Two RNAs or DNAs May Artificially Fuse Together at a Short Homologous Sequence (SHS) during Reverse Transcription or Polymerase Chain Reactions, and Thus Reporting an SHS-Containing Chimeric RNA Requires Extra Caution.

Two RNAs or DNAs May Artificially Fuse Together at a Short Homologous Sequence (SHS) during Reverse Transcription or Polymerase Chain Reactions, and Thus Reporting an SHS-Containing Chimeric RNA Requires Extra Caution.

Tens of thousands of chimeric RNAs have been reported. Most of them contain a short homologous sequence (SHS) at the joining site of the two partner genes but are not associated with a fusion gene. We hypothesize that many of these chimeras may be technical artifacts derived from SHS-caused mis-prim...

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Bibliografiske detaljer
Main Authors:	Bingkun Xie, Wei Yang, Yongchang Ouyang, Lichan Chen, Hesheng Jiang, Yuying Liao, D Joshua Liao
Format:	Article
Sprog:	English
Udgivet:	Public Library of Science (PLoS) 2016-01-01
Serier:	PLoS ONE
Online adgang:	http://europepmc.org/articles/PMC4858267?pdf=render

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