Serotype distribution and antimicrobial Susceptibility of Streptococcus pneumoniae isolated from patients with pneumococcal pneumonia
Introduction: Streptococcus pneumoniae is a major cause of community-acquired pneumonia (CAP). In Serbia, pneumococcal conjugate vaccines PCV-10 and PCV-13 were licensed for immunization in 2018. Aim: The aim of this study was to evaluate serotype distribution and antimicrobial susceptibility of S....
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| Format: | Article |
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University of Belgrade, Medical Faculty
2021-01-01
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| Series: | Medicinski Podmladak |
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| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/0369-1527/2021/0369-15272104008H.pdf |
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| author | Hamzagić Farah Ranin Jovana Gajić Ina Opavski Nataša Ranin Lazar |
| author_facet | Hamzagić Farah Ranin Jovana Gajić Ina Opavski Nataša Ranin Lazar |
| author_sort | Hamzagić Farah |
| collection | DOAJ |
| description | Introduction: Streptococcus pneumoniae is a major cause of community-acquired pneumonia (CAP). In Serbia, pneumococcal conjugate vaccines PCV-10 and PCV-13 were licensed for immunization in 2018. Aim: The aim of this study was to evaluate serotype distribution and antimicrobial susceptibility of S. pneumoniae isolated from patients with pneumococcal pneumonia. Material and methods: Isolates of S. pneumoniae were recovered from patients with CAP addmitted to 18 hospitals throughout Serbia from 2012-2020. Confirmation of the identification was performed using lytA gene detection by PCR. Serotyping and antimicrobial susceptibility testing was performed by Quellung reaction and disk diffusion/gradient test, respectively. Interpretation of the susceptibility testing was done according to the current European Committee on Antimicrobial Susceptibility Testing recommendations. Results: During the study period, a total of 100 isolates of S. pneumoniae were collected. Overall, 19 pneumococcal serotypes were identified. The predominant types observed, in order of decreasing frequency, were 3 (37%), 14 (16%), 6B (6%), 7F (5%), 9V (4%), and 4 (4%); further, 2% of the isolates were non-typeable. However, the most common serotype among children < 2 years old was 14 (27.3%). The vaccine coverage of strains isolated from children < 5 years was higher with PCV-13 compared to PCV-10 (86,7% vs. 46,7%, p = 0.008). Additionally, the vaccine coverage of pneumococci isolated from patients >2 years was higher with the PPV-23 than with PCV-13 (96.6% vs. 84.3%, p = 0.0230). Ten isolates (10%) were PPV-23/nonPCV-13, whereas four isolates (4%) were non-typable (17A, 23A, and 9A). Serotype 3 significantly increased from pre-vaccine to post-vaccine period (30.8% vs. 60.8%, p = 0.0108). The overall antimicrobial resistance rates were as follows: penicillin - 9%, ceftriaxone - 2%, erythromycin - 24%, clindamycin - 16%, tetracycline - 11%, and trimethoprim-sulfamethoxazole - 21%. Conclusion: More than 70% of the isolated strains belonged to the serotypes 3, 14, 6B, 9B, and 4. The highest resistance rates of the tested pneumococci were found for macrolides and trimethoprim-sulfamethoxazole. |
| first_indexed | 2024-12-18T11:08:53Z |
| format | Article |
| id | doaj.art-8a8fe81f98cb4284a60c72ff9ca38a0d |
| institution | Directory Open Access Journal |
| issn | 0369-1527 2466-5525 |
| language | English |
| last_indexed | 2024-12-18T11:08:53Z |
| publishDate | 2021-01-01 |
| publisher | University of Belgrade, Medical Faculty |
| record_format | Article |
| series | Medicinski Podmladak |
