Antimicrobial Activity and LC Determination of Ethanol Crude Extract of 50 Pliek U, an Achehnese Traditional Fermented Food

Antimicrobial activity of pliek u crude extracts was evaluated against five bacterial species (Staphylococcus aureus, Bacillus cereus, Escherichia coli, Salmonella enterica serovar Enteritidis and Pseudomonas aeruginosa,) and one fungal species (Candida albicans). Pliek u has been consumed as spices...

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Main Authors: Nurliana Nurliana, Mirnawati Sudarwanto, Lisdar I. Sudirman, Angelina W. Sanjaya
Format: Article
Language:Indonesian
Published: Syiah Kuala University 2010-02-01
Series:Jurnal Kedokteran Hewan
Subjects:
Online Access:https://jurnal.usk.ac.id/JKH/article/view/9795
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author Nurliana Nurliana
Mirnawati Sudarwanto
Lisdar I. Sudirman
Angelina W. Sanjaya
author_facet Nurliana Nurliana
Mirnawati Sudarwanto
Lisdar I. Sudirman
Angelina W. Sanjaya
author_sort Nurliana Nurliana
collection DOAJ
description Antimicrobial activity of pliek u crude extracts was evaluated against five bacterial species (Staphylococcus aureus, Bacillus cereus, Escherichia coli, Salmonella enterica serovar Enteritidis and Pseudomonas aeruginosa,) and one fungal species (Candida albicans). Pliek u has been consumed as spices and hot sauce and poultry feed. These foods were collected from household industry at Reudep village in Aceh Besar, Province of Aceh. The ethanol extract of pliek u were obtained by standard method. The antimicrobial activity was detected using paper disc method. The concentration of ethanol crude extract of pliek u (EEP) was determined with the dilution method. The lethality initial test has been detected by using Artemia salina L. bioassay to determine the toxic concentration based on the LC value of EEP. The ethanol crude extract (EEP) was active against all microbial strains. EEP showed 50 antimicrobial activity at a minimum inhibitory concentration (MIC) and a minimum microbicidal concentration (MMC) at 2.5-10 mg/ml and 10-20 mg/ml, respectively. The lethality concentration EEP gave the LC value of 3.36 50 mg/ml. It was concluded that ethanol crude extract (EEP) shows significant antimicrobial activity and it is not toxic.
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spelling doaj.art-8a92bc6c2a16464fb25a009bb0e246cd2024-03-04T10:28:57ZindSyiah Kuala UniversityJurnal Kedokteran Hewan1978-225X2502-56002010-02-014110.21157/j.ked.hewan.v4i1.97957569Antimicrobial Activity and LC Determination of Ethanol Crude Extract of 50 Pliek U, an Achehnese Traditional Fermented FoodNurliana Nurliana0Mirnawati Sudarwanto1Lisdar I. Sudirman2Angelina W. Sanjaya3Fakultas Kedokteran Hewan, Universitas Syiah Kuala, Banda AcehFakultas Kedokteran Hewan, Institut Pertanian Bogor, BogorFakultas MIPA, Institut Pertanian Bogor, BogorFakultas Kedokteran Hewan, Institut Pertanian Bogor, BogorAntimicrobial activity of pliek u crude extracts was evaluated against five bacterial species (Staphylococcus aureus, Bacillus cereus, Escherichia coli, Salmonella enterica serovar Enteritidis and Pseudomonas aeruginosa,) and one fungal species (Candida albicans). Pliek u has been consumed as spices and hot sauce and poultry feed. These foods were collected from household industry at Reudep village in Aceh Besar, Province of Aceh. The ethanol extract of pliek u were obtained by standard method. The antimicrobial activity was detected using paper disc method. The concentration of ethanol crude extract of pliek u (EEP) was determined with the dilution method. The lethality initial test has been detected by using Artemia salina L. bioassay to determine the toxic concentration based on the LC value of EEP. The ethanol crude extract (EEP) was active against all microbial strains. EEP showed 50 antimicrobial activity at a minimum inhibitory concentration (MIC) and a minimum microbicidal concentration (MMC) at 2.5-10 mg/ml and 10-20 mg/ml, respectively. The lethality concentration EEP gave the LC value of 3.36 50 mg/ml. It was concluded that ethanol crude extract (EEP) shows significant antimicrobial activity and it is not toxic.https://jurnal.usk.ac.id/JKH/article/view/9795pliek uantimicrobial activityartemia salina l. bioassay
spellingShingle Nurliana Nurliana
Mirnawati Sudarwanto
Lisdar I. Sudirman
Angelina W. Sanjaya
Antimicrobial Activity and LC Determination of Ethanol Crude Extract of 50 Pliek U, an Achehnese Traditional Fermented Food
Jurnal Kedokteran Hewan
pliek u
antimicrobial activity
artemia salina l. bioassay
title Antimicrobial Activity and LC Determination of Ethanol Crude Extract of 50 Pliek U, an Achehnese Traditional Fermented Food
title_full Antimicrobial Activity and LC Determination of Ethanol Crude Extract of 50 Pliek U, an Achehnese Traditional Fermented Food
title_fullStr Antimicrobial Activity and LC Determination of Ethanol Crude Extract of 50 Pliek U, an Achehnese Traditional Fermented Food
title_full_unstemmed Antimicrobial Activity and LC Determination of Ethanol Crude Extract of 50 Pliek U, an Achehnese Traditional Fermented Food
title_short Antimicrobial Activity and LC Determination of Ethanol Crude Extract of 50 Pliek U, an Achehnese Traditional Fermented Food
title_sort antimicrobial activity and lc determination of ethanol crude extract of 50 pliek u an achehnese traditional fermented food
topic pliek u
antimicrobial activity
artemia salina l. bioassay
url https://jurnal.usk.ac.id/JKH/article/view/9795
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