Actin assembly and non-muscle myosin activity drive dendrite retraction in an UNC-6/Netrin dependent self-avoidance response.
Dendrite growth is constrained by a self-avoidance response that induces retraction but the downstream pathways that balance these opposing mechanisms are unknown. We have proposed that the diffusible cue UNC-6(Netrin) is captured by UNC-40(DCC) for a short-range interaction with UNC-5 to trigger se...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-06-01
|
| Series: | PLoS Genetics |
| Online Access: | https://doi.org/10.1371/journal.pgen.1008228 |
| _version_ | 1797350886706511872 |
|---|---|
| author | Lakshmi Sundararajan Cody J Smith Joseph D Watson Bryan A Millis Matthew J Tyska David M Miller |
| author_facet | Lakshmi Sundararajan Cody J Smith Joseph D Watson Bryan A Millis Matthew J Tyska David M Miller |
| author_sort | Lakshmi Sundararajan |
| collection | DOAJ |
| description | Dendrite growth is constrained by a self-avoidance response that induces retraction but the downstream pathways that balance these opposing mechanisms are unknown. We have proposed that the diffusible cue UNC-6(Netrin) is captured by UNC-40(DCC) for a short-range interaction with UNC-5 to trigger self-avoidance in the C. elegans PVD neuron. Here we report that the actin-polymerizing proteins UNC-34(Ena/VASP), WSP-1(WASP), UNC-73(Trio), MIG-10(Lamellipodin) and the Arp2/3 complex effect dendrite retraction in the self-avoidance response mediated by UNC-6(Netrin). The paradoxical idea that actin polymerization results in shorter rather than longer dendrites is explained by our finding that NMY-1 (non-muscle myosin II) is necessary for retraction and could therefore mediate this effect in a contractile mechanism. Our results also show that dendrite length is determined by the antagonistic effects on the actin cytoskeleton of separate sets of effectors for retraction mediated by UNC-6(Netrin) versus outgrowth promoted by the DMA-1 receptor. Thus, our findings suggest that the dendrite length depends on an intrinsic mechanism that balances distinct modes of actin assembly for growth versus retraction. |
| first_indexed | 2024-03-08T12:51:35Z |
| format | Article |
| id | doaj.art-8ab496bd455a4d77a12cda1bb3bc593e |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-03-08T12:51:35Z |
| publishDate | 2019-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-8ab496bd455a4d77a12cda1bb3bc593e2024-01-20T05:33:19ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042019-06-01156e100822810.1371/journal.pgen.1008228Actin assembly and non-muscle myosin activity drive dendrite retraction in an UNC-6/Netrin dependent self-avoidance response.Lakshmi SundararajanCody J SmithJoseph D WatsonBryan A MillisMatthew J TyskaDavid M MillerDendrite growth is constrained by a self-avoidance response that induces retraction but the downstream pathways that balance these opposing mechanisms are unknown. We have proposed that the diffusible cue UNC-6(Netrin) is captured by UNC-40(DCC) for a short-range interaction with UNC-5 to trigger self-avoidance in the C. elegans PVD neuron. Here we report that the actin-polymerizing proteins UNC-34(Ena/VASP), WSP-1(WASP), UNC-73(Trio), MIG-10(Lamellipodin) and the Arp2/3 complex effect dendrite retraction in the self-avoidance response mediated by UNC-6(Netrin). The paradoxical idea that actin polymerization results in shorter rather than longer dendrites is explained by our finding that NMY-1 (non-muscle myosin II) is necessary for retraction and could therefore mediate this effect in a contractile mechanism. Our results also show that dendrite length is determined by the antagonistic effects on the actin cytoskeleton of separate sets of effectors for retraction mediated by UNC-6(Netrin) versus outgrowth promoted by the DMA-1 receptor. Thus, our findings suggest that the dendrite length depends on an intrinsic mechanism that balances distinct modes of actin assembly for growth versus retraction.https://doi.org/10.1371/journal.pgen.1008228 |
| spellingShingle | Lakshmi Sundararajan Cody J Smith Joseph D Watson Bryan A Millis Matthew J Tyska David M Miller Actin assembly and non-muscle myosin activity drive dendrite retraction in an UNC-6/Netrin dependent self-avoidance response. PLoS Genetics |
| title | Actin assembly and non-muscle myosin activity drive dendrite retraction in an UNC-6/Netrin dependent self-avoidance response. |
| title_full | Actin assembly and non-muscle myosin activity drive dendrite retraction in an UNC-6/Netrin dependent self-avoidance response. |
| title_fullStr | Actin assembly and non-muscle myosin activity drive dendrite retraction in an UNC-6/Netrin dependent self-avoidance response. |
| title_full_unstemmed | Actin assembly and non-muscle myosin activity drive dendrite retraction in an UNC-6/Netrin dependent self-avoidance response. |
| title_short | Actin assembly and non-muscle myosin activity drive dendrite retraction in an UNC-6/Netrin dependent self-avoidance response. |
| title_sort | actin assembly and non muscle myosin activity drive dendrite retraction in an unc 6 netrin dependent self avoidance response |
| url | https://doi.org/10.1371/journal.pgen.1008228 |
| work_keys_str_mv | AT lakshmisundararajan actinassemblyandnonmusclemyosinactivitydrivedendriteretractioninanunc6netrindependentselfavoidanceresponse AT codyjsmith actinassemblyandnonmusclemyosinactivitydrivedendriteretractioninanunc6netrindependentselfavoidanceresponse AT josephdwatson actinassemblyandnonmusclemyosinactivitydrivedendriteretractioninanunc6netrindependentselfavoidanceresponse AT bryanamillis actinassemblyandnonmusclemyosinactivitydrivedendriteretractioninanunc6netrindependentselfavoidanceresponse AT matthewjtyska actinassemblyandnonmusclemyosinactivitydrivedendriteretractioninanunc6netrindependentselfavoidanceresponse AT davidmmiller actinassemblyandnonmusclemyosinactivitydrivedendriteretractioninanunc6netrindependentselfavoidanceresponse |