Formulation and in-vitro preliminary screening of polyphyto antilithiatic combination
Objective: To study people suffered from urinary stone problem by calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD) containing stones. Methods: In the present study, CO crystals were grown by mixing and stirring two equal volumes of solutions A and B. Two solutions of following c...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2013-01-01
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| Series: | Journal of Acute Disease |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2221618913601298 |
| _version_ | 1818233859230138368 |
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| author | Abhishek Bharadwaj Kumud Upadhayaya N.V. Satheesh Madhav |
| author_facet | Abhishek Bharadwaj Kumud Upadhayaya N.V. Satheesh Madhav |
| author_sort | Abhishek Bharadwaj |
| collection | DOAJ |
| description | Objective: To study people suffered from urinary stone problem by calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD) containing stones.
Methods: In the present study, CO crystals were grown by mixing and stirring two equal volumes of solutions A and B. Two solutions of following composition were mixed: A: Na2C2O4(2 mmol) and B: CaCl2·2H2O (10 mmol). The crystals after incubation in tris buffer were then subjected to the aqueous extract of the polyherbal formulation and cystone was taken as standard.
Results: After the treatment with the extract the crystals were microscopically examined and elemental analysis for calcium was carried out. The formulation proved out to be very effective against the CO crystals.
Conclusions: The results have come out to be very motivating and further pharmacological study could be carried out on the samples to reveal an effective drug for the urolithiasis. Data reveals that phytotherapeutic agents could be useful as either an alternative or a complementary therapy in the management of urolithiasis. |
| first_indexed | 2024-12-12T11:28:52Z |
| format | Article |
| id | doaj.art-8aa310ed7df3470194be21f68803d129 |
| institution | Directory Open Access Journal |
| issn | 2221-6189 |
| language | English |
| last_indexed | 2024-12-12T11:28:52Z |
| publishDate | 2013-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Acute Disease |
| spelling | doaj.art-8aa310ed7df3470194be21f68803d1292022-12-22T00:25:51ZengWolters Kluwer Medknow PublicationsJournal of Acute Disease2221-61892013-01-012321421710.1016/S2221-6189(13)60129-8Formulation and in-vitro preliminary screening of polyphyto antilithiatic combinationAbhishek Bharadwaj0Kumud Upadhayaya1N.V. Satheesh Madhav2Department of Pharmacognosy, Himachal Institute of Pharmacy, Paonta Sahib (HP), IndiaDepartment of Pharmacognosy, Kumaun University, Nanital Bhimtal, (UK), IndiaDepartment of Pharmaceutics, Dehradun Institute of Technology, Dehradun (UK), IndiaObjective: To study people suffered from urinary stone problem by calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD) containing stones. Methods: In the present study, CO crystals were grown by mixing and stirring two equal volumes of solutions A and B. Two solutions of following composition were mixed: A: Na2C2O4(2 mmol) and B: CaCl2·2H2O (10 mmol). The crystals after incubation in tris buffer were then subjected to the aqueous extract of the polyherbal formulation and cystone was taken as standard. Results: After the treatment with the extract the crystals were microscopically examined and elemental analysis for calcium was carried out. The formulation proved out to be very effective against the CO crystals. Conclusions: The results have come out to be very motivating and further pharmacological study could be carried out on the samples to reveal an effective drug for the urolithiasis. Data reveals that phytotherapeutic agents could be useful as either an alternative or a complementary therapy in the management of urolithiasis.http://www.sciencedirect.com/science/article/pii/S2221618913601298Calcium oxalateCrystallizationCalculiFormulation |
| spellingShingle | Abhishek Bharadwaj Kumud Upadhayaya N.V. Satheesh Madhav Formulation and in-vitro preliminary screening of polyphyto antilithiatic combination Journal of Acute Disease Calcium oxalate Crystallization Calculi Formulation |
| title | Formulation and in-vitro preliminary screening of polyphyto antilithiatic combination |
| title_full | Formulation and in-vitro preliminary screening of polyphyto antilithiatic combination |
| title_fullStr | Formulation and in-vitro preliminary screening of polyphyto antilithiatic combination |
| title_full_unstemmed | Formulation and in-vitro preliminary screening of polyphyto antilithiatic combination |
| title_short | Formulation and in-vitro preliminary screening of polyphyto antilithiatic combination |
| title_sort | formulation and in vitro preliminary screening of polyphyto antilithiatic combination |
| topic | Calcium oxalate Crystallization Calculi Formulation |
| url | http://www.sciencedirect.com/science/article/pii/S2221618913601298 |
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