Sprayed biodegradable liquid film improved the freezing tolerance of cv. Cabernet Sauvignon by up-regulating soluble protein and carbohydrate levels and alleviating oxidative damage
Most cultivars of Vitis vinifera L. are very sensitive to cold. As an exogenous protectant, Biodegradable Liquid Film (BLF) is considered to protect winegrapes from low temperatures and dry winds for safe overwintering. This study aimed to reveal the physiological and biochemical mechanisms of BLF r...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1021483/full |
| _version_ | 1811192071405436928 |
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| author | Xing Han Fei Yao Ting-ting Xue Zhi-lei Wang Ying Wang Xiao Cao Miao Hui Dong Wu Yi-han Li Hua Wang Hua Wang Hua Wang Hua Wang Hua Li Hua Li Hua Li Hua Li |
| author_facet | Xing Han Fei Yao Ting-ting Xue Zhi-lei Wang Ying Wang Xiao Cao Miao Hui Dong Wu Yi-han Li Hua Wang Hua Wang Hua Wang Hua Wang Hua Li Hua Li Hua Li Hua Li |
| author_sort | Xing Han |
| collection | DOAJ |
| description | Most cultivars of Vitis vinifera L. are very sensitive to cold. As an exogenous protectant, Biodegradable Liquid Film (BLF) is considered to protect winegrapes from low temperatures and dry winds for safe overwintering. This study aimed to reveal the physiological and biochemical mechanisms of BLF regulating the freezing tolerance of wine grapes. Groups of ten-year-old vines (Cabernet Sauvignon) were sprayed with BLF in November 2020 and 2021, or left untreated as a control treatment, and field plant mortality after overwintering were investigated. Branch samples were collected monthly for determination of biochemical indicators. Dormant two-year-old cuttings (Cabernet Sauvignon) were also used for the determination of relative expression levels of key genes. The results showed that the application of BLF reduced the branch semi-lethal temperature in January and February samples compared with control, and reduced the mortality of above-ground parts, branches and buds. The physiological status of shoots was greatly affected by the climatic conditions of the year, but BLF treatment increased the levels of soluble protein and soluble sugar, and also decreased the content of superoxide anion and malondialdehyde at most sampling times. Correlation analysis showed that the differences in freezing tolerance between BLF and no treated overwintering(CK) vines were mainly related to peroxidase activity, soluble sugar, reducing sugar and starch content. Low temperature stress activated the over expression of ICE1, CBF1, and CBF3, especially for 12h. BLF treatment significantly increased the expression levels of CBF1 and CBF3 under low temperature stress. Overall, these results demonstrate that BLF treatment protects vines from freezing damage by upregulating osmo-regulatory substances and alleviating oxidative damage. |
| first_indexed | 2024-04-11T23:46:49Z |
| format | Article |
| id | doaj.art-8a9ae7c5e77d49b8814f6bb255b82ca8 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T23:46:49Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-8a9ae7c5e77d49b8814f6bb255b82ca82022-12-22T03:56:38ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-11-011310.3389/fpls.2022.10214831021483Sprayed biodegradable liquid film improved the freezing tolerance of cv. Cabernet Sauvignon by up-regulating soluble protein and carbohydrate levels and alleviating oxidative damageXing Han0Fei Yao1Ting-ting Xue2Zhi-lei Wang3Ying Wang4Xiao Cao5Miao Hui6Dong Wu7Yi-han Li8Hua Wang9Hua Wang10Hua Wang11Hua Wang12Hua Li13Hua Li14Hua Li15Hua Li16College of Enology, Northwest A & F University, Yangling, ChinaCollege of Enology, Northwest A & F University, Yangling, ChinaSchool of Wine, Ningxia University, Yinchuan, ChinaCollege of Enology, Northwest A & F University, Yangling, ChinaCollege of Enology, Northwest A & F University, Yangling, ChinaCollege of Enology, Northwest A & F University, Yangling, ChinaCollege of Enology, Northwest A & F University, Yangling, ChinaCollege of Enology, Northwest A & F University, Yangling, ChinaCollege of Enology, Northwest A & F University, Yangling, ChinaCollege of Enology, Northwest A & F University, Yangling, ChinaChina Wine Industry Technology Institute, Yinchuan, ChinaShaanxi Engineering Research Center for Viti-Viniculture, Yangling, ChinaEngineering Research