Community Structure and Diversity of Endophytic Bacteria in Melon (<i>Cucumis melo</i> L.) Seeds
Endophytic bacteria play a crucial role in the life cycle of Spermatophyta, and exploring the beneficial interactions between seeds and endophytic bacteria is an effective strategy for sustainable agricultural development and improved crop productivity. In this study, we employed Illumina Hiseq high...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/11/1195 |
| _version_ | 1797459133504421888 |
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| author | Kai Zeng Yuandong Li Zhou Wang Yongkang Du Mingqiang Fan Liqiong Xie |
| author_facet | Kai Zeng Yuandong Li Zhou Wang Yongkang Du Mingqiang Fan Liqiong Xie |
| author_sort | Kai Zeng |
| collection | DOAJ |
| description | Endophytic bacteria play a crucial role in the life cycle of Spermatophyta, and exploring the beneficial interactions between seeds and endophytic bacteria is an effective strategy for sustainable agricultural development and improved crop productivity. In this study, we employed Illumina Hiseq high-throughput sequencing technology to analyze the amplified fragments of microbial 16S rRNA sequences from melon seeds of MR-1, HH and their F<sub>1</sub> generation, which displayed varying levels of resistance to bacterial fruit blotch disease. Initially, we extracted endophytes from eight melon samples and performed sequencing, obtaining an average of 84,758 effective tags per sample. At the 97% similarity level, the number of operational taxonomic units (OTUs) derived from melon seed endophytes ranged from 447 to 492. After filtering out low-level OTUs, we obtained an average of 65,866 valid tags from the eight samples, representing approximately 160 genera and 167 species across eight phyla. It was found that the composition of the endophyte communities in the eight samples was basically similar, and their abundance showed some variability. Furthermore, we utilized the KO and COG databases to functionally predict the genes of endophytes in melon seeds. The analysis revealed a significant enrichment of genes associated with pathways related to protein synthesis, carbohydrate metabolism, substance transport, and metabolite synthesis. These findings greatly contribute to our understanding of endophytic bacteria in melon seeds and serve as a vital reference for the selection and breeding of high-quality melon seeds, as well as the prevention of seed diseases. |
| first_indexed | 2024-03-09T16:47:04Z |
| format | Article |
| id | doaj.art-8aba915376bd4083b4818bdc089836ff |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T16:47:04Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-8aba915376bd4083b4818bdc089836ff2023-11-24T14:45:12ZengMDPI AGHorticulturae2311-75242023-10-01911119510.3390/horticulturae9111195Community Structure and Diversity of Endophytic Bacteria in Melon (<i>Cucumis melo</i> L.) SeedsKai Zeng0Yuandong Li1Zhou Wang2Yongkang Du3Mingqiang Fan4Liqiong Xie5Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, ChinaEndophytic bacteria play a crucial role in the life cycle of Spermatophyta, and exploring the beneficial interactions between seeds and endophytic bacteria is an effective strategy for sustainable agricultural development and improved crop productivity. In this study, we employed Illumina Hiseq high-throughput sequencing technology to analyze the amplified fragments of microbial 16S rRNA sequences from melon seeds of MR-1, HH and their F<sub>1</sub> generation, which displayed varying levels of resistance to bacterial fruit blotch disease. Initially, we extracted endophytes from eight melon samples and performed sequencing, obtaining an average of 84,758 effective tags per sample. At the 97% similarity level, the number of operational taxonomic units (OTUs) derived from melon seed endophytes ranged from 447 to 492. After filtering out low-level OTUs, we obtained an average of 65,866 valid tags from the eight samples, representing approximately 160 genera and 167 species across eight phyla. It was found that the composition of the endophyte communities in the eight samples was basically similar, and their abundance showed some variability. Furthermore, we utilized the KO and COG databases to functionally predict the genes of endophytes in melon seeds. The analysis revealed a significant enrichment of genes associated with pathways related to protein synthesis, carbohydrate metabolism, substance transport, and metabolite synthesis. These findings greatly contribute to our understanding of endophytic bacteria in melon seeds and serve as a vital reference for the selection and breeding of high-quality melon seeds, as well as the prevention of seed diseases.https://www.mdpi.com/2311-7524/9/11/1195melon (<i>Cucumis melo</i> L.)seed endophyteshigh-throughput sequencinggene function prediction |
| spellingShingle | Kai Zeng Yuandong Li Zhou Wang Yongkang Du Mingqiang Fan Liqiong Xie Community Structure and Diversity of Endophytic Bacteria in Melon (<i>Cucumis melo</i> L.) Seeds Horticulturae melon (<i>Cucumis melo</i> L.) seed endophytes high-throughput sequencing gene function prediction |
| title | Community Structure and Diversity of Endophytic Bacteria in Melon (<i>Cucumis melo</i> L.) Seeds |
| title_full | Community Structure and Diversity of Endophytic Bacteria in Melon (<i>Cucumis melo</i> L.) Seeds |
| title_fullStr | Community Structure and Diversity of Endophytic Bacteria in Melon (<i>Cucumis melo</i> L.) Seeds |
| title_full_unstemmed | Community Structure and Diversity of Endophytic Bacteria in Melon (<i>Cucumis melo</i> L.) Seeds |
| title_short | Community Structure and Diversity of Endophytic Bacteria in Melon (<i>Cucumis melo</i> L.) Seeds |
| title_sort | community structure and diversity of endophytic bacteria in melon i cucumis melo i l seeds |
| topic | melon (<i>Cucumis melo</i> L.) seed endophytes high-throughput sequencing gene function prediction |
| url | https://www.mdpi.com/2311-7524/9/11/1195 |
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