In utero intraocular AAV injection for early gene expression in the developing rodent retina

Summary: The visual system is the best system to study activity-dependent sensory circuit development. The connections from the retina to the dorsal lateral geniculate nucleus, the retinogeniculate connections, undergo extensive remodeling during early postnatal life. Thus, techniques that allow the...

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Bibliographic Details
Main Authors: Masahiro Yasuda, Sivapratha Nagappan-Chettiar, Hisashi Umemori
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721004494
Description
Summary:Summary: The visual system is the best system to study activity-dependent sensory circuit development. The connections from the retina to the dorsal lateral geniculate nucleus, the retinogeniculate connections, undergo extensive remodeling during early postnatal life. Thus, techniques that allow the expression of transgenes early in the developing retina are essential to study visual system development. Here, we describe a protocol to express genes-of-interest in the developing mouse retina via in utero intraocular adeno-associated virus injections.For complete details on the use and execution of this protocol, please refer to Yasuda et al. (2021).
ISSN:2666-1667