Detection of Cassava Component in Sweet Potato Noodles by Real-Time Loop-mediated Isothermal Amplification (Real-time LAMP) Method
Sweet potato (<i>Ipomoea batatas</i>) noodles are a traditional Chinese food with a high nutritional value; however, starch adulteration is a big concern. The objective of this study was to develop a reliable method for the rapid detection of cassava (<i>Manihot esculenta</i>...
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MDPI AG
2019-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/11/2043 |
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| author | Deguo Wang Yongzhen Wang Kai Zhu Lijia Shi Meng Zhang Jianghan Yu Yanhong Liu |
| author_facet | Deguo Wang Yongzhen Wang Kai Zhu Lijia Shi Meng Zhang Jianghan Yu Yanhong Liu |
| author_sort | Deguo Wang |
| collection | DOAJ |
| description | Sweet potato (<i>Ipomoea batatas</i>) noodles are a traditional Chinese food with a high nutritional value; however, starch adulteration is a big concern. The objective of this study was to develop a reliable method for the rapid detection of cassava (<i>Manihot esculenta</i>) components in sweet potato noodles to protect consumers from commercial adulteration. Five specific Loop-mediated Isothermal Amplification (LAMP) primers targeting the internal transcribed spacer (ITS) of cassava were designed, genomic DNA was extracted, the LAMP reaction system was optimized, and the specificity of the primers was verified with genomic DNA of cassava, <i>Ipomoea batatas</i>, <i>Zea mays</i>, and <i>Solanum tuberosum</i>; the detection limit was determined with a serial dilution of adulterated sweet potato starch with cassava starch, and the real-time LAMP method for the detection of the cassava-derived ingredient in sweet potato noodles was established. The results showed that the real-time LAMP method can accurately and specifically detect the cassava component in sweet potato noodles with a detection limit of 1%. Furthermore, the LAMP assay was validated using commercial sweet potato noodle samples, and results showed that 57.7% of sweet potato noodle products (30/52) from retail markets were adulterated with cassava starch in China. This study provides a promising solution for facilitating the surveillance of the commercial adulteration of sweet potato noodles from retail markets. |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-13T13:26:18Z |
| publishDate | 2019-05-01 |
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| series | Molecules |
| spelling | doaj.art-8ab51362827f40f2895c4213320150d12022-12-22T02:45:08ZengMDPI AGMolecules1420-30492019-05-012411204310.3390/molecules24112043molecules24112043Detection of Cassava Component in Sweet Potato Noodles by Real-Time Loop-mediated Isothermal Amplification (Real-time LAMP) MethodDeguo Wang0Yongzhen Wang1Kai Zhu2Lijia Shi3Meng Zhang4Jianghan Yu5Yanhong Liu6Key Laboratory of Biomarker Based Rapid-Detection Technology for Food Safety of Henan Province, Xuchang University, Xuchang 461000, ChinaKey Laboratory of Biomarker Based Rapid-Detection Technology for Food Safety of Henan Province, Xuchang University, Xuchang 461000, ChinaKey Laboratory of Biomarker Based Rapid-Detection Technology for Food Safety of Henan Province, Xuchang University, Xuchang 461000, ChinaKey Laboratory of Biomarker Based Rapid-Detection Technology for Food Safety of Henan Province, Xuchang University, Xuchang 461000, ChinaKey Laboratory of Biomarker Based Rapid-Detection Technology for Food Safety of Henan Province, Xuchang University, Xuchang 461000, ChinaKey Laboratory of Biomarker Based Rapid-Detection Technology for Food Safety of Henan Province, Xuchang University, Xuchang 461000, ChinaMolecular Characterization of Foodborne Pathogens Research Unit, Eastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Wyndmoor, PA 19038, USASweet potato (<i>Ipomoea batatas</i>) noodles are a traditional Chinese food with a high nutritional value; however, starch adulteration is a big concern. The objective of this study was to develop a reliable method for the rapid detection of cassava (<i>Manihot esculenta</i>) components in sweet potato noodles to protect consumers from commercial adulteration. Five specific Loop-mediated Isothermal Amplification (LAMP) primers targeting the internal transcribed spacer (ITS) of cassava were designed, genomic DNA was extracted, the LAMP reaction system was optimized, and the specificity of the primers was verified with genomic DNA of cassava, <i>Ipomoea batatas</i>, <i>Zea mays</i>, and <i>Solanum tuberosum</i>; the detection limit was determined with a serial dilution of adulterated sweet potato starch with cassava starch, and the real-time LAMP method for the detection of the cassava-derived ingredient in sweet potato noodles was established. The results showed that the real-time LAMP method can accurately and specifically detect the cassava component in sweet potato noodles with a detection limit of 1%. Furthermore, the LAMP assay was validated using commercial sweet potato noodle samples, and results showed that 57.7% of sweet potato noodle products (30/52) from retail markets were adulterated with cassava starch in China. This study provides a promising solution for facilitating the surveillance of the commercial adulteration of sweet potato noodles from retail markets.https://www.mdpi.com/1420-3049/24/11/2043loop-mediated isothermal amplification (LAMP)adulterated sweet potato noodlescassava-derived ingredientsinternal transcribed spacer (ITS) |
| spellingShingle | Deguo Wang Yongzhen Wang Kai Zhu Lijia Shi Meng Zhang Jianghan Yu Yanhong Liu Detection of Cassava Component in Sweet Potato Noodles by Real-Time Loop-mediated Isothermal Amplification (Real-time LAMP) Method Molecules loop-mediated isothermal amplification (LAMP) adulterated sweet potato noodles cassava-derived ingredients internal transcribed spacer (ITS) |
| title | Detection of Cassava Component in Sweet Potato Noodles by Real-Time Loop-mediated Isothermal Amplification (Real-time LAMP) Method |
| title_full | Detection of Cassava Component in Sweet Potato Noodles by Real-Time Loop-mediated Isothermal Amplification (Real-time LAMP) Method |
| title_fullStr | Detection of Cassava Component in Sweet Potato Noodles by Real-Time Loop-mediated Isothermal Amplification (Real-time LAMP) Method |
| title_full_unstemmed | Detection of Cassava Component in Sweet Potato Noodles by Real-Time Loop-mediated Isothermal Amplification (Real-time LAMP) Method |
| title_short | Detection of Cassava Component in Sweet Potato Noodles by Real-Time Loop-mediated Isothermal Amplification (Real-time LAMP) Method |
| title_sort | detection of cassava component in sweet potato noodles by real time loop mediated isothermal amplification real time lamp method |
| topic | loop-mediated isothermal amplification (LAMP) adulterated sweet potato noodles cassava-derived ingredients internal transcribed spacer (ITS) |
| url | https://www.mdpi.com/1420-3049/24/11/2043 |
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