Identification of a Solanum pennellii Chromosome 4 Fruit Flavor and Nutritional Quality-Associated Metabolite QTL
A major resource for tomato quality improvement and gene discovery is the collection of introgression lines (ILs) of cultivated Solanum lycopersicum that contain different, defined chromosomal segments derived from the wild tomato relative, S. pennellii. Among these lines, IL4-4, in which the bottom...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01671/full |
| _version_ | 1818319226190954496 |
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| author | ZHONGYUAN LIU Saleh Alseek Yariv Brotman Yariv Brotman Yi Zheng Zhangjun Fei Denise Tieman James Giovannoni Alisdair Fernie Harry Klee |
| author_facet | ZHONGYUAN LIU Saleh Alseek Yariv Brotman Yariv Brotman Yi Zheng Zhangjun Fei Denise Tieman James Giovannoni Alisdair Fernie Harry Klee |
| author_sort | ZHONGYUAN LIU |
| collection | DOAJ |
| description | A major resource for tomato quality improvement and gene discovery is the collection of introgression lines (ILs) of cultivated Solanum lycopersicum that contain different, defined chromosomal segments derived from the wild tomato relative, S. pennellii. Among these lines, IL4-4, in which the bottom of S. lycopersicum (cv. M82) chromosome 4 is replaced by the corresponding S. pennellii segment, is altered in many primary and secondary metabolites, including many related to fruit flavor and nutritional quality. Here, we provide a comprehensive profile of IL4-4 ripe fruit metabolites, the transcriptome and fine mapping of sub-ILs. Remarkably, out of 327 quantified metabolites, 185 were significantly changed in IL4-4 fruit, compared to the control. These altered metabolites include volatile organic compounds, primary and secondary metabolites. Partial least squares enhanced discriminant analysis of the metabolite levels among sub-ILs indicated that a genome region encompassing 20 putative open reading frames is responsible for most of the metabolic changes in IL4-4 fruit. This work provides comprehensive insights into IL4-4 fruit biochemistry, identifying a small region of the genome that has major effects on a large and diverse set of metabolites. |
| first_indexed | 2024-12-13T10:05:45Z |
| format | Article |
| id | doaj.art-8aaf9066689e4decbb6b78ab6d5cc3c4 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-13T10:05:45Z |
| publishDate | 2016-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-8aaf9066689e4decbb6b78ab6d5cc3c42022-12-21T23:51:33ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-11-01710.3389/fpls.2016.01671227350Identification of a Solanum pennellii Chromosome 4 Fruit Flavor and Nutritional Quality-Associated Metabolite QTLZHONGYUAN LIU0Saleh Alseek1Yariv Brotman2Yariv Brotman3Yi Zheng4Zhangjun Fei5Denise Tieman6James Giovannoni7Alisdair Fernie8Harry Klee9University of FloridaMax-Planck-Institute of Molecular Plant PhysiologyMax-Planck-Institute of Molecular Plant PhysiologyBen Gurion University of the NegevBoyce Thompson InstituteBoyce Thompson InstituteUniversity of FloridaBoyce Thompson InstituteMax-Planck-Institute of Molecular Plant PhysiologyUniversity of FloridaA major resource for tomato quality improvement and gene discovery is the collection of introgression lines (ILs) of cultivated Solanum lycopersicum that contain different, defined chromosomal segments derived from the wild tomato relative, S. pennellii. Among these lines, IL4-4, in which the bottom of S. lycopersicum (cv. M82) chromosome 4 is replaced by the corresponding S. pennellii segment, is altered in many primary and secondary metabolites, including many related to fruit flavor and nutritional quality. Here, we provide a comprehensive profile of IL4-4 ripe fruit metabolites, the transcriptome and fine mapping of sub-ILs. Remarkably, out of 327 quantified metabolites, 185 were significantly changed in IL4-4 fruit, compared to the control. These altered metabolites include volatile organic compounds, primary and secondary metabolites. Partial least squares enhanced discriminant analysis of the metabolite levels among sub-ILs indicated that a genome region encompassing 20 putative open reading frames is responsible for most of the metabolic changes in IL4-4 fruit. This work provides comprehensive insights into IL4-4 fruit biochemistry, identifying a small region of the genome that has major effects on a large and diverse set of metabolites.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01671/fullMetabolomeTranscription, GeneticflavorFruit ripeningIntrogression |
| spellingShingle | ZHONGYUAN LIU Saleh Alseek Yariv Brotman Yariv Brotman Yi Zheng Zhangjun Fei Denise Tieman James Giovannoni Alisdair Fernie Harry Klee Identification of a Solanum pennellii Chromosome 4 Fruit Flavor and Nutritional Quality-Associated Metabolite QTL Frontiers in Plant Science Metabolome Transcription, Genetic flavor Fruit ripening Introgression |
| title | Identification of a Solanum pennellii Chromosome 4 Fruit Flavor and Nutritional Quality-Associated Metabolite QTL |
| title_full | Identification of a Solanum pennellii Chromosome 4 Fruit Flavor and Nutritional Quality-Associated Metabolite QTL |
| title_fullStr | Identification of a Solanum pennellii Chromosome 4 Fruit Flavor and Nutritional Quality-Associated Metabolite QTL |
| title_full_unstemmed | Identification of a Solanum pennellii Chromosome 4 Fruit Flavor and Nutritional Quality-Associated Metabolite QTL |
| title_short | Identification of a Solanum pennellii Chromosome 4 Fruit Flavor and Nutritional Quality-Associated Metabolite QTL |
| title_sort | identification of a solanum pennellii chromosome 4 fruit flavor and nutritional quality associated metabolite qtl |
| topic | Metabolome Transcription, Genetic flavor Fruit ripening Introgression |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01671/full |
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