Aeromonas veronii Causes Hemorrhagic Septicemia in Cultured Nile tilapia in Qena Governorate
n summer 2021, acute mortalities occurred in Nile tilapia farm in Qena governorate. Different hypothesis including the implication of environmental factors, and/or pathogens have been explored. Clinical, parasitological and bacteriological examinations have been performed. In addition, the water qua...
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| Format: | Article |
| Language: | English |
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South Valley University
2022-12-01
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| Series: | SVU-International Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | https://svu.journals.ekb.eg/article_275750.html |
| _version_ | 1797799370889887744 |
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| author | Karima A. Bakry Walaa F.A. Emeish |
| author_facet | Karima A. Bakry Walaa F.A. Emeish |
| author_sort | Karima A. Bakry |
| collection | DOAJ |
| description | n summer 2021, acute mortalities occurred in Nile tilapia farm in Qena governorate. Different hypothesis including the implication of environmental factors, and/or pathogens have been explored. Clinical, parasitological and bacteriological examinations have been performed. In addition, the water quality parameters were assessed. The clinical investigation of the moribund and recently dead fish demonstrated generalized septicaemia. The phenotypic and biochemical characterization of the pure bacterial colonies that isolated from 60 moribund fish revealed Gram-negative rods and identified as Aeromonas species. The identity of the isolates was identified as Aeromonas species by amplification of Aeromonas 16S rDNA gene fragment by PCR and confirmed as Aeromonas veronii by amplification and sequencing the Aeromonas gyrB-gene. The water quality parameters showed an increase in the pH and ammonia levels over the permissible levels. Experimental infections were performed to confirm the virulence of the isolated bacteria. Results revealed that A. veronii isolate produced the same clinical picture of the collected samples with mortality rate 80 %. The present study implicates that A. veronii could be the causative agent of the Nile tilapia mortality in Qena, without neglecting the role of water quality in worsening this outbreak. |
| first_indexed | 2024-03-13T04:17:51Z |
| format | Article |
| id | doaj.art-8a952458984b4be7aaad3c4fbe5fc0c4 |
| institution | Directory Open Access Journal |
| issn | 2535-1826 2535-1877 |
| language | English |
| last_indexed | 2024-03-13T04:17:51Z |
| publishDate | 2022-12-01 |
| publisher | South Valley University |
| record_format | Article |
| series | SVU-International Journal of Veterinary Sciences |
| spelling | doaj.art-8a952458984b4be7aaad3c4fbe5fc0c42023-06-20T18:19:19ZengSouth Valley UniversitySVU-International Journal of Veterinary Sciences2535-18262535-18772022-12-015412513710.21608/SVU.2022.155503.1221Aeromonas veronii Causes Hemorrhagic Septicemia in Cultured Nile tilapia in Qena GovernorateKarima A. Bakry0Walaa F.A. Emeish 1Department of Fish Diseases, Faculty of Veterinary Medicine, South Valley University, Qena 83523, EgyptDepartment of Fish Diseases, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egyptn summer 2021, acute mortalities occurred in Nile tilapia farm in Qena governorate. Different hypothesis including the implication of environmental factors, and/or pathogens have been explored. Clinical, parasitological and bacteriological examinations have been performed. In addition, the water quality parameters were assessed. The clinical investigation of the moribund and recently dead fish demonstrated generalized septicaemia. The phenotypic and biochemical characterization of the pure bacterial colonies that isolated from 60 moribund fish revealed Gram-negative rods and identified as Aeromonas species. The identity of the isolates was identified as Aeromonas species by amplification of Aeromonas 16S rDNA gene fragment by PCR and confirmed as Aeromonas veronii by amplification and sequencing the Aeromonas gyrB-gene. The water quality parameters showed an increase in the pH and ammonia levels over the permissible levels. Experimental infections were performed to confirm the virulence of the isolated bacteria. Results revealed that A. veronii isolate produced the same clinical picture of the collected samples with mortality rate 80 %. The present study implicates that A. veronii could be the causative agent of the Nile tilapia mortality in Qena, without neglecting the role of water quality in worsening this outbreak. https://svu.journals.ekb.eg/article_275750.htmlnile tilapiafish mortalitiesgene sequencinga. veronii |
| spellingShingle | Karima A. Bakry Walaa F.A. Emeish Aeromonas veronii Causes Hemorrhagic Septicemia in Cultured Nile tilapia in Qena Governorate SVU-International Journal of Veterinary Sciences nile tilapia fish mortalities gene sequencing a. veronii |
| title | Aeromonas veronii Causes Hemorrhagic Septicemia in Cultured Nile tilapia in Qena Governorate |
| title_full | Aeromonas veronii Causes Hemorrhagic Septicemia in Cultured Nile tilapia in Qena Governorate |
| title_fullStr | Aeromonas veronii Causes Hemorrhagic Septicemia in Cultured Nile tilapia in Qena Governorate |
| title_full_unstemmed | Aeromonas veronii Causes Hemorrhagic Septicemia in Cultured Nile tilapia in Qena Governorate |
| title_short | Aeromonas veronii Causes Hemorrhagic Septicemia in Cultured Nile tilapia in Qena Governorate |
| title_sort | aeromonas veronii causes hemorrhagic septicemia in cultured nile tilapia in qena governorate |
| topic | nile tilapia fish mortalities gene sequencing a. veronii |
| url | https://svu.journals.ekb.eg/article_275750.html |
| work_keys_str_mv | AT karimaabakry aeromonasveroniicauseshemorrhagicsepticemiainculturedniletilapiainqenagovernorate AT walaafaemeish aeromonasveroniicauseshemorrhagicsepticemiainculturedniletilapiainqenagovernorate |