Assessment of sepsis-associated encephalopathy by quantitative magnetic resonance spectroscopy in a rat model of cecal ligation and puncture
Proton magnetic resonance spectroscopy (1H-MRS) is the only non-invasive technique to quantify neurometabolic compounds in the living brain. We used 1H-MRS to evaluate the brain metabolites in a rat model of Sepsis-associated encephalopathy (SAE) established by cecal ligation and puncture (CLP). 36...
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Elsevier
2024-03-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024028676 |
| _version_ | 1797259796495204352 |
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| author | Siqi Liu Zhifeng Liu Gongfa Wu Haoyi Ye Zhihua Wu Zhengfei Yang Shanping Jiang |
| author_facet | Siqi Liu Zhifeng Liu Gongfa Wu Haoyi Ye Zhihua Wu Zhengfei Yang Shanping Jiang |
| author_sort | Siqi Liu |
| collection | DOAJ |
| description | Proton magnetic resonance spectroscopy (1H-MRS) is the only non-invasive technique to quantify neurometabolic compounds in the living brain. We used 1H-MRS to evaluate the brain metabolites in a rat model of Sepsis-associated encephalopathy (SAE) established by cecal ligation and puncture (CLP). 36 male Sprague-Dawley rats were randomly divided into sham and CLP groups. Each group was further divided into three subgroups: subgroup O, subgroup M, and subgroup N. Neurological function assessments were performed on the animals in the subgroup O and subgroup N at 24 h, 48 h, and 72 h. The animals in the subgroup M were examined by magnetic resonance imaging (MRI) at 12 h after CLP. Compared with the sham group, the ratio of N-acetylaspartate (NAA) to creatine (Cr) in the hippocampus was significantly lower in the CLP group. The respective ratios of lactate (Lac), myo-inositol (mIns), glutamate and glutamine (Glx), lipid (Lip), and choline (Cho) to Cr in the CLP group were clearly higher than those in the sham group. Cytochrome c, intimately related to oxidative stress, was elevated in the CLP group. Neurofilament light (NfL) chain and glial fibrillary acidic protein (GFAP) scores in the CLP group were significantly higher than those in the sham group, while zonula occludens-1 (ZO-1) was downregulated. Compared with the sham group, the CLP group displayed higher values of oxygen extraction fraction (OEF), central venous–arterial partial pressure of carbon dioxide (P (cv-a) CO2), and central venous lactate (VLac). In contrast, jugular venous oxygen saturation (SjvO2) declined. In the present study, 1H-MRS could be used to quantitatively assess brain injury in terms of microcirculation disorder, oxidative stress, blood–brain barrier disruption, and glial cell activation through changes in metabolites within brain tissue. |
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| format | Article |
| id | doaj.art-8aaea94937bb4eaaa5fd83f2639537cf |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T23:15:07Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
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| series | Heliyon |
| spelling | doaj.art-8aaea94937bb4eaaa5fd83f2639537cf2024-03-17T07:56:27ZengElsevierHeliyon2405-84402024-03-01105e26836Assessment of sepsis-associated encephalopathy by quantitative magnetic resonance spectroscopy in a rat model of cecal ligation and punctureSiqi Liu0Zhifeng Liu1Gongfa Wu2Haoyi Ye3Zhihua Wu4Zhengfei Yang5Shanping Jiang6Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, 510120, ChinaThe Fourth Affiliated Hospital of Guangzhou Medical University, Guangzhou, 511300, ChinaThe Fourth Affiliated Hospital of Guangzhou Medical University, Guangzhou, 511300, ChinaThe Fourth Affiliated Hospital of Guangzhou Medical University, Guangzhou, 511300, ChinaThe Fourth Affiliated Hospital of Guangzhou Medical University, Guangzhou, 511300, ChinaSun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, 510120, China; Corresponding author. Sun Yat-sen Memorial Hospital of Sun Yat-sen University, 107 Yan Jiang Xi Road, Guangzhou, 510120, China.Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, 510120, China; Corresponding author. Sun Yat-sen Memorial Hospital of Sun Yat-sen University, 107 Yan Jiang Xi Road, Guangzhou, 510120, China.Proton magnetic resonance spectroscopy (1H-MRS) is the only non-invasive technique to quantify neurometabolic compounds in the living brain. We used 1H-MRS to evaluate the brain metabolites in a rat model of Sepsis-associated encephalopathy (SAE) established by cecal ligation and puncture (CLP). 36 male Sprague-Dawley rats were randomly divided into sham and CLP groups. Each group was further divided into three subgroups: subgroup O, subgroup M, and subgroup N. Neurological function assessments were performed on the animals in the subgroup O and subgroup N at 24 h, 48 h, and 72 h. The animals in the subgroup M were examined by magnetic resonance imaging (MRI) at 12 h after CLP. Compared with the sham group, the ratio of N-acetylaspartate (NAA) to creatine (Cr) in the hippocampus was significantly lower in the CLP group. The respective ratios of lactate (Lac), myo-inositol (mIns), glutamate and glutamine (Glx), lipid (Lip), and choline (Cho) to Cr in the CLP group were clearly higher than those in the sham group. Cytochrome c, intimately related to oxidative stress, was elevated in the CLP group. Neurofilament light (NfL) chain and glial fibrillary acidic protein (GFAP) scores in the CLP group were significantly higher than those in the sham group, while zonula occludens-1 (ZO-1) was downregulated. Compared with the sham group, the CLP group displayed higher values of oxygen extraction fraction (OEF), central venous–arterial partial pressure of carbon dioxide (P (cv-a) CO2), and central venous lactate (VLac). In contrast, jugular venous oxygen saturation (SjvO2) declined. In the present study, 1H-MRS could be used to quantitatively assess brain injury in terms of microcirculation disorder, oxidative stress, blood–brain barrier disruption, and glial cell activation through changes in metabolites within brain tissue.http://www.sciencedirect.com/science/article/pii/S2405844024028676SepsisCecal ligation and punctureBrain injury1H-MRSNeurological deficit |
| spellingShingle | Siqi Liu Zhifeng Liu Gongfa Wu Haoyi Ye Zhihua Wu Zhengfei Yang Shanping Jiang Assessment of sepsis-associated encephalopathy by quantitative magnetic resonance spectroscopy in a rat model of cecal ligation and puncture Heliyon Sepsis Cecal ligation and puncture Brain injury 1H-MRS Neurological deficit |
| title | Assessment of sepsis-associated encephalopathy by quantitative magnetic resonance spectroscopy in a rat model of cecal ligation and puncture |
| title_full | Assessment of sepsis-associated encephalopathy by quantitative magnetic resonance spectroscopy in a rat model of cecal ligation and puncture |
| title_fullStr | Assessment of sepsis-associated encephalopathy by quantitative magnetic resonance spectroscopy in a rat model of cecal ligation and puncture |
| title_full_unstemmed | Assessment of sepsis-associated encephalopathy by quantitative magnetic resonance spectroscopy in a rat model of cecal ligation and puncture |
| title_short | Assessment of sepsis-associated encephalopathy by quantitative magnetic resonance spectroscopy in a rat model of cecal ligation and puncture |
| title_sort | assessment of sepsis associated encephalopathy by quantitative magnetic resonance spectroscopy in a rat model of cecal ligation and puncture |
| topic | Sepsis Cecal ligation and puncture Brain injury 1H-MRS Neurological deficit |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024028676 |
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