Center for Viti-Viniculture, National Forestry and Grassland Administration, Yangling, ChinaCollege of Enology, Northwest A & F University, Yangling, ChinaChina Wine Industry Technology Institute, Yinchuan, ChinaShaanxi Engineering Research Center for Viti-Viniculture, Yangling, ChinaEngineering Research Center for Viti-Viniculture, National Forestry and Grassland Administration, Yangling, ChinaMost cultivars of Vitis vinifera L. are very sensitive to cold. As an exogenous protectant, Biodegradable Liquid Film (BLF) is considered to protect winegrapes from low temperatures and dry winds for safe overwintering. This study aimed to reveal the physiological and biochemical mechanisms of BLF regulating the freezing tolerance of wine grapes. Groups of ten-year-old vines (Cabernet Sauvignon) were sprayed with BLF in November 2020 and 2021, or left untreated as a control treatment, and field plant mortality after overwintering were investigated. Branch samples were collected monthly for determination of biochemical indicators. Dormant two-year-old cuttings (Cabernet Sauvignon) were also used for the determination of relative expression levels of key genes. The results showed that the application of BLF reduced the branch semi-lethal temperature in January and February samples compared with control, and reduced the mortality of above-ground parts, branches and buds. The physiological status of shoots was greatly affected by the climatic conditions of the year, but BLF treatment increased the levels of soluble protein and soluble sugar, and also decreased the content of superoxide anion and malondialdehyde at most sampling times. Correlation analysis showed that the differences in freezing tolerance between BLF and no treated overwintering(CK) vines were mainly related to peroxidase activity, soluble sugar, reducing sugar and starch content. Low temperature stress activated the over expression of ICE1, CBF1, and CBF3, especially for 12h. BLF treatment significantly increased the expression levels of CBF1 and CBF3 under low temperature stress. Overall, these results demonstrate that BLF treatment protects vines from freezing damage by upregulating osmo-regulatory substances and alleviating oxidative damage.https://www.frontiersin.org/articles/10.3389/fpls.2022.1021483/fullbiodegradable liquid film (BLF)low temperature stressfreezing tolerancemortalityantioxidant systemosmoregulation |
| spellingShingle | Xing Han Fei Yao Ting-ting Xue Zhi-lei Wang Ying Wang Xiao Cao Miao Hui Dong Wu Yi-han Li Hua Wang Hua Wang Hua Wang Hua Wang Hua Li Hua Li Hua Li Hua Li Sprayed biodegradable liquid film improved the freezing tolerance of cv. Cabernet Sauvignon by up-regulating soluble protein and carbohydrate levels and alleviating oxidative damage Frontiers in Plant Science biodegradable liquid film (BLF) low temperature stress freezing tolerance mortality antioxidant system osmoregulation |
| title | Sprayed biodegradable liquid film improved the freezing tolerance of cv. Cabernet Sauvignon by up-regulating soluble protein and carbohydrate levels and alleviating oxidative damage |
| title_full | Sprayed biodegradable liquid film improved the freezing tolerance of cv. Cabernet Sauvignon by up-regulating soluble protein and carbohydrate levels and alleviating oxidative damage |
| title_fullStr | Sprayed biodegradable liquid film improved the freezing tolerance of cv. Cabernet Sauvignon by up-regulating soluble protein and carbohydrate levels and alleviating oxidative damage |
| title_full_unstemmed | Sprayed biodegradable liquid film improved the freezing tolerance of cv. Cabernet Sauvignon by up-regulating soluble protein and carbohydrate levels and alleviating oxidative damage |
| title_short | Sprayed biodegradable liquid film improved the freezing tolerance of cv. Cabernet Sauvignon by up-regulating soluble protein and carbohydrate levels and alleviating oxidative damage |
| title_sort | sprayed biodegradable liquid film improved the freezing tolerance of cv cabernet sauvignon by up regulating soluble protein and carbohydrate levels and alleviating oxidative damage |
| topic | biodegradable liquid film (BLF) low temperature stress freezing tolerance mortality antioxidant system osmoregulation |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1021483/full |
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