Hydroxyapatite Thin Films of Marine Origin as Sustainable Candidates for Dental Implants
Novel biomaterials with promising bone regeneration potential, derived from rich, renewable, and cheap sources, are reported. Thus, thin films were synthesized from marine-derived (i.e., from fish bones and seashells) hydroxyapatite (MdHA) by pulsed laser deposition (PLD) technique. Besides the phys...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/4/1294 |
| _version_ | 1797603814762610688 |
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| author | Gabriela Dorcioman Valentina Grumezescu George E. Stan Mariana Carmen Chifiriuc Gratiela Pircalabioru Gradisteanu Florin Miculescu Elena Matei Gianina Popescu-Pelin Irina Zgura Valentin Craciun Faik Nüzhet Oktar Liviu Duta |
| author_facet | Gabriela Dorcioman Valentina Grumezescu George E. Stan Mariana Carmen Chifiriuc Gratiela Pircalabioru Gradisteanu Florin Miculescu Elena Matei Gianina Popescu-Pelin Irina Zgura Valentin Craciun Faik Nüzhet Oktar Liviu Duta |
| author_sort | Gabriela Dorcioman |
| collection | DOAJ |
| description | Novel biomaterials with promising bone regeneration potential, derived from rich, renewable, and cheap sources, are reported. Thus, thin films were synthesized from marine-derived (i.e., from fish bones and seashells) hydroxyapatite (MdHA) by pulsed laser deposition (PLD) technique. Besides the physical–chemical and mechanical investigations, the deposited thin films were also evaluated in vitro using dedicated cytocompatibility and antimicrobial assays. The morphological examination of MdHA films revealed the fabrication of rough surfaces, which were shown to favor good cell adhesion, and furthermore could foster the in-situ anchorage of implants. The strong hydrophilic behavior of the thin films was evidenced by contact angle (CA) measurements, with values in the range of 15–18°. The inferred bonding strength adherence values were superior (i.e., ~49 MPa) to the threshold established by ISO regulation for high-load implant coatings. After immersion in biological fluids, the growth of an apatite-based layer was noted, which indicated the good mineralization capacity of the MdHA films. All PLD films exhibited low cytotoxicity on osteoblast, fibroblast, and epithelial cells. Moreover, a persistent protective effect against bacterial and fungal colonization (i.e., 1- to 3-log reduction of <i>E. coli</i>, <i>E. faecalis,</i> and <i>C. albicans</i> growth) was demonstrated after 48 h of incubation, with respect to the Ti control. The good cytocompatibility and effective antimicrobial activity, along with the reduced fabrication costs from sustainable sources (available in large quantities), should, therefore, recommend the MdHA materials proposed herein as innovative and viable solutions for the development of novel coatings for metallic dental implants. |
| first_indexed | 2024-03-11T04:37:24Z |
| format | Article |
| id | doaj.art-8aaea75a792a4a52b3989406bdd97a4c |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-11T04:37:24Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-8aaea75a792a4a52b3989406bdd97a4c2023-11-17T20:55:30ZengMDPI AGPharmaceutics1999-49232023-04-01154129410.3390/pharmaceutics15041294Hydroxyapatite Thin Films of Marine Origin as Sustainable Candidates for Dental ImplantsGabriela Dorcioman0Valentina Grumezescu1George E. Stan2Mariana Carmen Chifiriuc3Gratiela Pircalabioru Gradisteanu4Florin Miculescu5Elena Matei6Gianina Popescu-Pelin7Irina Zgura8Valentin Craciun9Faik Nüzhet Oktar10Liviu Duta11Lasers Department, National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, RomaniaLasers Department, National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, RomaniaNational Institute of Materials Physics, 077125 Magurele, RomaniaDepartment of Microbiology, Faculty of Biology, University of Bucharest, 060101 Bucharest, RomaniaEarth, Environmental and Life Sciences Division, Research Institute of the University of Bucharest (ICUB), 060101 Bucharest, RomaniaFaculty of Materials Science and Engineering, Politehnica University of Bucharest, 060042 Bucharest, RomaniaNational Institute of Materials Physics, 077125 Magurele, RomaniaLasers Department, National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, RomaniaNational Institute of Materials Physics, 077125 Magurele, RomaniaLasers Department, National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, RomaniaDepartment of Bioengineering, Faculty of Engineering, University of Marmara, 34722 Istanbul, TurkeyLasers Department, National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, RomaniaNovel biomaterials with promising bone regeneration potential, derived from rich, renewable, and cheap sources, are reported. Thus, thin films were synthesized from marine-derived (i.e., from fish bones and seashells) hydroxyapatite (MdHA) by pulsed laser deposition (PLD) technique. Besides the physical–chemical and mechanical investigations, the deposited thin films were also evaluated in vitro using dedicated cytocompatibility and antimicrobial assays. The morphological examination of MdHA films revealed the fabrication of rough surfaces, which were shown to favor good cell adhesion, and furthermore could foster the in-situ anchorage of implants. The strong hydrophilic behavior of the thin films was evidenced by contact angle (CA) measurements, with values in the range of 15–18°. The inferred bonding strength adherence values were superior (i.e., ~49 MPa) to the threshold established by ISO regulation for high-load implant coatings. After immersion in biological fluids, the growth of an apatite-based layer was noted, which indicated the good mineralization capacity of the MdHA films. All PLD films exhibited low cytotoxicity on osteoblast, fibroblast, and epithelial cells. Moreover, a persistent protective effect against bacterial and fungal colonization (i.e., 1- to 3-log reduction of <i>E. coli</i>, <i>E. faecalis,</i> and <i>C. albicans</i> growth) was demonstrated after 48 h of incubation, with respect to the Ti control. The good cytocompatibility and effective antimicrobial activity, along with the reduced fabrication costs from sustainable sources (available in large quantities), should, therefore, recommend the MdHA materials proposed herein as innovative and viable solutions for the development of novel coatings for metallic dental implants.https://www.mdpi.com/1999-4923/15/4/1294marine-derived hydroxyapatite thin filmbiomaterialcytocompatibilityantimicrobial activitydental implantpulsed laser deposition (PLD) |
| spellingShingle | Gabriela Dorcioman Valentina Grumezescu George E. Stan Mariana Carmen Chifiriuc Gratiela Pircalabioru Gradisteanu Florin Miculescu Elena Matei Gianina Popescu-Pelin Irina Zgura Valentin Craciun Faik Nüzhet Oktar Liviu Duta Hydroxyapatite Thin Films of Marine Origin as Sustainable Candidates for Dental Implants Pharmaceutics marine-derived hydroxyapatite thin film biomaterial cytocompatibility antimicrobial activity dental implant pulsed laser deposition (PLD) |
| title | Hydroxyapatite Thin Films of Marine Origin as Sustainable Candidates for Dental Implants |
| title_full | Hydroxyapatite Thin Films of Marine Origin as Sustainable Candidates for Dental Implants |
| title_fullStr | Hydroxyapatite Thin Films of Marine Origin as Sustainable Candidates for Dental Implants |
| title_full_unstemmed | Hydroxyapatite Thin Films of Marine Origin as Sustainable Candidates for Dental Implants |
| title_short | Hydroxyapatite Thin Films of Marine Origin as Sustainable Candidates for Dental Implants |
| title_sort | hydroxyapatite thin films of marine origin as sustainable candidates for dental implants |
| topic | marine-derived hydroxyapatite thin film biomaterial cytocompatibility antimicrobial activity dental implant pulsed laser deposition (PLD) |
| url | https://www.mdpi.com/1999-4923/15/4/1294 |
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