Virus-Mediated Targeted DNA Methylation Illuminates the Dynamics of Methylation in an Endogenous Plant Gene
DNA methylation maintains genome stability and regulates gene expression in plants. RNA-directed DNA methylation (RdDM) is critical for appropriate methylation. However, no efficient tools are available for the investigation of the functions of specific DNA methylation. In this study, the cucumber m...
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| Format: | Article |
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MDPI AG
2021-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/8/4125 |
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| author | Go Atsumi Kouki Matsuo Noriho Fukuzawa Takeshi Matsumura |
| author_facet | Go Atsumi Kouki Matsuo Noriho Fukuzawa Takeshi Matsumura |
| author_sort | Go Atsumi |
| collection | DOAJ |
| description | DNA methylation maintains genome stability and regulates gene expression in plants. RNA-directed DNA methylation (RdDM) is critical for appropriate methylation. However, no efficient tools are available for the investigation of the functions of specific DNA methylation. In this study, the cucumber mosaic virus vector was used for targeted DNA methylation. Methylation was rapidly induced but gradually decreased from the 3′ end of the target endogenous sequence in <i>Nicotiana benthamiana</i>, suggesting a mechanism to protect against the ectopic introduction of DNA methylation. Increasing 24-nt siRNAs blocked this reduction in methylation by down-regulating <i>DCL2</i> and <i>DCL4</i>. RdDM relies on the sequence identity between RNA and genomic DNA; however, this identity does not appear to be the sole determinant for efficient DNA methylation. The current findings provide new insight into the regulation of DNA methylation and promote additional effort to develop efficient targeted DNA methylation in plants. |
| first_indexed | 2024-03-10T12:14:34Z |
| format | Article |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T12:14:34Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8aace2a84bf841528e391770a89bcf642023-11-21T15:56:04ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-04-01228412510.3390/ijms22084125Virus-Mediated Targeted DNA Methylation Illuminates the Dynamics of Methylation in an Endogenous Plant GeneGo Atsumi0Kouki Matsuo1Noriho Fukuzawa2Takeshi Matsumura3Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Sapporo, Hokkaido 062-8517, JapanBioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Sapporo, Hokkaido 062-8517, JapanBioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Sapporo, Hokkaido 062-8517, JapanBioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Sapporo, Hokkaido 062-8517, JapanDNA methylation maintains genome stability and regulates gene expression in plants. RNA-directed DNA methylation (RdDM) is critical for appropriate methylation. However, no efficient tools are available for the investigation of the functions of specific DNA methylation. In this study, the cucumber mosaic virus vector was used for targeted DNA methylation. Methylation was rapidly induced but gradually decreased from the 3′ end of the target endogenous sequence in <i>Nicotiana benthamiana</i>, suggesting a mechanism to protect against the ectopic introduction of DNA methylation. Increasing 24-nt siRNAs blocked this reduction in methylation by down-regulating <i>DCL2</i> and <i>DCL4</i>. RdDM relies on the sequence identity between RNA and genomic DNA; however, this identity does not appear to be the sole determinant for efficient DNA methylation. The current findings provide new insight into the regulation of DNA methylation and promote additional effort to develop efficient targeted DNA methylation in plants.https://www.mdpi.com/1422-0067/22/8/4125DNA methylationvirus vectorRdDMtranscriptional gene silencing |
| spellingShingle | Go Atsumi Kouki Matsuo Noriho Fukuzawa Takeshi Matsumura Virus-Mediated Targeted DNA Methylation Illuminates the Dynamics of Methylation in an Endogenous Plant Gene International Journal of Molecular Sciences DNA methylation virus vector RdDM transcriptional gene silencing |
| title | Virus-Mediated Targeted DNA Methylation Illuminates the Dynamics of Methylation in an Endogenous Plant Gene |
| title_full | Virus-Mediated Targeted DNA Methylation Illuminates the Dynamics of Methylation in an Endogenous Plant Gene |
| title_fullStr | Virus-Mediated Targeted DNA Methylation Illuminates the Dynamics of Methylation in an Endogenous Plant Gene |
| title_full_unstemmed | Virus-Mediated Targeted DNA Methylation Illuminates the Dynamics of Methylation in an Endogenous Plant Gene |
| title_short | Virus-Mediated Targeted DNA Methylation Illuminates the Dynamics of Methylation in an Endogenous Plant Gene |
| title_sort | virus mediated targeted dna methylation illuminates the dynamics of methylation in an endogenous plant gene |
| topic | DNA methylation virus vector RdDM transcriptional gene silencing |
| url | https://www.mdpi.com/1422-0067/22/8/4125 |
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