Multispecies Identification of Oilseed- and Meat-Specific Proteins and Heat-Stable Peptide Markers in Food Products
Consumer demand for both plant products and meat products enriched with plant raw materials is constantly increasing. Therefore, new versatile and reliable methods are needed to find and combat fraudulent practices in processed foods. The objective of this study was to identify oilseed species-speci...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/6/1577 |
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| author | Klaudia Kotecka-Majchrzak Natalia Kasałka-Czarna Agata Sumara Emilia Fornal Magdalena Montowska |
| author_facet | Klaudia Kotecka-Majchrzak Natalia Kasałka-Czarna Agata Sumara Emilia Fornal Magdalena Montowska |
| author_sort | Klaudia Kotecka-Majchrzak |
| collection | DOAJ |
| description | Consumer demand for both plant products and meat products enriched with plant raw materials is constantly increasing. Therefore, new versatile and reliable methods are needed to find and combat fraudulent practices in processed foods. The objective of this study was to identify oilseed species-specific peptide markers and meat-specific markers that were resistant to processing, for multispecies authentication of different meat and vegan food products using the proteomic LC-MS/MS method. To assess the limit of detection (LOD) for hemp proteins, cooked meatballs consisting of three meat species and hemp cake at a final concentration of up to 7.4% were examined. Hemp addition at a low concentration of below 1% was detected. The LOD for edestin subunits and albumin was 0.9% (<i>w</i>/<i>w</i>), whereas for 7S vicilin-like protein it was 4.2% (<i>w</i>/<i>w</i>). Specific heat-stable peptides unique to hemp seeds, flaxseed, nigella, pumpkin, sesame, and sunflower seeds, as well as guinea fowl, rabbit, pork, and chicken meat, were detected in different meat and vegan foods. Most of the oilseed-specific peptides were identified as processing-resistant markers belonging to 11S globulin subunits, namely conlinin, edestin, helianthinin, pumpkin vicilin-like or late embryogenesis proteins, and sesame legumin-like as well as 2S albumins and oleosin isoforms or selected enzymic proteins. |
| first_indexed | 2024-03-10T13:17:31Z |
| format | Article |
| id | doaj.art-8a990a84f15e447b9b8c0f8b0bb8dce7 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T13:17:31Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-8a990a84f15e447b9b8c0f8b0bb8dce72023-11-21T10:18:50ZengMDPI AGMolecules1420-30492021-03-01266157710.3390/molecules26061577Multispecies Identification of Oilseed- and Meat-Specific Proteins and Heat-Stable Peptide Markers in Food ProductsKlaudia Kotecka-Majchrzak0Natalia Kasałka-Czarna1Agata Sumara2Emilia Fornal3Magdalena Montowska4Department of Meat Technology, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624 Poznan, PolandDepartment of Meat Technology, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624 Poznan, PolandDepartment of Pathophysiology, Medical University of Lublin, Jaczewskiego 8b, 20-090 Lublin, PolandDepartment of Pathophysiology, Medical University of Lublin, Jaczewskiego 8b, 20-090 Lublin, PolandDepartment of Meat Technology, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624 Poznan, PolandConsumer demand for both plant products and meat products enriched with plant raw materials is constantly increasing. Therefore, new versatile and reliable methods are needed to find and combat fraudulent practices in processed foods. The objective of this study was to identify oilseed species-specific peptide markers and meat-specific markers that were resistant to processing, for multispecies authentication of different meat and vegan food products using the proteomic LC-MS/MS method. To assess the limit of detection (LOD) for hemp proteins, cooked meatballs consisting of three meat species and hemp cake at a final concentration of up to 7.4% were examined. Hemp addition at a low concentration of below 1% was detected. The LOD for edestin subunits and albumin was 0.9% (<i>w</i>/<i>w</i>), whereas for 7S vicilin-like protein it was 4.2% (<i>w</i>/<i>w</i>). Specific heat-stable peptides unique to hemp seeds, flaxseed, nigella, pumpkin, sesame, and sunflower seeds, as well as guinea fowl, rabbit, pork, and chicken meat, were detected in different meat and vegan foods. Most of the oilseed-specific peptides were identified as processing-resistant markers belonging to 11S globulin subunits, namely conlinin, edestin, helianthinin, pumpkin vicilin-like or late embryogenesis proteins, and sesame legumin-like as well as 2S albumins and oleosin isoforms or selected enzymic proteins.https://www.mdpi.com/1420-3049/26/6/1577food authenticityhempsunfloweroilseedsguinea fowlrabbit meat |
| spellingShingle | Klaudia Kotecka-Majchrzak Natalia Kasałka-Czarna Agata Sumara Emilia Fornal Magdalena Montowska Multispecies Identification of Oilseed- and Meat-Specific Proteins and Heat-Stable Peptide Markers in Food Products Molecules food authenticity hemp sunflower oilseeds guinea fowl rabbit meat |
| title | Multispecies Identification of Oilseed- and Meat-Specific Proteins and Heat-Stable Peptide Markers in Food Products |
| title_full | Multispecies Identification of Oilseed- and Meat-Specific Proteins and Heat-Stable Peptide Markers in Food Products |
| title_fullStr | Multispecies Identification of Oilseed- and Meat-Specific Proteins and Heat-Stable Peptide Markers in Food Products |
| title_full_unstemmed | Multispecies Identification of Oilseed- and Meat-Specific Proteins and Heat-Stable Peptide Markers in Food Products |
| title_short | Multispecies Identification of Oilseed- and Meat-Specific Proteins and Heat-Stable Peptide Markers in Food Products |
| title_sort | multispecies identification of oilseed and meat specific proteins and heat stable peptide markers in food products |
| topic | food authenticity hemp sunflower oilseeds guinea fowl rabbit meat |
| url | https://www.mdpi.com/1420-3049/26/6/1577 |
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