Wide Distribution of <i>Teratosphaeria epicoccoides</i> and <i>T. destructans</i> Associated with Diseased <i>Eucalyptus</i> Leaves in Plantations in Southern China
Species of <i>Mycosphaerellaceae</i> and <i>Teratosphaeriaceae</i> represent over 40% of the fungi identified on eucalypt leaves worldwide. These include some important pathogens that mainly cause leaf blight and spot, and result in increasingly negative impacts on global com...
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MDPI AG
2024-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/12/1/129 |
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| author | Bingyin Chen Wenxia Wu Shuaifei Chen |
| author_facet | Bingyin Chen Wenxia Wu Shuaifei Chen |
| author_sort | Bingyin Chen |
| collection | DOAJ |
| description | Species of <i>Mycosphaerellaceae</i> and <i>Teratosphaeriaceae</i> represent over 40% of the fungi identified on eucalypt leaves worldwide. These include some important pathogens that mainly cause leaf blight and spot, and result in increasingly negative impacts on global commercial eucalypt industries. <i>Eucalyptus</i> plantations are commonly cultivated in southern China for solid wood and pulp products. However, the species diversity and geographic distribution of <i>Mycosphaerellaceae</i> and <i>Teratosphaeriaceae</i>, associated with diseased plantation <i>Eucalyptus</i> leaves in China, have not been clarified. In this study, we conducted the first systematic surveys and sample collections of <i>Mycosphaerellaceae</i>- and <i>Teratosphaeriaceae</i>-like fungi from diseased plantation <i>Eucalyptus</i> leaves in southern China. In total, 558 isolates were obtained from 59 sampled sites in five provinces. One isolate was isolated from each tree. According to the disease symptoms, conidia morphological characteristics, and DNA sequence comparisons of ITS, <i>tef1</i> and <i>tub2</i> gene regions. The 558 isolates were identified as <i>Teratosphaeria epicoccoides</i> (312 isolates; 55.9%) and <i>T. destructans</i> (246 isolates, 44.1%). Both species were widely distributed in the sampled regions in southern China. The genotypes of <i>T. epicoccoides</i> and <i>T. destructans</i> were determined based on ITS, <i>tef1</i>, and <i>tub2</i> sequences. The results showed that multiple genotypes of each species of <i>T. epicoccoides</i> and <i>T. destructans</i> exist in China. Additionally, isolates with multiple genotypes were obtained in all five sampled provinces. These results suggest that both <i>T. epicoccoides</i> and <i>T. destructans</i> are not clonal. This study proved that both <i>T. epicoccoides</i> and <i>T. destructans</i> are dominant species and widely distributed on diseased <i>Eucalyptus</i> leaves in southern China. The wide geographic distribution and potential high genetic diversity pose challenges for the disease management of <i>Teratosphaeria</i> leaf blight and leaf spot in China. |
| first_indexed | 2024-03-08T09:49:49Z |
| format | Article |
| id | doaj.art-8a8c71e31a374dd48da0d8d83d290913 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-08T09:49:49Z |
| publishDate | 2024-01-01 |
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| spelling | doaj.art-8a8c71e31a374dd48da0d8d83d2909132024-01-29T14:06:28ZengMDPI AGMicroorganisms2076-26072024-01-0112112910.3390/microorganisms12010129Wide Distribution of <i>Teratosphaeria epicoccoides</i> and <i>T. destructans</i> Associated with Diseased <i>Eucalyptus</i> Leaves in Plantations in Southern ChinaBingyin Chen0Wenxia Wu1Shuaifei Chen2Research Institute of Fast-Growing Trees (RIFT), Chinese Academy of Forestry (CAF), Zhanjiang 524022, ChinaResearch Institute of Fast-Growing Trees (RIFT), Chinese Academy of Forestry (CAF), Zhanjiang 524022, ChinaResearch Institute of Fast-Growing Trees (RIFT), Chinese Academy of Forestry (CAF), Zhanjiang 524022, ChinaSpecies of <i>Mycosphaerellaceae</i> and <i>Teratosphaeriaceae</i> represent over 40% of the fungi identified on eucalypt leaves worldwide. These include some important pathogens that mainly cause leaf blight and spot, and result in increasingly negative impacts on global commercial eucalypt industries. <i>Eucalyptus</i> plantations are commonly cultivated in southern China for solid wood and pulp products. However, the species diversity and geographic distribution of <i>Mycosphaerellaceae</i> and <i>Teratosphaeriaceae</i>, associated with diseased plantation <i>Eucalyptus</i> leaves in China, have not been clarified. In this study, we conducted the first systematic surveys and sample collections of <i>Mycosphaerellaceae</i>- and <i>Teratosphaeriaceae</i>-like fungi from diseased plantation <i>Eucalyptus</i> leaves in southern China. In total, 558 isolates were obtained from 59 sampled sites in five provinces. One isolate was isolated from each tree. According to the disease symptoms, conidia morphological characteristics, and DNA sequence comparisons of ITS, <i>tef1</i> and <i>tub2</i> gene regions. The 558 isolates were identified as <i>Teratosphaeria epicoccoides</i> (312 isolates; 55.9%) and <i>T. destructans</i> (246 isolates, 44.1%). Both species were widely distributed in the sampled regions in southern China. The genotypes of <i>T. epicoccoides</i> and <i>T. destructans</i> were determined based on ITS, <i>tef1</i>, and <i>tub2</i> sequences. The results showed that multiple genotypes of each species of <i>T. epicoccoides</i> and <i>T. destructans</i> exist in China. Additionally, isolates with multiple genotypes were obtained in all five sampled provinces. These results suggest that both <i>T. epicoccoides</i> and <i>T. destructans</i> are not clonal. This study proved that both <i>T. epicoccoides</i> and <i>T. destructans</i> are dominant species and widely distributed on diseased <i>Eucalyptus</i> leaves in southern China. The wide geographic distribution and potential high genetic diversity pose challenges for the disease management of <i>Teratosphaeria</i> leaf blight and leaf spot in China.https://www.mdpi.com/2076-2607/12/1/129forest diseasegenetic diversityleaf pathogenphylogeny<i>Teratosphaeria</i> leaf blight<i>Teratosphaeria</i> leaf spot |
| spellingShingle | Bingyin Chen Wenxia Wu Shuaifei Chen Wide Distribution of <i>Teratosphaeria epicoccoides</i> and <i>T. destructans</i> Associated with Diseased <i>Eucalyptus</i> Leaves in Plantations in Southern China Microorganisms forest disease genetic diversity leaf pathogen phylogeny <i>Teratosphaeria</i> leaf blight <i>Teratosphaeria</i> leaf spot |
| title | Wide Distribution of <i>Teratosphaeria epicoccoides</i> and <i>T. destructans</i> Associated with Diseased <i>Eucalyptus</i> Leaves in Plantations in Southern China |
| title_full | Wide Distribution of <i>Teratosphaeria epicoccoides</i> and <i>T. destructans</i> Associated with Diseased <i>Eucalyptus</i> Leaves in Plantations in Southern China |
| title_fullStr | Wide Distribution of <i>Teratosphaeria epicoccoides</i> and <i>T. destructans</i> Associated with Diseased <i>Eucalyptus</i> Leaves in Plantations in Southern China |
| title_full_unstemmed | Wide Distribution of <i>Teratosphaeria epicoccoides</i> and <i>T. destructans</i> Associated with Diseased <i>Eucalyptus</i> Leaves in Plantations in Southern China |
| title_short | Wide Distribution of <i>Teratosphaeria epicoccoides</i> and <i>T. destructans</i> Associated with Diseased <i>Eucalyptus</i> Leaves in Plantations in Southern China |
| title_sort | wide distribution of i teratosphaeria epicoccoides i and i t destructans i associated with diseased i eucalyptus i leaves in plantations in southern china |
| topic | forest disease genetic diversity leaf pathogen phylogeny <i>Teratosphaeria</i> leaf blight <i>Teratosphaeria</i> leaf spot |
| url | https://www.mdpi.com/2076-2607/12/1/129 |
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