fatty acid n-3 fatty acid |
| title | Fatty Acid Metabolite Profiling Reveals Oxylipins as Markers of Brown but Not Brite Adipose Tissue |
| title_full | Fatty Acid Metabolite Profiling Reveals Oxylipins as Markers of Brown but Not Brite Adipose Tissue |
| title_fullStr | Fatty Acid Metabolite Profiling Reveals Oxylipins as Markers of Brown but Not Brite Adipose Tissue |
| title_full_unstemmed | Fatty Acid Metabolite Profiling Reveals Oxylipins as Markers of Brown but Not Brite Adipose Tissue |
| title_short | Fatty Acid Metabolite Profiling Reveals Oxylipins as Markers of Brown but Not Brite Adipose Tissue |
| title_sort | fatty acid metabolite profiling reveals oxylipins as markers of brown but not brite adipose tissue |
| topic | adipose tissue browning thermogenesis PUFA (polyunsaturated fatty acid) n-6 fatty acid n-3 fatty acid |
| url | https://www.frontiersin.org/article/10.3389/fendo.2020.00073/full |
| work_keys_str_mv | AT sebastiandieckmann fattyacidmetaboliteprofilingrevealsoxylipinsasmarkersofbrownbutnotbriteadiposetissue AT sebastiandieckmann fattyacidmetaboliteprofilingrevealsoxylipinsasmarkersofbrownbutnotbriteadiposetissue AT sebastiandieckmann fattyacidmetaboliteprofilingrevealsoxylipinsasmarkersofbrownbutnotbriteadiposetissue AT stefaniemaurer fattyacidmetaboliteprofilingrevealsoxylipinsasmarkersofbrownbutnotbriteadiposetissue AT stefaniemaurer fattyacidmetaboliteprofilingrevealsoxylipinsasmarkersofbrownbutnotbriteadiposetissue AT stefaniemaurer fattyacidmetaboliteprofilingrevealsoxylipinsasmarkersofbrownbutnotbriteadiposetissue AT tobiasfromme fattyacidmetaboliteprofilingrevealsoxylipinsasmarkersofbrownbutnotbriteadiposetissue AT tobiasfromme fattyacidmetaboliteprofilingrevealsoxylipinsasmarkersofbrownbutnotbriteadiposetissue AT tobiasfromme fattyacidmetaboliteprofilingrevealsoxylipinsasmarkersofbrownbutnotbriteadiposetissue AT ceciliacolson fattyacidmetaboliteprofilingrevealsoxylipinsasmarkersofbrownbutnotbriteadiposetissue AT kirsiavirtanen fattyacidmetaboliteprofilingrevealsoxylipinsasmarkersofbrownbutnotbriteadiposetissue AT ezzoubiramri fattyacidmetaboliteprofilingrevealsoxylipinsasmarkersofbrownbutnotbriteadiposetissue AT martinklingenspor fattyacidmetaboliteprofilingrevealsoxylipinsasmarkersofbrownbutnotbriteadiposetissue AT martinklingenspor fattyacidmetaboliteprofilingrevealsoxylipinsasmarkersofbrownbutnotbriteadiposetissue AT martinklingenspor fattyacidmetaboliteprofilingrevealsoxylipinsasmarkersofbrownbutnotbriteadiposetissue |