Polyhydroxyalkanoates: Biosynthesis Optimization and Design of Antimicrobial Composites
Background. The accumulation of plastic waste negatively affects the environment and human health. Currently, one of the strategies to address this global ecological problem involves the utilization of biodegradable plastics instead in place of synthetic ones. Among them, polyhydroxyalkanoates (PHA)...
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| Format: | Article |
| Language: | English |
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Igor Sikorsky Kyiv Polytechnic Institute
2023-09-01
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| Series: | Innovative Biosystems and Bioengineering |
| Subjects: | |
| Online Access: | http://ibb.kpi.ua/article/view/280017 |
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| author | Nataliia Koretska Ihor Semeniuk Tetyana Pokynbroda Nataliia Shcheglova Olena Karpenko Andriy Kytsya Vira Lubenets Nataliia Polish |
| author_facet | Nataliia Koretska Ihor Semeniuk Tetyana Pokynbroda Nataliia Shcheglova Olena Karpenko Andriy Kytsya Vira Lubenets Nataliia Polish |
| author_sort | Nataliia Koretska |
| collection | DOAJ |
| description | Background. The accumulation of plastic waste negatively affects the environment and human health. Currently, one of the strategies to address this global ecological problem involves the utilization of biodegradable plastics instead in place of synthetic ones. Among them, polyhydroxyalkanoates (PHA) – microbial intracellular polymers – hold a significant position. Their advantages are biodegradability, biocompatibility, and favorable thermomechanical properties. Given these attributes, PHA has significant prospects for use in medicine, agriculture, and the food industry, in particular for packaging food products.
Objective. Enchance the efficiency of bacterial synthesis of polyhydroxyalkanoates through nutrient media modification, obtain antimicrobial composites based on PHA, and determine their antimicrobial properties.
Methods. The optimization of PHA biosynthesis involved selecting appropriate cultivation conditions, including carbon and nitrogen sources, cultivation time, and working volume. The isolation of PHA from bacterial biomass was achieved through chloroform extraction (mixing for 10 h at 35 °C, with a biomass-to-chloroform ratio of 1:50); followed by precipitation with double the volume of isopropanol. The resulting polymer was then dried to a constant weight at 60 °C. The hydrophobicity of the biopolymer was assessed using the water contact angle measurement. Composites of biopolymers with antimicrobials in the form of films were obtained using two methods: 1) solution casting method; and 2) layering the biocides onto the polymer film. The antimicrobial activity of the resulting composites was determined using the agar diffusion method.
Results. Through the optimization of the mineral media and the change of cultivation conditions, it was possible to obtain 0.26–1.45 g/l of polyhydroxyalkanoates (5.1–34.0% PHA from biomass). The R. ruber UCM Ac-288 strain synthesized the maximum amount of biopolymer (34.0% PHA). This study established the ability of Gordonia bacteria to synthesize PHA for the first time. PHA compositions of optimal content were obtained, with hydrophobicity comparable to that of polyethylene packaging films. Antimicrobial properties of biopolymers composites with biocides have been substantiated.
Conclusions. The bacterial synthesis of PHA was increased by modifying nutrient media. Composites based on PHA with biocides were developed. It was determined that these composites exhibit antimicrobial properties and high hydrophobicity. Consequently, they hold promise for use as biofilms for packaging and preserving food products. |
| first_indexed | 2024-03-08T14:30:13Z |
| format | Article |
| id | doaj.art-8ab6f7b6de104a38a49cabf42235dd8a |
| institution | Directory Open Access Journal |
| issn | 2616-177X |
| language | English |
| last_indexed | 2024-03-08T14:30:13Z |
| publishDate | 2023-09-01 |
| publisher | Igor Sikorsky Kyiv Polytechnic Institute |
| record_format | Article |
| series | Innovative Biosystems and Bioengineering |
