Betaine affects abdominal flare fat metabolism via regulating m6A RNA methylation in finishing pigs fed a low-energy diet
This study was conducted to investigate the effects of betaine on lipid metabolism and m6A RNA methylation in finishing pigs fed a low-energy diet. The results showed that betaine significantly enhanced serum concentration of triglyceride and reduced the relative weight of flare fat (P < 0.05) in...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-08-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623002207 |
| _version_ | 1797749183265898496 |
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| author | Runqi Fu Daiwen Chen Gang Tian Ping Zheng Jun He Jie Yu Xiangbing Mao Zhiqing Huang Junning Pu Bing Yu |
| author_facet | Runqi Fu Daiwen Chen Gang Tian Ping Zheng Jun He Jie Yu Xiangbing Mao Zhiqing Huang Junning Pu Bing Yu |
| author_sort | Runqi Fu |
| collection | DOAJ |
| description | This study was conducted to investigate the effects of betaine on lipid metabolism and m6A RNA methylation in finishing pigs fed a low-energy diet. The results showed that betaine significantly enhanced serum concentration of triglyceride and reduced the relative weight of flare fat (P < 0.05) in pigs fed a low-energy diet, as well as, changed mRNA or protein expression of genes related to lipogenesis and lipolysis (P < 0.05). Betaine reversed the reduction in mRNA or protein abundances of YTH N6-methyladenosine RNA-binding protein 1 (YTHDF1) and YTHDF2 caused by low-energy diet, and increased the levels of m6A RNA methylation and the expressions of methyltransferase-like 3 (METTL3), METTL14 (P < 0.05). Collectively, betaine decreased the flare fat content of finishing pigs exposed to low-energy diet by reducing lipogenesis and increasing lipolysis, and this phenotypic change was attributed to the elevation of m6A RNA methylation level by betaine. |
| first_indexed | 2024-03-12T16:15:36Z |
| format | Article |
| id | doaj.art-8a915fd8d128478fa24ce193bf29773f |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-03-12T16:15:36Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-8a915fd8d128478fa24ce193bf29773f2023-08-09T04:32:20ZengElsevierJournal of Functional Foods1756-46462023-08-01107105620Betaine affects abdominal flare fat metabolism via regulating m6A RNA methylation in finishing pigs fed a low-energy dietRunqi Fu0Daiwen Chen1Gang Tian2Ping Zheng3Jun He4Jie Yu5Xiangbing Mao6Zhiqing Huang7Junning Pu8Bing Yu9Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan 611130, China; Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, ChinaKey Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan 611130, China; Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, ChinaKey Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan 611130, China; Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, ChinaKey Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan 611130, China; Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, ChinaKey Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan 611130, China; Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, ChinaKey Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan 611130, China; Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, ChinaKey Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan 611130, China; Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, ChinaKey Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan 611130, China; Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, ChinaKey Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan 611130, China; Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, ChinaCorresponding author.; Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan 611130, China; Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, ChinaThis study was conducted to investigate the effects of betaine on lipid metabolism and m6A RNA methylation in finishing pigs fed a low-energy diet. The results showed that betaine significantly enhanced serum concentration of triglyceride and reduced the relative weight of flare fat (P < 0.05) in pigs fed a low-energy diet, as well as, changed mRNA or protein expression of genes related to lipogenesis and lipolysis (P < 0.05). Betaine reversed the reduction in mRNA or protein abundances of YTH N6-methyladenosine RNA-binding protein 1 (YTHDF1) and YTHDF2 caused by low-energy diet, and increased the levels of m6A RNA methylation and the expressions of methyltransferase-like 3 (METTL3), METTL14 (P < 0.05). Collectively, betaine decreased the flare fat content of finishing pigs exposed to low-energy diet by reducing lipogenesis and increasing lipolysis, and this phenotypic change was attributed to the elevation of m6A RNA methylation level by betaine.http://www.sciencedirect.com/science/article/pii/S1756464623002207BetaineEpigeneticFlare fatFinishing pigsLipid metabolismm6A RNA methylation |
| spellingShingle | Runqi Fu Daiwen Chen Gang Tian Ping Zheng Jun He Jie Yu Xiangbing Mao Zhiqing Huang Junning Pu Bing Yu Betaine affects abdominal flare fat metabolism via regulating m6A RNA methylation in finishing pigs fed a low-energy diet Journal of Functional Foods Betaine Epigenetic Flare fat Finishing pigs Lipid metabolism m6A RNA methylation |
| title | Betaine affects abdominal flare fat metabolism via regulating m6A RNA methylation in finishing pigs fed a low-energy diet |
| title_full | Betaine affects abdominal flare fat metabolism via regulating m6A RNA methylation in finishing pigs fed a low-energy diet |
| title_fullStr | Betaine affects abdominal flare fat metabolism via regulating m6A RNA methylation in finishing pigs fed a low-energy diet |
| title_full_unstemmed | Betaine affects abdominal flare fat metabolism via regulating m6A RNA methylation in finishing pigs fed a low-energy diet |
| title_short | Betaine affects abdominal flare fat metabolism via regulating m6A RNA methylation in finishing pigs fed a low-energy diet |
| title_sort | betaine affects abdominal flare fat metabolism via regulating m6a rna methylation in finishing pigs fed a low energy diet |
| topic | Betaine Epigenetic Flare fat Finishing pigs Lipid metabolism m6A RNA methylation |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623002207 |
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