Paraquat induces different programmed cell death patterns in Microcystis aeruginosa and Chlorella luteoviridis
Although programmed cell death (PCD) has been reported in phytoplankton, knowledge of the characterization of the PCD pathway and cascade process in different phytoplankton species is still limited. In this study, PCD progression in cyanobacterium Microcystis aeruginosa and green algae Chlorella lut...
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| Language: | English |
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Elsevier
2023-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322012696 |
| _version_ | 1797961288719007744 |
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| author | Fang Bai Yunlu Jia Jie Li Zhongxing Wu Lin Li Lirong Song |
| author_facet | Fang Bai Yunlu Jia Jie Li Zhongxing Wu Lin Li Lirong Song |
| author_sort | Fang Bai |
| collection | DOAJ |
| description | Although programmed cell death (PCD) has been reported in phytoplankton, knowledge of the characterization of the PCD pathway and cascade process in different phytoplankton species is still limited. In this study, PCD progression in cyanobacterium Microcystis aeruginosa and green algae Chlorella luteoviridis by paraquat-induced oxidative stress was monitored. The results showed that paraquat-induced PCD in the two species belonged to the caspase-dependent pathway. Dose- and time-dependent PCD characteristics in the two strains under paraquat included the increase in caspase-like activity, DNA fragmentation, and chromatin condensation. However, the signaling pathway and cascade events of PCD in M. aeruginosa and C. luteoviridis differed. In M. aeruginosa, the free Ca2+ concentration was rapidly increased at 8 h, followed by a significant elevation of the reactive oxygen species (ROS) level at 24 h, and eventual cell death. In C. luteoviridis, the mitochondrial apoptosis pathway, revealed by the depolarization of the mitochondrial membrane potential at 1 h and increase in the ROS level and caspase-like activity at 8 h, might contribute to cell death. In addition, the dynamics of ROS levels and metacaspase activity were synchronized, suggesting that paraquat-triggered PCD was ROS-mediated in both M. aeruginosa and C. luteoviridis. These results provide insights into PCD patterns in prokaryotic cyanobacteria and eukaryotic green algae under similar stress. |
| first_indexed | 2024-04-11T00:56:40Z |
| format | Article |
| id | doaj.art-8a8981e119964575a0d474436ab434c6 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-11T00:56:40Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-8a8981e119964575a0d474436ab434c62023-01-05T04:30:39ZengElsevierEcotoxicology and Environmental Safety0147-65132023-01-01249114429Paraquat induces different programmed cell death patterns in Microcystis aeruginosa and Chlorella luteoviridisFang Bai0Yunlu Jia1Jie Li2Zhongxing Wu3Lin Li4Lirong Song5State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; Corresponding authors.School of Life Sciences, Central South University, Changsha 410013, ChinaKey Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing 400715, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; Corresponding authors.Although programmed cell death (PCD) has been reported in phytoplankton, knowledge of the characterization of the PCD pathway and cascade process in different phytoplankton species is still limited. In this study, PCD progression in cyanobacterium Microcystis aeruginosa and green algae Chlorella luteoviridis by paraquat-induced oxidative stress was monitored. The results showed that paraquat-induced PCD in the two species belonged to the caspase-dependent pathway. Dose- and time-dependent PCD characteristics in the two strains under paraquat included the increase in caspase-like activity, DNA fragmentation, and chromatin condensation. However, the signaling pathway and cascade events of PCD in M. aeruginosa and C. luteoviridis differed. In M. aeruginosa, the free Ca2+ concentration was rapidly increased at 8 h, followed by a significant elevation of the reactive oxygen species (ROS) level at 24 h, and eventual cell death. In C. luteoviridis, the mitochondrial apoptosis pathway, revealed by the depolarization of the mitochondrial membrane potential at 1 h and increase in the ROS level and caspase-like activity at 8 h, might contribute to cell death. In addition, the dynamics of ROS levels and metacaspase activity were synchronized, suggesting that paraquat-triggered PCD was ROS-mediated in both M. aeruginosa and C. luteoviridis. These results provide insights into PCD patterns in prokaryotic cyanobacteria and eukaryotic green algae under similar stress.http://www.sciencedirect.com/science/article/pii/S0147651322012696Programmed cell deathMicrocystis aeruginosaChlorella luteoviridisROSParaquat |
| spellingShingle | Fang Bai Yunlu Jia Jie Li Zhongxing Wu Lin Li Lirong Song Paraquat induces different programmed cell death patterns in Microcystis aeruginosa and Chlorella luteoviridis Ecotoxicology and Environmental Safety Programmed cell death Microcystis aeruginosa Chlorella luteoviridis ROS Paraquat |
| title | Paraquat induces different programmed cell death patterns in Microcystis aeruginosa and Chlorella luteoviridis |
| title_full | Paraquat induces different programmed cell death patterns in Microcystis aeruginosa and Chlorella luteoviridis |
| title_fullStr | Paraquat induces different programmed cell death patterns in Microcystis aeruginosa and Chlorella luteoviridis |
| title_full_unstemmed | Paraquat induces different programmed cell death patterns in Microcystis aeruginosa and Chlorella luteoviridis |
| title_short | Paraquat induces different programmed cell death patterns in Microcystis aeruginosa and Chlorella luteoviridis |
| title_sort | paraquat induces different programmed cell death patterns in microcystis aeruginosa and chlorella luteoviridis |
| topic | Programmed cell death Microcystis aeruginosa Chlorella luteoviridis ROS Paraquat |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322012696 |
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