Promoting the Calcium-Uptake Bioactivity of Casein Phosphopeptides in vitro and in vivo
Casein phosphopeptides have been studied widely for their ability to chelate calcium. However, systematic studies on the effects of casein phosphopeptides (CPP) on calcium absorption in vitro and in vivo are scarce. The purities of two commercially available products, CPP1 and CPP2, are 18.37 and 25...
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Frontiers Media S.A.
2021-08-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2021.743791/full |
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| author | Guo Liu Guo Liu Baoyan Guo Baoyan Guo Shengwei Sun Minna Luo Fei Liu Jianyin Miao Jian Tang Yahui Huang Yong Cao Mingyue Song |
| author_facet | Guo Liu Guo Liu Baoyan Guo Baoyan Guo Shengwei Sun Minna Luo Fei Liu Jianyin Miao Jian Tang Yahui Huang Yong Cao Mingyue Song |
| author_sort | Guo Liu |
| collection | DOAJ |
| description | Casein phosphopeptides have been studied widely for their ability to chelate calcium. However, systematic studies on the effects of casein phosphopeptides (CPP) on calcium absorption in vitro and in vivo are scarce. The purities of two commercially available products, CPP1 and CPP2, are 18.37 and 25.12%, respectively. Here, the in vitro calcium binding capacity of CPP2 was 142.56 ± 7.39 mg/g, which was higher than that of CPP1 (107.15 ± 6.27 mg/g). The calcium transport results in a Caco-2 monolayer model indicated that, relative to controls, CPP1 and CPP2 increased calcium transport by 21.78 and 53.68%, respectively. Subsequent animal experiments showed that the CPP2-Ca-H group (1% Ca, 0.4% CPP2) had significant increases in the femur index, serum Ca2+ and serum osteocalcin levels, and femoral Ca content. The CPP2-Ca-H animal also had decreased serum alkaline phosphatase levels, parathyroid hormone content, and urinary pyridinoline content. Overall, our results demonstrated that CPP2 had stronger effects on promoting calcium uptake than CPP1. |
| first_indexed | 2024-12-16T11:38:07Z |
| format | Article |
| id | doaj.art-8aadc3d32fa44954ba6ddcaa6418375f |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-12-16T11:38:07Z |
| publishDate | 2021-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-8aadc3d32fa44954ba6ddcaa6418375f2022-12-21T22:33:00ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2021-08-01810.3389/fnut.2021.743791743791Promoting the Calcium-Uptake Bioactivity of Casein Phosphopeptides in vitro and in vivoGuo Liu0Guo Liu1Baoyan Guo2Baoyan Guo3Shengwei Sun4Minna Luo5Fei Liu6Jianyin Miao7Jian Tang8Yahui Huang9Yong Cao10Mingyue Song11Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, ChinaCollege of Horticulture, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, ChinaCollege of Horticulture, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, ChinaGuangzhou Greencream Biotech Co., Ltd., Guangzhou, ChinaGuangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, ChinaInfinitus (China) Company Ltd., Guangzhou, ChinaCollege of Horticulture, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, ChinaCasein phosphopeptides have been studied widely for their ability to chelate calcium. However, systematic studies on the effects of casein phosphopeptides (CPP) on calcium absorption in vitro and in vivo are scarce. The purities of two commercially available products, CPP1 and CPP2, are 18.37 and 25.12%, respectively. Here, the in vitro calcium binding capacity of CPP2 was 142.56 ± 7.39 mg/g, which was higher than that of CPP1 (107.15 ± 6.27 mg/g). The calcium transport results in a Caco-2 monolayer model indicated that, relative to controls, CPP1 and CPP2 increased calcium transport by 21.78 and 53.68%, respectively. Subsequent animal experiments showed that the CPP2-Ca-H group (1% Ca, 0.4% CPP2) had significant increases in the femur index, serum Ca2+ and serum osteocalcin levels, and femoral Ca content. The CPP2-Ca-H animal also had decreased serum alkaline phosphatase levels, parathyroid hormone content, and urinary pyridinoline content. Overall, our results demonstrated that CPP2 had stronger effects on promoting calcium uptake than CPP1.https://www.frontiersin.org/articles/10.3389/fnut.2021.743791/fullcasein phosphopeptidescalcium binding capacitycalcium uptake bioactivitiesCaco-2animal model |
| spellingShingle | Guo Liu Guo Liu Baoyan Guo Baoyan Guo Shengwei Sun Minna Luo Fei Liu Jianyin Miao Jian Tang Yahui Huang Yong Cao Mingyue Song Promoting the Calcium-Uptake Bioactivity of Casein Phosphopeptides in vitro and in vivo Frontiers in Nutrition casein phosphopeptides calcium binding capacity calcium uptake bioactivities Caco-2 animal model |
| title | Promoting the Calcium-Uptake Bioactivity of Casein Phosphopeptides in vitro and in vivo |
| title_full | Promoting the Calcium-Uptake Bioactivity of Casein Phosphopeptides in vitro and in vivo |
| title_fullStr | Promoting the Calcium-Uptake Bioactivity of Casein Phosphopeptides in vitro and in vivo |
| title_full_unstemmed | Promoting the Calcium-Uptake Bioactivity of Casein Phosphopeptides in vitro and in vivo |
| title_short | Promoting the Calcium-Uptake Bioactivity of Casein Phosphopeptides in vitro and in vivo |
| title_sort | promoting the calcium uptake bioactivity of casein phosphopeptides in vitro and in vivo |
| topic | casein phosphopeptides calcium binding capacity calcium uptake bioactivities Caco-2 animal model |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2021.743791/full |
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