Molecular cloning, characterization and promoter analysis of a novel MDR-type ABC transporter gene (GbMDR1) from Ginkgo biloba
A novel MDR-type ABC transporter cDNA (designated as Gbmdr1) was cloned and characterized for the first time from the gymnosperm plant species, Ginkgo biloba, using RACE technique. The full-length cDNA of Gbmdr1 was 4275 bp, and it contained a 3840 bp open reading frame (ORF) encoding a pol...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2013-01-01
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| Series: | Archives of Biological Sciences |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2013/0354-46641304339L.pdf |
| _version_ | 1818475105822441472 |
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| author | Liu Dong-Hui Zeng Yan Wei Xing-Cheng Fan Ye-Ming Shi Xiu-Yan |
| author_facet | Liu Dong-Hui Zeng Yan Wei Xing-Cheng Fan Ye-Ming Shi Xiu-Yan |
| author_sort | Liu Dong-Hui |
| collection | DOAJ |
| description | A novel MDR-type ABC transporter cDNA (designated as Gbmdr1) was cloned and
characterized for the first time from the gymnosperm plant species, Ginkgo
biloba, using RACE technique. The full-length cDNA of Gbmdr1 was 4275 bp, and
it contained a 3840 bp open reading frame (ORF) encoding a polypeptide of
1279 amino acid with a predicted isoelectric point of 8.22 and molecular mass
of 139.6 kDa. The deduced GbMDR1 protein consisted of four domains including
two TMDs (74-351, 716-993) and two NBDs (428-607, 1067-1251). The promoter of
the Gbmdr1 was also cloned by the Genome Walking method. Sequence analysis
demonstrated that there were many regulatory elements in the Gbmdr1 promoter
and the TATA box was located at -52~-56 bp upstream of the transcriptional
start site. Sequence alignment and molecular evolution analysis revealed that
GbMDR1 was a plant MDR-like ABC transporter protein, and that it has a
further relationship with most other MDRs of plant species. Southern blot
analysis indicated that Gbmdr1 belonged to a small multi-gene family. Tissue
expression analysis indicated that Gbmdr1 expression was high in stems and
leaves but low in roots. These results show that GbMDR1 is a MDR-like ABC
transporter protein that may be involved in the transport and accumulation of
secondary metabolites. |
| first_indexed | 2024-12-10T03:59:04Z |
| format | Article |
| id | doaj.art-8abac6069e3d42b6bb1eff1daee20198 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-10T03:59:04Z |
| publishDate | 2013-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-8abac6069e3d42b6bb1eff1daee201982022-12-22T02:03:02ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392013-01-016541339135210.2298/ABS1304339L0354-46641304339LMolecular cloning, characterization and promoter analysis of a novel MDR-type ABC transporter gene (GbMDR1) from Ginkgo bilobaLiu Dong-Hui0Zeng Yan1Wei Xing-Cheng2Fan Ye-Ming3Shi Xiu-Yan4Department of Biochemistry and Molecular Biology, Shenyang Medical College, Shenyang, ChinaDepartment of Immunology, Shenyang Medical College, Shenyang, ChinaDepartment of Immunology, Shenyang Medical College, Shenyang, ChinaDepartment of Biochemistry and Molecular Biology, Shenyang Medical College, Shenyang, ChinaDepartment of Biochemistry and Molecular Biology, Shenyang Medical College, Shenyang, ChinaA novel MDR-type ABC transporter cDNA (designated as Gbmdr1) was cloned and characterized for the first time from the gymnosperm plant species, Ginkgo biloba, using RACE technique. The full-length cDNA of Gbmdr1 was 4275 bp, and it contained a 3840 bp open reading frame (ORF) encoding a polypeptide of 1279 amino acid with a predicted isoelectric point of 8.22 and molecular mass of 139.6 kDa. The deduced GbMDR1 protein consisted of four domains including two TMDs (74-351, 716-993) and two NBDs (428-607, 1067-1251). The promoter of the Gbmdr1 was also cloned by the Genome Walking method. Sequence analysis demonstrated that there were many regulatory elements in the Gbmdr1 promoter and the TATA box was located at -52~-56 bp upstream of the transcriptional start site. Sequence alignment and molecular evolution analysis revealed that GbMDR1 was a plant MDR-like ABC transporter protein, and that it has a further relationship with most other MDRs of plant species. Southern blot analysis indicated that Gbmdr1 belonged to a small multi-gene family. Tissue expression analysis indicated that Gbmdr1 expression was high in stems and leaves but low in roots. These results show that GbMDR1 is a MDR-like ABC transporter protein that may be involved in the transport and accumulation of secondary metabolites.http://www.doiserbia.nb.rs/img/doi/0354-4664/2013/0354-46641304339L.pdfABC transporter-Ginkgo biloba-MDR-Gbmdr1-molecular cloning |
| spellingShingle | Liu Dong-Hui Zeng Yan Wei Xing-Cheng Fan Ye-Ming Shi Xiu-Yan Molecular cloning, characterization and promoter analysis of a novel MDR-type ABC transporter gene (GbMDR1) from Ginkgo biloba Archives of Biological Sciences ABC transporter-Ginkgo biloba-MDR-Gbmdr1-molecular cloning |
| title | Molecular cloning, characterization and promoter analysis of a novel MDR-type ABC transporter gene (GbMDR1) from Ginkgo biloba |
| title_full | Molecular cloning, characterization and promoter analysis of a novel MDR-type ABC transporter gene (GbMDR1) from Ginkgo biloba |
| title_fullStr | Molecular cloning, characterization and promoter analysis of a novel MDR-type ABC transporter gene (GbMDR1) from Ginkgo biloba |
| title_full_unstemmed | Molecular cloning, characterization and promoter analysis of a novel MDR-type ABC transporter gene (GbMDR1) from Ginkgo biloba |
| title_short | Molecular cloning, characterization and promoter analysis of a novel MDR-type ABC transporter gene (GbMDR1) from Ginkgo biloba |
| title_sort | molecular cloning characterization and promoter analysis of a novel mdr type abc transporter gene gbmdr1 from ginkgo biloba |
| topic | ABC transporter-Ginkgo biloba-MDR-Gbmdr1-molecular cloning |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2013/0354-46641304339L.pdf |
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