Antifungal Activity of Lipopeptides From Bacillus XT1 CECT 8661 Against Botrytis cinerea
This work aims to explore the capacity of a Bacillus methylotrophicus (later heterotypic synonym of Bacillus velezensis) strain named XT1 CECT 8661 against the necrotrophic plant pathogen Botrytis cinerea and to identify the compounds responsible for its activity. Q_TOF electrospray mass spectrometr...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-06-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.01315/full |
| _version_ | 1818949755410055168 |
|---|---|
| author | Laura Toral Miguel Rodríguez Miguel Rodríguez Victoria Béjar Victoria Béjar Inmaculada Sampedro Inmaculada Sampedro |
| author_facet | Laura Toral Miguel Rodríguez Miguel Rodríguez Victoria Béjar Victoria Béjar Inmaculada Sampedro Inmaculada Sampedro |
| author_sort | Laura Toral |
| collection | DOAJ |
| description | This work aims to explore the capacity of a Bacillus methylotrophicus (later heterotypic synonym of Bacillus velezensis) strain named XT1 CECT 8661 against the necrotrophic plant pathogen Botrytis cinerea and to identify the compounds responsible for its activity. Q_TOF electrospray mass spectrometry analysis allows us to detect several lipopeptides – surfactin, bacillomycin, and fengycin – in XT1 cultures. In vitro antibiosis studies demonstrated the efficiency of the lipopeptide fraction for the inhibition of fungal growth. In fact, microscopy studies (SEM/TEM) revealed, an alteration of the morphology of the phytopathogen in interaction with lipopeptides, with resistance structures appearing in the early stages of growth of the fungus. Our studies, carried out with tomatoes, grapes, and strawberries have demonstrated the efficiency of Bacillus XT1 CECT 8661 lipopeptides against B. cinerea infection and it capability to trigger the antioxidant activity in fruit. Overall, the results of this study highlight the potential of lipopeptides of this strain as an effective biological control agent against the colonisation of B. cinerea. |
| first_indexed | 2024-12-20T09:07:44Z |
| format | Article |
| id | doaj.art-8ab614d6003a400b93597290776f9c21 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-20T09:07:44Z |
| publishDate | 2018-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-8ab614d6003a400b93597290776f9c212022-12-21T19:45:40ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-06-01910.3389/fmicb.2018.01315377923Antifungal Activity of Lipopeptides From Bacillus XT1 CECT 8661 Against Botrytis cinereaLaura Toral0Miguel Rodríguez1Miguel Rodríguez2Victoria Béjar3Victoria Béjar4Inmaculada Sampedro5Inmaculada Sampedro6Xtrem Biotech S.L., European Business Innovation Center, Granada, SpainDepartment of Microbiology, Faculty of Pharmacy, University of Granada, Granada, SpainBiomedical Research Center (CIBM), Biotechnology Institute, Granada, SpainDepartment of Microbiology, Faculty of Pharmacy, University of Granada, Granada, SpainBiomedical Research Center (CIBM), Biotechnology Institute, Granada, SpainDepartment of Microbiology, Faculty of Pharmacy, University of Granada, Granada, SpainBiomedical Research Center (CIBM), Biotechnology Institute, Granada, SpainThis work aims to explore the capacity of a Bacillus methylotrophicus (later heterotypic synonym of Bacillus velezensis) strain named XT1 CECT 8661 against the necrotrophic plant pathogen Botrytis cinerea and to identify the compounds responsible for its activity. Q_TOF electrospray mass spectrometry analysis allows us to detect several lipopeptides – surfactin, bacillomycin, and fengycin – in XT1 cultures. In vitro antibiosis studies demonstrated the efficiency of the lipopeptide fraction for the inhibition of fungal growth. In fact, microscopy studies (SEM/TEM) revealed, an alteration of the morphology of the phytopathogen in interaction with lipopeptides, with resistance structures appearing in the early stages of growth of the fungus. Our studies, carried out with tomatoes, grapes, and strawberries have demonstrated the efficiency of Bacillus XT1 CECT 8661 lipopeptides against B. cinerea infection and it capability to trigger the antioxidant activity in fruit. Overall, the results of this study highlight the potential of lipopeptides of this strain as an effective biological control agent against the colonisation of B. cinerea.https://www.frontiersin.org/article/10.3389/fmicb.2018.01315/fullBacillus XT1lipopeptidesantifungal activityBotrytis cinereaantioxidant activity |
| spellingShingle | Laura Toral Miguel Rodríguez Miguel Rodríguez Victoria Béjar Victoria Béjar Inmaculada Sampedro Inmaculada Sampedro Antifungal Activity of Lipopeptides From Bacillus XT1 CECT 8661 Against Botrytis cinerea Frontiers in Microbiology Bacillus XT1 lipopeptides antifungal activity Botrytis cinerea antioxidant activity |
| title | Antifungal Activity of Lipopeptides From Bacillus XT1 CECT 8661 Against Botrytis cinerea |
| title_full | Antifungal Activity of Lipopeptides From Bacillus XT1 CECT 8661 Against Botrytis cinerea |
| title_fullStr | Antifungal Activity of Lipopeptides From Bacillus XT1 CECT 8661 Against Botrytis cinerea |
| title_full_unstemmed | Antifungal Activity of Lipopeptides From Bacillus XT1 CECT 8661 Against Botrytis cinerea |
| title_short | Antifungal Activity of Lipopeptides From Bacillus XT1 CECT 8661 Against Botrytis cinerea |
| title_sort | antifungal activity of lipopeptides from bacillus xt1 cect 8661 against botrytis cinerea |
| topic | Bacillus XT1 lipopeptides antifungal activity Botrytis cinerea antioxidant activity |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.01315/full |
| work_keys_str_mv | AT lauratoral antifungalactivityoflipopeptidesfrombacillusxt1cect8661againstbotrytiscinerea AT miguelrodriguez antifungalactivityoflipopeptidesfrombacillusxt1cect8661againstbotrytiscinerea AT miguelrodriguez antifungalactivityoflipopeptidesfrombacillusxt1cect8661againstbotrytiscinerea AT victoriabejar antifungalactivityoflipopeptidesfrombacillusxt1cect8661againstbotrytiscinerea AT victoriabejar antifungalactivityoflipopeptidesfrombacillusxt1cect8661againstbotrytiscinerea AT inmaculadasampedro antifungalactivityoflipopeptidesfrombacillusxt1cect8661againstbotrytiscinerea AT inmaculadasampedro antifungalactivityoflipopeptidesfrombacillusxt1cect8661againstbotrytiscinerea |