Corrigendum to “Do human sinoatrial node cells have t-tubules?” [Transl. Res. Anat. 25C (2021) 100131]
Background and purpose: Every heartbeat is generated by spontaneously depolarizing specialised nodal cells that make up the sinoatrial node (SN). The SN's unique properties depend on two complex mechanisms - the membrane-voltage and the Ca2+ clocks, regulated by strictly defined expression of i...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Translational Research in Anatomy |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214854X22000218 |
| _version_ | 1818212159380783104 |
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| author | Maria A. Petkova Halina Dobrzynski |
| author_facet | Maria A. Petkova Halina Dobrzynski |
| author_sort | Maria A. Petkova |
| collection | DOAJ |
| description | Background and purpose: Every heartbeat is generated by spontaneously depolarizing specialised nodal cells that make up the sinoatrial node (SN). The SN's unique properties depend on two complex mechanisms - the membrane-voltage and the Ca2+ clocks, regulated by strictly defined expression of ion channels, connexins and Ca2+-handling proteins. In the working myocardium these proteins are accumulated in specialised membrane structures– the transverse tubules (t-tubules). It is well known that small mammals do not exhibit t-tubules in their atrium, but such structures are observed in the atria of bigger mammals such as sheep. No information on t-tubules in the human SN is present yet. The aim of our study was to determine if t-tubules are present in the human SN, as well as surrounding atrial myocardium. Basic procedures: Dystrophin (cytoplasmic protein connecting the muscle fibres to the surrounding extracellular membrane), Na+-Ca2+ exchanger (NCX1), sarcoplasmic-endoplasmic reticulum calcium adenosine triphosphatase (SERCA2a), α-actinin, and ryanodine receptor 2 (RyR2) were investigated. Main findings and principal conclusions: Staining with wheat germ agglutinin (WGA, specific marker of t-tubules) demonstrated that human RA cells contain t-tubules, but these structures are very sparse in the SN. Experiments in single nodal cells from rat and mouse showed no expression of t-tubular network, but striated pattern of expression of RyR2 was obtained similar to the human sections, suggesting its manner of organization is dependent on α-actinin colocalization. |
| first_indexed | 2024-12-12T05:43:58Z |
| format | Article |
| id | doaj.art-8a8b61f87ed04ce6ac041f74bc9dc313 |
| institution | Directory Open Access Journal |
| issn | 2214-854X |
| language | English |
| last_indexed | 2024-12-12T05:43:58Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Translational Research in Anatomy |
| spelling | doaj.art-8a8b61f87ed04ce6ac041f74bc9dc3132022-12-22T00:35:50ZengElsevierTranslational Research in Anatomy2214-854X2022-06-0127100177Corrigendum to “Do human sinoatrial node cells have t-tubules?” [Transl. Res. Anat. 25C (2021) 100131]Maria A. Petkova0Halina Dobrzynski1Division of Cardiovascular Sciences, Faculty of Biology, Medicine and Health Manchester, University of Manchester, CTF Building, 46 Grafton Street, M13 9NT, Manchester, UKDivision of Cardiovascular Sciences, Faculty of Biology, Medicine and Health Manchester, University of Manchester, CTF Building, 46 Grafton Street, M13 9NT, Manchester, UK; Department of Anatomy, Jagiellonian University Medical College, Świętej Anny 12, 31-008, Krakow, Poland; Corresponding author. Division of Cardiovascular Sciences, Faculty of Biology, Medicine and Health Manchester, University of Manchester, CTF Building, 46 Grafton Street, M13 9NT, Manchester, UK.Background and purpose: Every heartbeat is generated by spontaneously depolarizing specialised nodal cells that make up the sinoatrial node (SN). The SN's unique properties depend on two complex mechanisms - the membrane-voltage and the Ca2+ clocks, regulated by strictly defined expression of ion channels, connexins and Ca2+-handling proteins. In the working myocardium these proteins are accumulated in specialised membrane structures– the transverse tubules (t-tubules). It is well known that small mammals do not exhibit t-tubules in their atrium, but such structures are observed in the atria of bigger mammals such as sheep. No information on t-tubules in the human SN is present yet. The aim of our study was to determine if t-tubules are present in the human SN, as well as surrounding atrial myocardium. Basic procedures: Dystrophin (cytoplasmic protein connecting the muscle fibres to the surrounding extracellular membrane), Na+-Ca2+ exchanger (NCX1), sarcoplasmic-endoplasmic reticulum calcium adenosine triphosphatase (SERCA2a), α-actinin, and ryanodine receptor 2 (RyR2) were investigated. Main findings and principal conclusions: Staining with wheat germ agglutinin (WGA, specific marker of t-tubules) demonstrated that human RA cells contain t-tubules, but these structures are very sparse in the SN. Experiments in single nodal cells from rat and mouse showed no expression of t-tubular network, but striated pattern of expression of RyR2 was obtained similar to the human sections, suggesting its manner of organization is dependent on α-actinin colocalization.http://www.sciencedirect.com/science/article/pii/S2214854X22000218 |
| spellingShingle | Maria A. Petkova Halina Dobrzynski Corrigendum to “Do human sinoatrial node cells have t-tubules?” [Transl. Res. Anat. 25C (2021) 100131] Translational Research in Anatomy |
| title | Corrigendum to “Do human sinoatrial node cells have t-tubules?” [Transl. Res. Anat. 25C (2021) 100131] |
| title_full | Corrigendum to “Do human sinoatrial node cells have t-tubules?” [Transl. Res. Anat. 25C (2021) 100131] |
| title_fullStr | Corrigendum to “Do human sinoatrial node cells have t-tubules?” [Transl. Res. Anat. 25C (2021) 100131] |
| title_full_unstemmed | Corrigendum to “Do human sinoatrial node cells have t-tubules?” [Transl. Res. Anat. 25C (2021) 100131] |
| title_short | Corrigendum to “Do human sinoatrial node cells have t-tubules?” [Transl. Res. Anat. 25C (2021) 100131] |
| title_sort | corrigendum to do human sinoatrial node cells have t tubules transl res anat 25c 2021 100131 |
| url | http://www.sciencedirect.com/science/article/pii/S2214854X22000218 |
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