| spelling | doaj.art-8ab6f7b6de104a38a49cabf42235dd8a2024-01-12T12:17:15ZengIgor Sikorsky Kyiv Polytechnic InstituteInnovative Biosystems and Bioengineering2616-177X2023-09-0172324110.20535/ibb.2023.7.2.280017318224Polyhydroxyalkanoates: Biosynthesis Optimization and Design of Antimicrobial CompositesNataliia Koretska0Ihor Semeniuk1https://orcid.org/0000-0002-8481-4807Tetyana Pokynbroda2https://orcid.org/0000-0002-7555-2884Nataliia Shcheglova3Olena Karpenko4https://orcid.org/0000-0002-1943-8673Andriy Kytsya5https://orcid.org/0000-0002-7846-7183Vira Lubenets6https://orcid.org/0000-0001-6189-0084Nataliia Polish7https://orcid.org/0000-0002-1676-1053Department of Physical Chemistry of Fossil Fuels of the Institute of Physical-Organic Chemistry and Coal Chemistry named after L.M. Lytvynenko, NAS of UkraineDepartment of Physical Chemistry of Fossil Fuels of the Institute of Physical-Organic Chemistry and Coal Chemistry named after L.M. Lytvynenko, NAS of Ukraine; Foshan Tianheng New Material Technology Co.Department of Physical Chemistry of Fossil Fuels of the Institute of Physical-Organic Chemistry and Coal Chemistry named after L.M. Lytvynenko, NAS of UkraineDepartment of Physical Chemistry of Fossil Fuels of the Institute of Physical-Organic Chemistry and Coal Chemistry named after L.M. Lytvynenko, NAS of UkraineDepartment of Physical Chemistry of Fossil Fuels of the Institute of Physical-Organic Chemistry and Coal Chemistry named after L.M. Lytvynenko, NAS of UkraineDepartment of Physical Chemistry of Fossil Fuels of the Institute of Physical-Organic Chemistry and Coal Chemistry named after L.M. Lytvynenko, NAS of UkraineLviv Polytechnic National UniversityLviv Polytechnic National UniversityBackground. The accumulation of plastic waste negatively affects the environment and human health. Currently, one of the strategies to address this global ecological problem involves the utilization of biodegradable plastics instead in place of synthetic ones. Among them, polyhydroxyalkanoates (PHA) – microbial intracellular polymers – hold a significant position. Their advantages are biodegradability, biocompatibility, and favorable thermomechanical properties. Given these attributes, PHA has significant prospects for use in medicine, agriculture, and the food industry, in particular for packaging food products. Objective. Enchance the efficiency of bacterial synthesis of polyhydroxyalkanoates through nutrient media modification, obtain antimicrobial composites based on PHA, and determine their antimicrobial properties. Methods. The optimization of PHA biosynthesis involved selecting appropriate cultivation conditions, including carbon and nitrogen sources, cultivation time, and working volume. The isolation of PHA from bacterial biomass was achieved through chloroform extraction (mixing for 10 h at 35 °C, with a biomass-to-chloroform ratio of 1:50); followed by precipitation with double the volume of isopropanol. The resulting polymer was then dried to a constant weight at 60 °C. The hydrophobicity of the biopolymer was assessed using the water contact angle measurement. Composites of biopolymers with antimicrobials in the form of films were obtained using two methods: 1) solution casting method; and 2) layering the biocides onto the polymer film. The antimicrobial activity of the resulting composites was determined using the agar diffusion method. Results. Through the optimization of the mineral media and the change of cultivation conditions, it was possible to obtain 0.26–1.45 g/l of polyhydroxyalkanoates (5.1–34.0% PHA from biomass). The R. ruber UCM Ac-288 strain synthesized the maximum amount of biopolymer (34.0% PHA). This study established the ability of Gordonia bacteria to synthesize PHA for the first time. PHA compositions of optimal content were obtained, with hydrophobicity comparable to that of polyethylene packaging films. Antimicrobial properties of biopolymers composites with biocides have been substantiated. Conclusions. The bacterial synthesis of PHA was increased by modifying nutrient media. Composites based on PHA with biocides were developed. It was determined that these composites exhibit antimicrobial properties and high hydrophobicity. Consequently, they hold promise for use as biofilms for packaging and preserving food products.http://ibb.kpi.ua/article/view/280017polyhydroxyalkanoatesrhodococcus azotobactergordonia antimicrobial compositespackaging biofilms |
| spellingShingle | Nataliia Koretska Ihor Semeniuk Tetyana Pokynbroda Nataliia Shcheglova Olena Karpenko Andriy Kytsya Vira Lubenets Nataliia Polish Polyhydroxyalkanoates: Biosynthesis Optimization and Design of Antimicrobial Composites Innovative Biosystems and Bioengineering polyhydroxyalkanoates rhodococcus azotobacter gordonia antimicrobial composites packaging biofilms |
| title | Polyhydroxyalkanoates: Biosynthesis Optimization and Design of Antimicrobial Composites |
| title_full | Polyhydroxyalkanoates: Biosynthesis Optimization and Design of Antimicrobial Composites |
| title_fullStr | Polyhydroxyalkanoates: Biosynthesis Optimization and Design of Antimicrobial Composites |
| title_full_unstemmed | Polyhydroxyalkanoates: Biosynthesis Optimization and Design of Antimicrobial Composites |
| title_short | Polyhydroxyalkanoates: Biosynthesis Optimization and Design of Antimicrobial Composites |
| title_sort | polyhydroxyalkanoates biosynthesis optimization and design of antimicrobial composites |
| topic | polyhydroxyalkanoates rhodococcus azotobacter gordonia antimicrobial composites packaging biofilms |
| url | http://ibb.kpi.ua/article/view/280017 |
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