| spelling | doaj.art-8a8fe81f98cb4284a60c72ff9ca38a0d2022-12-21T21:10:01ZengUniversity of Belgrade, Medical FacultyMedicinski Podmladak0369-15272466-55252021-01-0172481310.5937/mp72-334960369-15272104008HSerotype distribution and antimicrobial Susceptibility of Streptococcus pneumoniae isolated from patients with pneumococcal pneumoniaHamzagić Farah0Ranin Jovana1Gajić Ina2https://orcid.org/0000-0002-1578-2776Opavski Nataša3https://orcid.org/0000-0002-8765-4044Ranin Lazar4Univerzitet u Beogradu, Medicinski fakultet, SerbiaUniverzitet u Beogradu, Medicinski fakultet, SerbiaUniverzitet u Beogradu, Medicinski fakultet, SerbiaUniverzitet u Beogradu, Medicinski fakultet, SerbiaUniverzitet u Beogradu, Medicinski fakultet, SerbiaIntroduction: Streptococcus pneumoniae is a major cause of community-acquired pneumonia (CAP). In Serbia, pneumococcal conjugate vaccines PCV-10 and PCV-13 were licensed for immunization in 2018. Aim: The aim of this study was to evaluate serotype distribution and antimicrobial susceptibility of S. pneumoniae isolated from patients with pneumococcal pneumonia. Material and methods: Isolates of S. pneumoniae were recovered from patients with CAP addmitted to 18 hospitals throughout Serbia from 2012-2020. Confirmation of the identification was performed using lytA gene detection by PCR. Serotyping and antimicrobial susceptibility testing was performed by Quellung reaction and disk diffusion/gradient test, respectively. Interpretation of the susceptibility testing was done according to the current European Committee on Antimicrobial Susceptibility Testing recommendations. Results: During the study period, a total of 100 isolates of S. pneumoniae were collected. Overall, 19 pneumococcal serotypes were identified. The predominant types observed, in order of decreasing frequency, were 3 (37%), 14 (16%), 6B (6%), 7F (5%), 9V (4%), and 4 (4%); further, 2% of the isolates were non-typeable. However, the most common serotype among children < 2 years old was 14 (27.3%). The vaccine coverage of strains isolated from children < 5 years was higher with PCV-13 compared to PCV-10 (86,7% vs. 46,7%, p = 0.008). Additionally, the vaccine coverage of pneumococci isolated from patients >2 years was higher with the PPV-23 than with PCV-13 (96.6% vs. 84.3%, p = 0.0230). Ten isolates (10%) were PPV-23/nonPCV-13, whereas four isolates (4%) were non-typable (17A, 23A, and 9A). Serotype 3 significantly increased from pre-vaccine to post-vaccine period (30.8% vs. 60.8%, p = 0.0108). The overall antimicrobial resistance rates were as follows: penicillin - 9%, ceftriaxone - 2%, erythromycin - 24%, clindamycin - 16%, tetracycline - 11%, and trimethoprim-sulfamethoxazole - 21%. Conclusion: More than 70% of the isolated strains belonged to the serotypes 3, 14, 6B, 9B, and 4. The highest resistance rates of the tested pneumococci were found for macrolides and trimethoprim-sulfamethoxazole.https://scindeks-clanci.ceon.rs/data/pdf/0369-1527/2021/0369-15272104008H.pdfstreptococcus pneumoniaeserotypesantimicrobial resistancepneumonia |
| spellingShingle | Hamzagić Farah Ranin Jovana Gajić Ina Opavski Nataša Ranin Lazar Serotype distribution and antimicrobial Susceptibility of Streptococcus pneumoniae isolated from patients with pneumococcal pneumonia Medicinski Podmladak streptococcus pneumoniae serotypes antimicrobial resistance pneumonia |
| title | Serotype distribution and antimicrobial Susceptibility of Streptococcus pneumoniae isolated from patients with pneumococcal pneumonia |
| title_full | Serotype distribution and antimicrobial Susceptibility of Streptococcus pneumoniae isolated from patients with pneumococcal pneumonia |
| title_fullStr | Serotype distribution and antimicrobial Susceptibility of Streptococcus pneumoniae isolated from patients with pneumococcal pneumonia |
| title_full_unstemmed | Serotype distribution and antimicrobial Susceptibility of Streptococcus pneumoniae isolated from patients with pneumococcal pneumonia |
| title_short | Serotype distribution and antimicrobial Susceptibility of Streptococcus pneumoniae isolated from patients with pneumococcal pneumonia |
| title_sort | serotype distribution and antimicrobial susceptibility of streptococcus pneumoniae isolated from patients with pneumococcal pneumonia |
| topic | streptococcus pneumoniae serotypes antimicrobial resistance pneumonia |
| url | https://scindeks-clanci.ceon.rs/data/pdf/0369-1527/2021/0369-15272104008H.pdf |
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